Belonging to the tumor therapy protein related technical field, the invention specifically relates to a dendritic cell (DC) targeted affinity peptide TY peptide and application thereof. The TY peptideis 12 peptide, and the specific amino acid sequence is TITHFQFGPTVY. Based on the TY peptide, the invention further designs a novel nano-transportation system capable of targeting DC on the basis ofMSN, and loads the model antigen OVA and immunoadjuvant CpG to prepare the nano-carrier MSN(mesoporous silica nanoparticles)-TY/OVA/CpG successfully. Experiments indicate that: the MSN-TY/OVA/CpG canbe effectively ingested by immature DC, thus improving the efficiency of antigen ingestion by DC, promoting the maturation of DC and effectively activating DC, also effectively promoting antigen processing and presentation, stimulating secretion of cytokines, and further triggering antigen-specific CTL immunoreaction and anti-tumor immunoreaction.