Peripheral blood exosome-sourced liver cancer diagnosis and prognosis marker and applications thereof

A technology of markers and peripheral blood, applied in the field of biomedicine, can solve the problems of unsatisfactory sensitivity and specificity, and achieve the effects of improving sensitivity and specificity, accurate quantification, and easy detection

Inactive Publication Date: 2015-04-22
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sensitivity and specificity of these two methods are not very satisfactory

Method used

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  • Peripheral blood exosome-sourced liver cancer diagnosis and prognosis marker and applications thereof
  • Peripheral blood exosome-sourced liver cancer diagnosis and prognosis marker and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Differential expression of Rab27A protein and Rab27B protein derived from peripheral blood exosome

[0060] 1.1 Collection of peripheral blood samples

[0061] The inventor collected a large number of peripheral blood samples of liver cancer patients and liver cirrhosis patients in the First Affiliated Hospital of the Chinese People's Liberation Army in January 2013 (the samples used for research were collected, sampled, sub-packaged, and preserved in the same period). For the sorting of sample data, the inventor selected 50 cases of liver cancer patient plasma collected randomly in the same hospital and as far as possible in the same department, and 50 cases of normal person plasma randomly collected in the same hospital and experimental group at the same period. Patients who have not received special treatment, such as antiviral and antitumor treatment, or who have stopped the above treatment for more than 9 weeks, take fasting EDTA anticoagulated peripheral...

Embodiment 2

[0079] Example 2 Correlation analysis between the expression of Rab27A protein and Rab27B protein derived from peripheral blood exosome and liver cancer

[0080] According to the method of Example 1, peripheral blood samples of 200 liver cancer patients were collected, and peripheral blood samples of 200 normal people were collected at the same time, and the contents of Rab27A protein and Rab27B protein in the peripheral blood samples were measured, and the Rab27A of liver cancer patients and normal people were counted. The positive rate of protein and the negative rate of Rab27B protein expression.

[0081] The results of multivariate Logistic regression analysis showed that abnormal levels of Rab27A protein and Rab27B protein in peripheral blood exosome had a clear correlation with liver cancer. The analysis results are shown in Table 1 and Table 2.

[0082] Table 1 Correlation analysis between abnormal Rab27A protein content and liver cancer

[0083] normal pe...

Embodiment 3

[0086] Example 3 The relationship between the expression of Rab27A protein and Rab27B protein derived from peripheral blood exosome in patients with liver cancer and prognosis

[0087] 200 cases of liver cancer patients in Example 2 were followed up, their survival period was observed, and statistics were carried out. The results showed that the one-year survival rate of patients with positive Rab27A expression was 38%, and the one-year survival rate of patients with negative Rab27A expression was 70%. , indicating that the overall survival time of patients with positive expression of Rab27A was significantly shorter than that of patients with negative expression of Rab27A (P<0.05); the one-year survival rate of patients with positive expression of Rab27B was 72%, and the one-year survival rate of patients with negative expression of Rab27B was 30%. %, indicating that the overall survival time of patients with negative Rab27B expression was significantly shorter than that of pa...

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Abstract

A peripheral blood exosome-sourced liver cancer diagnosis and prognosis marker is disclosed. The marker is exosome-sourced Rab27A protein and / or Rab27B protein. Through western blot detection, it is found that the positive expression rate of the Rab27A protein in a peripheral blood exosome of a patient with liver cancer is obviously higher than that of a common person, and the negative expression rate of the Rab27B protein in the peripheral blood exosome of the patient with liver cancer is obviously higher than that of a common person. Analysis about the abovementioned experiment results in combination with clinical data shows that content abnormity of the Rab27A protein and the Rab27B protein is significantly correlated to occurrence and development of liver cancer; and survival time of patients with positive expression of the Rab27A protein and negative expression of the Rab27B protein is shortened. The diagnosis and prognosis marker provides a method having higher sensitivity and higher specificity for liver cancer diagnosis and prognosis in clinic.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to liver cancer diagnostic and prognostic markers derived from human peripheral blood exosomes and applications thereof; in particular, to the Rab27A / B protein derived from human blood exosomes as liver cancer diagnostic and prognostic markers and its preparation for diagnosing liver cancer and judging Application of Liver Cancer Prognosis Kit. Background technique [0002] Primary liver cancer (HCC) is one of the top ten common tumors worldwide, with a very high mortality rate, and about one million people die from this disease every year. Finding tumor markers related to the progression of primary liver cancer and then guiding the clinical practice will be of great significance to patients with liver cancer. [0003] The exosome is a 30-100nm membranous sac-like structure secreted by a variety of cells. Tumor cells can secrete more exosomes than normal cells, and the exosomes secreted e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57438G01N33/57484G01N33/6803
Inventor 肖文华董伟伟
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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