Kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof

A technology for identification and detection of plasma cells, which is applied in measurement devices, particle and sedimentation analysis, instruments, etc. It can solve the problems of inability to achieve group gates and further analysis of clonality, inability to include antibodies at the same time, and increase in sample volume. Broad application prospects and clinical significance, ease of standardization and standardization, and the effect of reducing repeated use

Active Publication Date: 2016-01-06
PEOPLES HOSPITAL PEKING UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, most of the literature reports use a combination of 3-4 color antibodies. Except for the gating antibody, only 1-2 other antibodies can be detected in each tube. If you want to detect multiple antibodies to determine whether the plasma cells are abnormal, you need to use the gating antibody repeatedly. , about 3-5 sets of antibody combinations are required, and a tota

Method used

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  • Kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof
  • Kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof
  • Kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof

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[0019] A kit for identifying and detecting normal plasma cells and clonal plasma cells, used in conjunction with a flow cytometer, is characterized in that the kit is equipped with a combination of monoclonal antibodies against the following substances: Ig, κLightChain / Ig, λLightChain, CD19, CD117, CD138, CD56, CD38, and CD45 were labeled with fluorescein in the following order: FITC / PE, PE-Cy5, PE-Cy7, APC, APC-Cy7, V450, and V500. See Table 1 for information on the fluorescein labeling and composition of each monoclonal antibody.

[0020] Table 1 Flow monoclonal antibody information

[0021]

[0022] The kit also includes erythrocyte lysing solution (10×, product number: 555899, BD company), fixative / membrane breaking agent (product number: GAS006, Multisciences company, including 20ml A solution and 20ml B solution, A solution is a cell fixative solution , the role is to fix the cells; the B solution is the membrane-breaking solution, so that the antibody can enter th...

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Abstract

The invention discloses a kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof. The kit is matched with a flow cytometry for usage. The kit is equipped with monoclonal antibody combination according to the following substances: Ig, kappa Light Chain, Ig, lambda Light Chain, CD19, CD117, CD138, CD56, CD38 and CD45. CD38 and CD138 are first used for the identification of the plasma cells; CD45, CD56, CD19 and CD117 are employed for recognizing normal or abnormal state of the plasma cell surface; gating is conducted on normal and abnormal phenotypes (R4 and R5); and then the expression ratio of cKappa and cLambda in normal and abnormal phenotypes are respectively observed. The kit of the invention is used to identify all cPCs and has the advantages of high accuracy, fast analysis and simple method.

Description

[0001] The invention belongs to the structural design of a special kit in the process of cell phenotype confirmation, and in particular relates to a kit for identifying clonal plasma cell phenotypes using an eight-color antibody combination and its application. Background technique [0002] Plasma cell disease refers to a group of diseases in which clonal plasma cells (clonal plasma cells, cPCs) proliferate excessively and produce a large amount of monoclonal immunoglobulin, and cPCs are the root cause of plasma cell disease. Immobilized immunoglobulin electrophoresis or detection of free light chains can help determine the presence, but not the proportion, of clonal plasma cells; cPCs are not diagnostic if they are non-secreting, ie, do not synthesize immunoglobulin. Traditional bone marrow morphology methods can identify the proportion of abnormally shaped or naive plasma cells, but cannot determine their clonality. In certain diseases, such as monoclonal gammopathy of undet...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/533G01N15/14
CPCG01N15/14G01N33/533G01N33/577
Inventor 刘艳荣王亚哲常艳郝乐袁晓英黄晓军
Owner PEOPLES HOSPITAL PEKING UNIV
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