Flavivirus-detecting gene chip probe and gene chip detection method

Abstract

The invention provides a group of flavivirus-detecting gene chip probes and a general detection method. The probes include a Tick borne encephalitis probe, a Denguo virus probe and a Japanese encephalitis probe. A general primer sequence of flaviviruses capable of specific reverse transcription is designed, and flavivirus specific primers are utilized to perform reverse transcription for guiding the synthesis of viral genome so as to reduce interference in host cell gene components to the fullest extent and improve the specificity and accuracy of detection results. The invention can be used for the gene amplification of all flaviviruses and the gene chip detection of Tick borne encephalitis, Denguo virus and Japanese encephalitis.

Description

technical field [0001] The invention relates to a group of gene chip probes capable of detecting flaviviruses and a general detection method for flaviviruses gene chips. Background technique [0002] At present, detection methods for viral pathogens are still limited to traditional immunofluorescence detection, ELISA and PCR detection. Although they have good specificity, they can only detect one or several pathogens at the same time, which is time-consuming and laborious. Gene chip technology has the characteristics of fast and high-throughput. It can design detection probes for multiple viruses and spot them on the same chip to detect multiple pathogens. Compared with traditional methods, the detection efficiency can be greatly improved. [0003] At present, there are direct immunofluorescence method, indirect immunofluorescence method, real-time quantitative PCR method, RT-PCR detection method, etc. for the detection of dengue virus, Japanese encephalitis virus, forest e...

AI Technical Summary

Problems solved by technology

[0004] The common problem at present is that the detection results are prone to false positive or false negative when using the gene chip method to detect pathogens

The reason lies in the amplification method of the virus gene. At present, the random PCR amplification method is commonly used. Because the virus has the characteristics of intracellular parasitic, the DNA and RNA of the host cell will inevitably be extracted during the RNA extraction process. The genome of eukaryotes is usually larger than The viral genome is dozens of times larger, and in the subsequent random amplification process, the genes with high content will be amplified preferentially, which interferes with the effective amplification of the viral genome, resulting in false positive or false negative test results

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