Method for cultivating and collecting Bursaphelenchus nematodes and device thereof

Abstract

The present invention relates to a bursaphelenchus nematode culture and collection method and a device thereof, pertaining to the technical field of nematode culture. The device comprises a tissue culture bottle (1), a tissue culture bottle cover (2) and an eccentric pipe body which is embedded on the tissue culture bottle cover (2) and consists of an eccentric pipe cover (3) and an eccentric pipe (4). The method is improved on the basis of the conventional culture and collection method. Compared with the prior art, the present invention has the advantages of simple structure, simplifying the complex operating procedures of the conventional nematode culture and experimental nematode selection, lowering the experimental difficulty, saving the experiment time, improving the purity of experimental nematode and the accuracy of experimental results, etc. The method and the device of the present invention can be widely applied to the culture of bursaphelenchus nematode and the collection of high-purity nematode group.

Description

Technical field: [0001] The invention relates to a method and device for cultivating and collecting nematodes of the genus Cyclops, belonging to the technical field of nematode cultivation. Background technique: [0002] The cultivation of plant pathogenic nematodes is an important experimental link in the teaching and scientific research of plant pathology. For B. xylophilus and B. xylophilus in the genus Umberella, the most commonly used culture method is the culture method of Botrytis cinerea on PDA medium, which is usually carried out in glass petri dishes. The specific method is to first inoculate Botrytis cinerea on a sterile solid PDA medium, and when the culture dish is covered with Botrytis cinerea, inoculate the required nematodes. After about two weeks of cultivation, a large number of Botrytis cinerea mycelium can be seen. When the nematode feeds, the surface of the culture medium can be washed with sterile water to recover the nematode. The main disadvantages o...

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Problems solved by technology

The specific method is to first inoculate Botrytis cinerea on a sterile solid PDA medium, and when the culture dish is covered with Botrytis cinerea, inoculate the required nematodes. After about two weeks of cultivation, a large number of Botrytis cinerea mycelium can be seen. When the nematode feeds, the surface of the culture medium can be washed with sterile water to recover the nematode. The main disadvantages of this method are: 1. A large amount of Botrytis cinerea mycelia and spores are mixed in the recovered nematode suspension; 2. The obtained The dead worms in the nematode suspension account for a considerable proportion; 3. The amount of worms obtained is relatively small

[0003] Through literature search, there is no public report identical with the present invention

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