Modeling method of helicobacter pylori infection animal model

A technology of Helicobacter pylori infecting animals, which is applied in the field of microbiology and experimental zoology, can solve the problems of long modeling time, high cost, and difficulty in establishing a laboratory, and achieve long infection time, less operation times, and short experiment time Effect

Active Publication Date: 2013-12-18
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is that, in order to overcome the poor controllability, instability, low success rate, long modeling time, complicated operation and high cost of establishing an animal model of Helicobacter pylori infection in the prior art, general experiments It is difficult to establish and other defects in the chamber, and provides a method for establishing an animal model of Helicobacter pylori infection

Method used

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  • Modeling method of helicobacter pylori infection animal model
  • Modeling method of helicobacter pylori infection animal model
  • Modeling method of helicobacter pylori infection animal model

Examples

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Effect test

Embodiment 1

[0039] 4-week-old female C57BL / 6 mice, 48 mice, weighing 11g±1-2g, were fed with mixed antibiotics for 7 days (protocol: gentamicin 0.062 million units / mouse / day, vancomycin 1.7mg / mouse / day , nystatin 07,700 units / mouse / day) → normal feeding for one week → inoculation of Helicobacter pylori: after the mice were fasted for 24 hours, 0.18mL / mouse / time of Helicobacter pylori bacterial solution was administered orally, and the bacterial density was 1×10 9 cfu / mL, eat after 8 hours. Once every other day, 3 times in total→feeding for two weeks→sampling to confirm. The inoculation success rate of the modeling random inspection is 100%, and the modeling survival rate is 96%.

Embodiment 2

[0041] 4-week-old female C57BL / 6 mice, 48 mice, weighing 11g±1-2g, were fed with mixed antibiotics for 7 days (protocol: gentamicin 0.058 million units / mouse / day, vancomycin 1.2mg / mouse / day , nystatin 07,200 units / mouse / day) → normal feeding for 6 days → inoculation of Helicobacter pylori: after the mice were fasted for 24 hours, 0.2 mL / mouse / time of Helicobacter pylori bacterial solution was administered orally, and the bacterial density was 1×10 9 cfu / mL, eat after 8 hours. Once every other day, 3 times in total→feeding for two weeks→sampling to confirm. The inoculation success rate of modeling random inspection is 100%, and the modeling survival rate is 100%.

Embodiment 3

[0043] 4-week-old female C57BL / 6 mice, 48 mice, weighing 11g±1-2g, were fed with mixed antibiotics for 3 days (protocol: gentamicin 0.062 million units / mouse / day, vancomycin 1.5mg / mouse / day , nystatin 07,700 units / mouse / day) → normal feeding for 6 days → inoculation of Helicobacter pylori: after the mice were fasted for 24 hours, 0.22mL / mouse / time of Helicobacter pylori bacterial solution was administered orally, and the bacterial density was 1×10 9cfu / mL, eat after 12 hours. 1 time every other day, 2 times in total→feeding for two weeks→sampling to confirm. The inoculation success rate of modeling random inspection is 100%, and the modeling survival rate is 100%.

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Abstract

The invention discloses a modeling method of a helicobacter pylori infection animal model. The modeling method comprises the steps of: 1, by using a 4-weeks old female C57BL/6 mouse as the experimental animal, performing intra-gastric administration for 3 to 7 days by mixed antibiotic, wherein the mixed antibiotic is composed of gentamicin, vancomycin and nystatin; 2, raising the C57BL/6 mouse which has been subjected to the intra-gastric administration in the step 1, for 6 to 8 days; 3, inoculating helicobacter pylori: fasting the C57BL/6 mouse obtained in the step 2, carrying out intra-gastric administration by helicobacter pylori, and feeding after 8 to 12 hours, wherein the inoculating in the step 3 is performed one time every other day, and performed 2 to 4 times in total; and 4, raising the mouse which has been inoculated for 2 to 4 times in the step 3. In the modeling method provided by the invention, the experimental time is short, the operation time is less, the order of magnitude of the helicobacter pylori infection is high, and the infection time is endurable; the model can be widely used for the method of helicobacter pylori infection animal in related researches.

Description

technical field [0001] The invention relates to the fields of microbiology and experimental zoology, in particular to a method for establishing an animal model infected by Helicobacter pylori. Background technique [0002] Helicobacter pylori (Hp) is recognized as the main pathogenic factor of gastritis, peptic ulcer and gastric cancer, and human is the only susceptible host for Hp infection. In developed countries, the infection rate of Helicobacter pylori is about 50%, while in developing countries it reaches more than 60%. To this end, domestic and foreign researchers are establishing animal models of Helicobacter pylori infection to study the pathogenic mechanism, specific prevention and treatment of Helicobacter pylori, and the efficacy of some drugs or microecological preparations. [0003] In domestic and foreign reports on animal models of Helicobacter pylori infection, guinea pigs and nude mice are used as experimental animals. Due to problems such as easy death an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/14A61K35/74A61K31/7036A61K31/7048
Inventor 苗君莅郭本恒刘振民吴正钧于鹏
Owner BRIGHT DAIRY & FOOD
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