Method of whole manual culture for chinese caterpillar fungus

A technology of artificial cultivation of Cordyceps sinensis, which is applied in the biological field, can solve the problems such as the inability to obtain Cordyceps sinensis, and achieve the effect of overcoming long growth time, large individuals, and high success rate of infection

Active Publication Date: 2007-04-25
张雪峰
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Some biological experts use liquid submerged fermentation to solve the market demand for Cordyceps sinensis. Because the application of liquid submerged fermentation can only produce the mycelium of Cordyceps sinensis, it can only be processed into a preparation of Cordyceps sinensis mycelium, and it is impossible to obtain the same shape as the natural one. Cordyceps sinensis, and many people recognize that it is like the natural shape of Cordyceps sinensis
However, there is no relevant report on the full artificial culture of Cordyceps sinensis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of whole manual culture for chinese caterpillar fungus
  • Method of whole manual culture for chinese caterpillar fungus
  • Method of whole manual culture for chinese caterpillar fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The full artificial culture method of embodiment 1 Cordyceps sinensis of the present invention

[0043] 1.1 Collection of samples

[0044] Cordyceps samples were collected from alpine shrub meadows at an altitude of 3,500 to 5,000 meters in Ganzi Prefecture, Sichuan.

[0045] 1.2 Separation of strains

[0046]1.2.1 Basic medium for separation: potato, glucose, agar, water, plus peptone (referred to as PDA + peptone) and potato, glucose, agar, water, plus silkworm chrysalis juice.

[0047] Its weight proportion: potato 200g, glucose 20g, agar 20g, peptone 10g, water 1000ml.

[0048] 1.2.2 Separation method

[0049] Sclerotia (worm body) and sub-seat separation: Sclerotia and sub-seats are surface-sterilized with 0.1% mercury chloride solution, washed with sterile water, cut into small sections or thin slices, placed on a culture dish with medium, and kept at 8- Incubate at 18°C.

[0050] Separation of ascospores: take the head of the ascospore, disinfect the surface...

Embodiment 2

[0092] The quality control of the Cordyceps sinensis that embodiment 2 inventive method cultivates

[0093] Select natural Cordyceps sinensis for comparison with the full artificial cultivation of Cordyceps sinensis of the present invention, take amino acid as example: sample is hydrolyzed through 6NHCl, temperature 110 ℃, time 24hr, analyze with Hitachi 835-50 amino acid analyzer, the result is as follows:

[0094] Natural Cordyceps %

[0095] The amino acid composition of the Cordyceps sinensis cultivated by the method of the invention is slightly higher than that of natural Cordyceps sinensis.

[0096] Adenosine (C 10 h 13 N 5 o 4 ) active ingredients as an example to the determination of the whole artificial culture of Cordyceps sinensis ("Chinese Pharmacopoeia" stipulates the determination of adenosine (C 10 h 13 N 5 o 4 ) content shall not be less than 0.010%):

[0097]

[0098] Through the measurement, it shows that the adenosine active ingre...

Embodiment 3

[0099] Cordyceps sinensis pharmacodynamics test cultivated by the method of the present invention in embodiment 3

[0100] Get 300g of Cordyceps sinensis cultivated by the method of the present invention, directly powder it, pack into capsules, and make capsules, and each capsule contains 0.3g of Cordyceps sinensis medicinal material.

[0101] The beneficial effect of the present invention is proved by regulating immunity, improving sleep, improving the pharmacodynamics test example of sleep quality now.

[0102] According to the test: the fully artificially cultivated Cordyceps sinensis has a sedative effect on the central nervous system; it has a significant effect on the respiratory system to expand the bronchus, and can significantly enhance the effect of adrenaline, and it also has the effect of eliminating phlegm, relieving asthma and preventing emphysema.

[0103] For the cardiovascular system, it can reduce myocardial oxygen consumption, improve myocardial ischemia, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
elevationaaaaaaaaaa
depthaaaaaaaaaa
Login to view more

Abstract

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

Description

technical field [0001] The invention provides a fully artificial culture method for Cordyceps sinensis, which belongs to the field of biology. Background technique [0002] Cordyceps sinensis is a kind of precious fungus Chinese medicinal material, which is produced by the larvae of Hepialus Spp. which only grow in alpine shrub meadows at 3500-5000 meters infested by Hirsutella Sinensis Liu, Guo, Yu et Zeng. Sexual type. Dig out when the spores are unearthed and the spores are not dispersed, remove the fibrous mycelium wrapped around the insect body, and dry in the sun or at low temperature. [0003] The larvae of the host of Cordyceps sinensis live in the soil. After the Mortierella sinensis invades the larvae, it will continue to grow and multiply in the larvae with the circulation of body fluids, filling the entire larvae to form a sclerotia, and finally the larvae will produce a sub-seat from the head of the larvae. . [0004] Cordyceps sinensis is mainly distributed ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645A61K36/068A61P25/20A61P37/02
Inventor 俞永信
Owner 张雪峰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap