Wheat genetic gene transformation method

Abstract

The invention provides a wheat genetic gene transformation method. After agrobacterium attaches to the wound tissue of plants, through the interaction between the agrobacterium and the wound tissue of the plants, agrobacterium can form small cellulose fibrils which are fixed to cell walls of the plants, meanwhile, the injured plant tissue is induced to release some phenolic compounds, such as acetosyringone (AS) and hydroxy acetosyringone (AS-OH), and the phenolic compounds are taken as signal molecules to activate the transcription of other Vir genes and activate T-DNA with target gene segments of meloidogynosis to be inserted into chromosomes of the callus. The result shows that the genotype is the key factor influencing the formation and differentiation and regeneration of the wheat explant callus; the factors including the concentration of inoculated bacterium fluid, the infection time, the co-culture conditions and the like have great influences on the genetic transformation efficiency of the wheat explant callus; the obtained transgenic wheat regenerated plant lays a solid foundation for the genetic transformation research of wheat and the genetic analysis for the transgenic progenies.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a method for genetic transformation of wheat. Background technique [0002] Wheat is an annual or perennial herbaceous plant of the genus Triticum (Triticum) of the grass family (Gramineae), and is one of the important food crops in the world. It is a new direction of wheat genetic breeding to use plant genetic engineering technology to improve wheat genetics to increase wheat yield, improve quality, and enhance resistance to diseases and insect pests and stress. [0003] Since the first transgenic crop was obtained in 1983, crop transgenic technology has developed rapidly, and genetically modified crop breeding has become an effective supplement to conventional breeding. At present, almost all crops have carried out transgenic research. The breeding goals involve many aspects such as high yield, high quality, high efficiency, combined resistance and multi-purp...

AI Technical Summary

Problems solved by technology

This is mainly caused by the following reasons: first, the lack of effective in vitro regeneration system

The problems of low plant regeneration rate and strong genotype dependence in wheat tissue culture have always been the main factors that limit the success of transgenic and greatly increase the number of transgenic wheat; second, the explant material used for gene transformation is relatively small

Immature embryos are the main explants for genetic transformation of wheat, but they are often limited by the season of harvesting; third, relying too much on the gene gun method

At present, more than 90% of transgenic wheat is obtained by biolistic method, but there are many shortcomings in the biolistic method, which limits the development of wheat transgenic; fourth, there is a lack of systematic and in-depth research on the mechanism of Agrobacterium transformation of monocotyledonous plants, especially transformed wheat ; Fifth, wheat itself is an allohexaploid, its genome is large and complex, and the phenomenon of silencing and modification of imported foreign genes is very serious

[0005] Although many of the above factors have delayed the development of wheat transgenic technology, with the establishment of multiple transgenic systems, the development speed of wheat transgenic research will be accelerated