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Method for rapidly detecting pathogenic anthracnose of dracaena sanderiana and application thereof

A technology of anthrax and pathogenic bacteria, applied in the field of genetic engineering, can solve problems such as time-consuming, difficult to achieve pathogenic bacteria, strong experience, etc., and achieve the effect of early and effective control measures

Inactive Publication Date: 2017-07-04
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional pathogen identification methods are time-consuming, low-sensitivity, and highly empirical. It takes several days to isolate and identify pathogens from diseased plants, making it difficult to monitor the occurrence of diseases in a timely manner and effectively control the spread of pathogens and epidemics

Method used

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  • Method for rapidly detecting pathogenic anthracnose of dracaena sanderiana and application thereof
  • Method for rapidly detecting pathogenic anthracnose of dracaena sanderiana and application thereof
  • Method for rapidly detecting pathogenic anthracnose of dracaena sanderiana and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0020] 1. Collection of fungal hyphae

[0021] Take the preserved identified strains (C.dracaehophilum, C.cliviae, C.petchii, C.fructicola, C.karstii, C.boninense, C.asianum, C.acutatum, C.truncatum, C.brevisporum, C.aenigma , C. liriopes, C. scovillei, A.alternata, Fusarium sp., Curvularia sp., Bipolaris sp., Exserohilum sp., Drechslera sp.) were inoculated on PDA plates, grown at 25°C for 5 days, picked fresh mycelia, Inoculate in a 250mL Erlenmeyer flask filled with 1 / 3 volume of PDB. 25°C, 120 r / min, culture for 3-4 days, until a large number of mycelium clusters are formed. Filter with double gauze, rinse continuously with distilled water, blot dry with filter paper, and store in a 1.5mL centrifuge tube at -20°C for later use.

[0022] 2. Extraction of Fungal DNA

[0023] Using the improved CTAB method to extract fungal hyphae DNA, the specific steps are as follows:

[0024] (1) Take an appropriate amount of mycelium (about 0.5g) in a mortar, add liquid nitrogen and q...

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PUM

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Abstract

The invention belongs to the field of gene engineering technology and specifically relates to a method for rapidly detecting pathogenic anthracnose of dracaena sanderiana and application thereof. The method comprises the following steps: (1) extracting total DNA of a sample to be detected; (2) carrying out PCR amplification on a target fragment by using the total DNA as a template and using a specific primer ZM3-7F and a specific primer ZM3-7R as primers; and (3) carrying out electrophoresis detection on the amplification product obtained in the step (2) in 1.2% agarose gel, and analyzing results, wherein the specific primer ZM3-7F sequence is as shown in the SEQ ID NO:1 and the specific primer ZM3-7R sequence is as shown in the SEQ ID NO:2. The method of the invention lays the foundation for rapidly identifying anthracnose of dracaena sanderiana and accurately monitoring latent infection of the pathogen, is beneficial to timely and effectively take control measures.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to a method for rapidly detecting pathogens using specific primers, in particular to a method for rapidly detecting the pathogenic bacteria of lucky bamboo anthracnose and its application. Background technique [0002] The pathogenic bacteria of anthracnose of rich bamboo (Dracaena anthracnose) are half-known fungi, Anthracnose genus, Dracaenhophilum Colletotrichum dracaenhophilum D.F. Farr & M.E. Palm. On the host, conidia discs are buried under the epidermis, round or oval, and after breaking through the epidermis, yellow conidia piles are formed, with bristles, 2-4 cells, brown to light brown, tapering from base to apex , 118-165µm×3.5-7.3µm, conidiophores light brown to colorless, cylindrical, 1-3 cells, smooth surface, 43-55×4.5-7.3µm, average: 47×5.5µm. Conidia monospore, smooth, colorless, blunt at both ends, cylindrical or club-shaped, occasionally curved, 19.8-30....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6895
Inventor 武海燕张猛张亚龙耿月华那日松施艳马乐乐汪敏
Owner HENAN AGRICULTURAL UNIVERSITY
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