Corn southern leaf blight field natural inducement technology and application of technology

Abstract

The invention relates to the technical field of crop disease research, in particular to a corn southern leaf blight field natural inducement technology and application of the technology. The technology basically includes the steps that plastic film mulching is conducted; individual core seedlings which all have 3-4 leaves and 1 core are planted in two rows, wherein the line spacing is 0.4-0.5 m; in the stage when each corn plant in the field has 7-8 leaves, fresh corn southern leaf blight diseased plants artificially inoculated in a greenhouse serve as a field inducement bacterium source and horizontally placed on moist surfaces between the corn plants, and field normal irrigation is used for keeping soil moist. The infected corn southern leaf blight diseased plants which are artificially inoculated serve as the inducement bacterium source, operation is easy, and the inducement bacterium source is easy to obtain. The structure of the inducement bacterium source is similar to that of a wild bipolaris maydis population, and the pathogenic ability is reliable. Good and stable pathogen conditions can be provided. The technology is applicable to corn southern leaf blight resistance screening of corn germplasm and also applicable to screening of disease prevention insecticides and technical research.

Description

technical field [0001] The invention relates to the technical field of crop disease research, in particular to a field natural induction technology of corn small spot and the application of the technology. Background technique [0002] Southern corn leaf blight (Southern corn leaf blight) is a worldwide important disease of corn, which mainly damages leaves, and also damages leaf sheaths, bracts, ears and grains. Maize small spot disease can occur throughout the growth period of maize, and the peak period of disease occurrence is before and after tasseling. The diseased leaves spread from the lower part of the plant to the upper part. When the disease is severe, the leaves are covered with lesions, which affect the photosynthesis of the plant and cause the leaves to die early and reduce production. Generally, it can cause a production reduction of more than 10% for susceptible varieties, and a production reduction of 20% to 80% in severe cases. %, or even destroy the crops....

AI Technical Summary

Problems solved by technology

Spray inoculation of corn plants with spores of pathogenic bacteria as the inoculum requires leaf moisturizing treatment to meet the conditions for spore germination and infection, and it is very easy to make all the leaves sprayed with the pathogenic bacteria overly sick, and the growth of the plants is poor in the later stage, showing no resistance. The ability of resistant varieties to resist the vertical expansion of diseases may cause some germplasms that were originally resistant to diseases to become susceptible to diseases and lose their due use value

It is theoretically feasible to collect the crushed diseased leaves from the previous year as an inoculum and apply them to the heart leaves or spread them among the tested corn rows. Due to the influence of many factors, the consistency of each batch of induced bacteria and the reliability of its pathogenicity cannot be guaranteed. However, the artificial inoculation of fresh corn spot disease strains as the initial source of induced bacteria in the field can solve the problem. this question

However, if the fresh diseased plants are stored in the shade for more than half a month, the number of spores and the viability of the spores on the diseased leaf spots will decrease significantly.

Therefore, the selection and utilization of the source of corn spot disease-inducing bacteria in the field will directly affect the occurrence and development of the disease. At present, there is no report on the use of artificially inoculated fresh diseased plants as the field-inducing bacteria source of corn spot spot

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