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Streptomyces A2 strain with strong bacteriostatic effect and application thereof

A technology of bacteriostasis and Streptomyces, applied to Streptomyces A2 strain and its application, biological Streptomyces A2 strain and its application field, can solve the problems of water source and soil microbial flora damage, serious pollution, difficulty, etc. Good development and application prospects, strong antibacterial effect, and small effect on the ecological environment

Active Publication Date: 2021-03-30
ZHONGKAI UNIV OF AGRI & ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Peanut canker sore is a typical soil-borne disease. Controlling it with chemical agents is not only difficult and costly, but also causes serious pollution, causing immeasurable damage to water sources and soil microbial flora.

Method used

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  • Streptomyces A2 strain with strong bacteriostatic effect and application thereof
  • Streptomyces A2 strain with strong bacteriostatic effect and application thereof
  • Streptomyces A2 strain with strong bacteriostatic effect and application thereof

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] The invention provides a technical solution: a strain of Streptomyces A2 Streptomyces sp.A2 with strong antibacterial effect, including the body of Streptomyces A2 Streptomyces sp. Deposited, the deposit number is CCTCCNO: M2016656, and the address of the Chinese Type Culture Collection Center is Wuhan University, Wuhan City, Hubei Province.

[0023] The collection method of the strain is:

[0024] A: The strain was isolated from woodland soil collecte...

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Abstract

The invention discloses a streptomyces A2 strain with a strong bacteriostatic effect and an application thereof, and belongs to the technical field of streptomyces A2 strains and an application thereof. The streptomyces strain A2 is obtained by separation through a conventional dilution plate method, the strain A2 is inoculated into a PD culture medium and cultured for 3 days at the temperature of28 DEG C and the speed of 160 r / min to prepare a seed solution, then the seed solution is inoculated into a liquid fermentation culture medium with the pH value of 7.3, shaking culture is conducted for 7 days at the temperature of 28 DEG C and the speed of 160 r / min, and the biological agent is obtained, which has a high inhibition effect for Sclerotium rolfsii, In addition, the bacterial strainalso has a certain inhibition effect on colletotrichum gloeosporioides, botrytis cinerea and bipolaris maydis. A strain preparation is environment-friendly and non-toxic in source, has small influenceon the ecological environment and low requirements on culture conditions, and has good development and application prospects.

Description

technical field [0001] The invention relates to the technical field of biological streptomyces A2 strain and its application, in particular to a streptomyces A2 strain with strong antibacterial effect and its application. Background technique [0002] Peanut is one of the four major oil crops in my country. The planting area is second only to soybean and rapeseed. my country is also the largest consumer and exporter of peanut. Peanuts are produced in a wide range of areas in my country, mainly concentrated in the North China Plain in the north, the coastal areas of Bohai Bay, the coastal areas of South China and the Sichuan Basin in the south. With the expansion of peanut planting area and the increase of multiple cropping index, the occurrence of peanut diseases has a tendency to increase year by year. According to reports, there are more than 20 kinds of peanut infectious diseases in my country. With the aggravation of diseases, it has seriously affected the production of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCC12N1/20A01N63/00
Inventor 孙辉程东美何俊烺游春平曾永三
Owner ZHONGKAI UNIV OF AGRI & ENG
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