Polyketide synthase PreuA and application thereof in preparation of red powder moss acid

Abstract

The invention relates to a polyketide synthase and an application thereof in preparation of red powder moss acid, and belongs to the technical field of microbial chemistry. According to the invention, the polyketide synthase PreuA related to synthesis of the rubrorubicacid is cloned from the phorbia fungus for the first time, and a yeast mutant strain capable of efficiently producing the rubrorubicacid is constructed based on a saccharomyces cerevisiae heterologous expression technology; and the prepared red powder moss acid is taken as an object, and the inhibitory activity of the red powder moss acid on seven kinds of crop pathogenic fungi is researched. Research finds that the red powder moss acid has different degrees of inhibition effects on four crop pathogenic fungi including apple ring spot bacteria, corn bipolaris maydis, potato verticillium wilt bacteria and sclerotium rolfsii, and has a very strong antagonistic effect (MIC, 25 [mu]g / mL) on the apple ring spot bacteria. The invention greatly enriches the production source of the red powder moss acid, and has important scientific value and application prospect for expanding the derivatization approach of the red powder moss acid and researching and developing novel biopesticides.

Description

technical field [0001] The invention belongs to the technical field of microbiological chemistry, and in particular relates to a polyketide synthase and its application in the preparation of corticoic acid. Background technique [0002] Fungal polyketides ( p oly k etide s , PKs) is a large class of important natural product groups with diverse chemical structures and biological activities, and is a high-quality resource library for the discovery of new drug lead compounds (Keller, 2019). Representative fungal PKs include lovastatin for lowering cholesterol, griseofulvin for fungi, rifamycin for bacteria, mycophenolic acid for immunosuppression, muscle Kinetin and angiogenesis inhibitor cytochalasin E (Hussain et al., 2017). The key enzyme in the biosynthesis of PKs - polyketide synthase ( p oly k etide s ynthase, PKS) has a complex structure and unique catalytic mechanism, which can provide key tools and chemical molecular probes for studying the molecular mechanism ...

AI Technical Summary

Problems solved by technology

But at present, the commercially available carcinic acid (CAS: 480-56-8) is produced by chemical synthesis (Fischer E. & Fischer H., 1913; Koller & Pfeiffer, 1933), the price is about 2000 yuan / mg, the yield is low, and the sales price is low. High price, so its application in food, medicine and other fields is limited

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