Preparation method and use of agricultural bactericide containing parthenolide

A parthenolide, agricultural fungicide technology, applied in the directions of fungicides, botanical equipment and methods, biocides, etc., can solve the problem of less objects for activity research, achieve good inhibitory activity, low equipment requirements, and simple processing Effect

Active Publication Date: 2016-08-03
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that parthenolide has a certain in vitro inhibitory effect on some plant pathogenic fungi, oomycetes, bacteria and even viruses, but there are few objects of research on its activity, and parthenolide has not been used as an agricultural fungicide develop

Method used

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  • Preparation method and use of agricultural bactericide containing parthenolide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 5kg of the whole herb of Pyrita chinensis was extracted three times with 75% ethanol at 60°C to obtain the total extract, which was adsorbed with A8 macroporous resin, eluted with water, 30%, 45%, 60%, and 90% ethanol respectively, and collected Macroporous resin 45%, 60% and 95% eluted parts, concentrated eluate to 1.5kg fluid extract, obtained extract containing parthenolide. The extract is separated and purified through silica gel column chromatography; gel column chromatography and recrystallization (ethyl acetate-methanol) to obtain parthenolide with a purity of more than 90%; 0.5kg surfactant (such as Polysorbate-80) and parthenolide are placed in a homogenizer, heated to 30°C, and 1kg of water is added into the homogenizer while stirring, stirred for 30 minutes, fully mixed, cooled to room temperature, and formed Emulsion in water to obtain parthenolide fungicide.

Embodiment 2

[0022] 10kg of the whole herb of Pyrita chinensis was extracted three times with 95% ethanol at 60°C to obtain the total extract, which was absorbed by D101 macroporous resin, eluted with water, 30%, 45%, and 85% ethanol respectively, and the macroporous resin was collected For 45% and 85% elution sites, concentrate the eluent to 3.0kg liquid extract, which is separated by silica gel column chromatography; gel column chromatography and recrystallization (ethyl acetate-methanol) and other means Purify and obtain parthenolide with a purity of more than 90%; put 0.5kg of surfactant (such as polyoxyethylene nonionic surfactant: OP-10) and parthenolide in a homogenizer and heat to 50°C, while stirring, add 1 kg of water into the homogenizer, stir for 30 minutes, mix well, cool to normal temperature, form water emulsion, and obtain parthenolide fungicide.

Embodiment 3

[0024] The parthenolide fungicide antimicrobial spectrum measurement prepared by embodiment 1:

[0025] The plant pathogenic bacteria tested included Sclerotinias clerotiorum, Fusarium graminearum, Botrytiscinerea, Colletotrichum gloeosporioides, Rhizoctonia solani, and Corn blight ( Helminthosporiummaydis), Botrytiscinerea, Fusariummoniliforme, Rhizoctoniacerealis, Alternariasolani, Verticilliumdahliae, Magnaportheoryzae , Phytophthora capsici, Phytophthorasojae, Xanthomonasoryzaepv.oryzae, Xanthomonasoryzaepv.oryzicola, Xanthomonascampetrispv.camestris, etc. 15 common plant pathogens. All the tested pathogenic bacteria were isolated strains collected in the field.

[0026] The virulence of 15 kinds of phytopathogenic bacteria was initially determined indoors by mycelium growth rate method (fungus, oomycete) or turbidity method (bacteria). Each strain was activated and cultured on PDA (fungus), V8 (oomycete) plate or NB (bacteria) culture medium. For fungi and oomycetes, u...

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Abstract

The invention relates to a botanical pesticide bactericide and a preparation method thereof. The bactericide is obtained through extracting whole herb of Dendranthema lauandulifolium and through processing. Main active components of the bactericide comprise 5-20wt% of parthenolide, water and a surfactant, and are processed to prepare an emulsion in water, and the emulsion in water is used to prevent and control various crop diseases after being diluted. The bactericide has the advantages of easily available raw materials, simple process, low cost, good prevention and control effect on rice bacterial leaf blight, rice bacterial streak, Bipolaris maydis and other diseases, high safety to crops, and no pollution to environment, and is a biological pesticide preparation suitable for Chinese nuisance-free agricultural product production requirements.

Description

technical field [0001] The invention relates to an agricultural fungicide containing parthenolide and a preparation method thereof. Background technique [0002] At present, more than 80% of the pesticides used in China are chemical pesticides. The use of chemical pesticides has greatly promoted the development of modern agriculture. Therefore, research in the field of pollution-free biological pesticide industry and biological control has gained a rare opportunity for development. Using traditional methods to discover and develop a new pesticide requires screening tens of thousands of new compounds or more, which is costly and takes a long time. Isolation and screening of highly active lead compounds from organisms, especially plants, is relatively low cost, highly targeted, and has a short development cycle, and the developed pesticides are environmentally friendly, relatively safe to non-target organisms, have specific modes of action, and are not easy to produce. With ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N25/04A01P3/00
CPCA01N25/04A01N43/90A01N2300/00
Inventor 徐曙王奇志冯煦王碧刘飞陈雨单宇印敏管福琴王鸣赵友谊张建华
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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