Natural biological preserving agent for aquatic products and preparation method and application thereof

A technology of biological preservatives and aquatic products, which is applied in the direction of food ingredients as antimicrobial preservation, application, food preservation, etc. It can solve the problems of unsatisfactory preservation effects of biological preservatives, prevent breeding and reproduction, increase output value, and reduce cost effect

Inactive Publication Date: 2016-08-17
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Compared with the traditional fresh-keeping agents for aquatic products, the existing biological preservatives have significant

Method used

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  • Natural biological preserving agent for aquatic products and preparation method and application thereof
  • Natural biological preserving agent for aquatic products and preparation method and application thereof
  • Natural biological preserving agent for aquatic products and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the preparation of aquatic product natural biological preservative

[0041] The natural biological antistaling agent for aquatic products in the present embodiment is prepared through the following steps:

[0042] 1. Preparation of chondroitin sulfate oligosaccharides

[0043] (1) Using tuna bones and squid throat bones as raw materials, boil the raw materials for 1 hour, remove residual muscle, fat and other connective tissues, and homogenize with a high-speed tissue grinder to obtain a homogenate;

[0044] (2) Add acetone to the above-mentioned homogenate according to the volume ratio of 1:1, stir and degrease at 150rpm for 2h, let stand, discard the acetone layer, and obtain a degreased solid;

[0045] (3) Add 0.5 mol / L phosphate buffer to the above-mentioned degreased solid at a solid-to-liquid ratio of 1:1 g / mL, adjust the pH to 5.0, and stir evenly to obtain a mixture;

[0046] (4) Heat the above mixture to 50°C, add bromelain, the amount of enzyme...

Embodiment 2

[0065] Embodiment 2: the preparation of aquatic product natural biological preservative

[0066] The natural biological antistaling agent for aquatic products in the present embodiment is prepared through the following steps:

[0067] 1. Preparation of chondroitin sulfate oligosaccharides

[0068] (1) Using tuna bones and squid throat bones as raw materials, boil the raw materials for 1 hour, remove residual muscle, fat and other connective tissues, and homogenize with a high-speed tissue grinder to obtain a homogenate;

[0069] (2) Add acetone to the above homogenate according to the volume ratio of 1:2, stir and degrease at 150rpm for 3h, let stand, discard the acetone layer to obtain a degreased solid;

[0070] (3) Add 0.5 mol / L phosphate buffer to the above-mentioned degreased solid at a solid-to-liquid ratio of 1:2 g / mL, adjust the pH to 6.0, and stir evenly to obtain a mixture;

[0071] (4) Heat the above mixture to 60°C, add bromelain, the amount of enzyme added is 15...

Embodiment 3

[0090] Embodiment 3: the preparation of aquatic product natural biological preservative

[0091] The natural biological antistaling agent for aquatic products in the present embodiment is prepared through the following steps:

[0092] 1. Preparation of chondroitin sulfate oligosaccharides

[0093] (1) Using tuna bones and squid throat bones as raw materials, boil the raw materials for 1 hour, remove residual muscle, fat and other connective tissues, and homogenize with a high-speed tissue grinder to obtain a homogenate;

[0094] (2) Add acetone to the above-mentioned homogenate according to the volume ratio of 1:1, stir and degrease at 150rpm for 2h, let stand, discard the acetone layer, and obtain a degreased solid;

[0095] (3) Add 0.5 mol / L phosphate buffer to the above-mentioned degreased solid at a solid-to-liquid ratio of 1:3 g / mL, adjust the pH to 7.0, and stir evenly to obtain a mixture;

[0096] (4) Heat the above mixture to 50°C, add bromelain, the amount of enzyme...

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Abstract

The invention relates to a natural biological preserving agent for aquatic products, which is prepared from the following ingredients in parts by weight: 16 to 24 parts of chondroitin sulfate oligosaccharide, 35 to 40 parts of antioxidative peptide, 41 to 45 parts of curcuma extract and 300 to 450 parts of sterilized water. The invention further relates to a preparation method of the biological preserving agent and application to preservation of the aquatic products. The biological preserving agent disclosed by the invention is non-toxic, has no residues, is low in cost, can effectively avoid oxidative damage to fat, proteins and the like of the aquatic products, and keeps the bright color of the aquatic products; meanwhile, the broad-spectrum antibacterial activity of the curcuma extract is sufficiently utilized to prevent breeding and reproduction of various common bacteria of the aquatic products in the preserving process of the aquatic products, so that the natural biological preserving agent takes an effective preserving effect on the aquatic products and a preservation period of the aquatic products is obviously prolonged. Moreover, in the preparing process of the preserving agent, both the chondroitin sulfate oligosaccharide and the antioxidative peptide use aquatic product processing byproducts as raw materials, so that production cost can be reduced, the output value of aquatic product processing is improved, the environment is improved, and the natural biological preserving agent has obvious social effects.

Description

technical field [0001] The invention relates to the technical field of fresh-keeping of aquatic products, in particular to a natural biological fresh-keeping agent for aquatic products and its preparation method and application. Background technique [0002] Aquatic products are more and more popular because of their delicious taste, rich nutrition, and rich in n-3 unsaturated fatty acids and other active substances that have curative effects on cardiovascular diseases. Because the water content in the muscles of fish and shrimp and other aquatic products is about 70%, the tissue is fragile, the natural immune substances are less, the unsaturated fatty acids are easily oxidized, and the soluble protein content is high, so it is not easy to store compared with ordinary animal meat tissues, and it is easy to reduce the quality and even spoil. . [0003] Antistaling agent treatment is a common method in the storage and transportation of aquatic products. It can not only effect...

Claims

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Application Information

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IPC IPC(8): A23B4/20A23L3/3562A23L3/3472A23L3/3481
CPCA23B4/20A23L3/3472A23L3/3481A23L3/3562A23V2002/00A23V2200/10A23V2250/2112A23V2250/2042A23V2250/55A23V2250/60
Inventor 王进波齐莉莉梅乐和胡升喻鹏飞
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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