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Method for synchronously extracting grease and antioxidative peptide from soybeans

A technology for synchronous extraction of antioxidant peptides, which is applied in fat oil/fat production, fat production, fermentation, etc., can solve the problems of long time consumption, low yield, high cost, etc., and achieve safe operation, simple equipment, and high added value Effect

Inactive Publication Date: 2017-05-31
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of antioxidant soybean peptides lacks a technical basis. The extraction of antioxidant peptides takes a long time, the yield is low, the effect is poor, and the cost is high. As a result, the soybean peptide products on the market are currently in the primary development stage.

Method used

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  • Method for synchronously extracting grease and antioxidative peptide from soybeans
  • Method for synchronously extracting grease and antioxidative peptide from soybeans
  • Method for synchronously extracting grease and antioxidative peptide from soybeans

Examples

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Embodiment 1

[0020] Specific steps for the determination of the antioxidant polypeptide reducing ability: dissolve 10 mg of the sample in 1 mL of distilled water, add 2.5 mL of 0.2 mol / L PBS (pH 6.6) and 2.5 mL of 1% potassium ferricyanide solution and mix well, and mix well from the above Take 1 mL of the mixed solution, then add 0.2 mL of 0.1% ferric chloride solution and mix well, then add 1 mL of distilled water and shake well, adjust to zero with distilled water, and measure the absorbance at 700 nm. The higher the absorbance, the stronger the reducing ability of the sample.

[0021] attached by figure 1 It can be seen that the reducing ability of soybean protein enzymatic hydrolyzate is greater than that of unenzymolyzed soybean protein, and the reducing ability of enzymatic hydrolyzate reaches the maximum at 4h, the former is 1.91 times of the latter. After 4 hours of enzymatic hydrolysis, the reducing ability of the enzymatic hydrolyzate gradually decreased. The reason may be that...

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Abstract

The invention provides a method for synchronously extracting grease and antioxidative peptide from soybeans and belongs to the food processing technology. The method comprises steps as follows: (1), the soybeans are smashed and sieved, water is added for soaking, and a semi-solid mixed solution is formed; (2), the semi-solid mixed solution is pretreated with a high-voltage pulse electric field; (3), three-enzyme compounds of Protex-7L neutral protease, alkaline protease and bromelain are added to the mixed solution after being subjected to pulse electric field treatment for enzymolysis; (4), the mixed solution after enzymolysis is cooled to 4 DEG C, and soybean grease and hydrolysate are obtained through centrifugation and separation; (5), the hydrolysate is taken and subjected to closure separation by a 10kDa ultrafiltration membrane (a PP-100 hollow-fiber module), and a soybean peptide solution with molecular weight of 10 kDa or lower is obtained; (6), the soybean peptide solution is subjected to vacuum freeze drying, and the antioxidative soybean peptide with high added value can be directly obtained.

Description

technical field [0001] The invention belongs to food processing technology, and mainly relates to a method for synchronously extracting oil and antioxidant polypeptide from soybeans. Background technique [0002] At present, vegetable oil is produced mainly by leaching and mechanical pressing. The pressing method has simple process, less supporting equipment, strong adaptability to oil varieties, and pure flavor, but the cake after pressing has a high residual oil content, low oil extraction efficiency, large power consumption, and easy loss of parts. Although the leaching method has a high oil extraction rate (95%-98%), the protein denaturation of the wet meal is serious during the high temperature desolventization process, and the use of organic solvents increases the complexity of the process, reduces the safety of production, and causes environmental pollution. , and with the depletion of petroleum resources, organic solvents will also be depleted. Aqueous enzymatic oi...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C12P21/06
Inventor 李杨隋晓楠寻崇荣江连洲齐宝坤丁俭王中江朱颖
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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