Anti-titanium oxide from collagen and its use

A technology of collagen and antioxidant peptides, applied in peptide/protein components, medical preparations containing active ingredients, peptides, etc., can solve the problem of losing antioxidant activity

Inactive Publication Date: 2007-10-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the relatively large molecular weight of the amino acid residues of natural antioxidants (antioxidant peptides) separated in the prior art, which are easily further hydrolyzed by pepsin, trypsin and small intestinal protease in

Method used

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  • Anti-titanium oxide from collagen and its use
  • Anti-titanium oxide from collagen and its use
  • Anti-titanium oxide from collagen and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Test of Antioxidative Ability of Antioxidant Peptide Mixture Obtained by Hydrolyzing Collagen with Protease At 37°C, the collagen powder of pigskin was mixed with purified water so that the weight percentage of collagen in the mixture was 5wt %, adjust the pH value of collagen to 2.0 with 6N hydrochloric acid, then add pepsin (pepsin) (the weight ratio of collagen and protease is 2500: 1, carry out hydrolysis reaction 24 hours; ℃ for 5 minutes, and then boil for another 3 minutes to stop the enzymatic hydrolysis reaction.

[0062] Then, continue to carry out mixed enzymolysis with pronase and bovine trypsin on this basis: adjust the pH value of above-mentioned pepsin hydrolyzate to be 7.5, enzyme (the weight ratio of pronase and bovine trypsin is 1: 1) and substrate The ratio is 2 / 125, the hydrolysis temperature is 37°C, and the hydrolysis time is 24 hours. At the end of the hydrolysis, the hydrolyzate is boiled for 3 minutes to inactivate the enzyme, and a p...

Embodiment 2

[0065] Example 2, further separation and identification of 4 antioxidant peptides of the present invention

[0066] Dissolve the lyophilized powder obtained in Example 1 with a small amount of deionized water, and separate it with a gel filtration chromatographic column SephadexLH-20 (2.5×80cm), using deionized water as the mobile phase, and the peptides in the mixture are eluted sequentially , detect the concentration of peptide at 254nm, as shown in Figure 1 (A), measure the antioxidant activity of the eluent corresponding to each absorption peak, as shown in Figure 1 (B), collect the antioxidant activity higher than 50% Component P4, then lyophilized into powder with a lyophilizer

[0067] The lyophilized powder of component P4 was dissolved with 0.5M tris-HCl buffer solution (pH 8.3), and loaded onto the DEAE-Sephadex A-25 (Cl-type, Sigma, 2.0×35cm) chromatogram equilibrated with the buffer solution Separation and purification on the column. Use 0.5M tris-HCl buffer to e...

Embodiment 3

[0070] Component D was separated and purified by the same method. Two antioxidant active components were separated. The amino acid sequence of the peptide was identified by tandem chromatography (MS / MS) and amino acid sequence analyzer, and the amino acid sequence of the antioxidant peptide was identified as Leu-Gln-Gly-Met (molecular weight 447.2) and Leu-Gln-Gly-Met -Hyp (molecular weight 560.3). Embodiment 3, use antioxidant peptide of the present invention to add in soy sauce as antioxidant

[0071] Soy sauce production formula (weight ratio) is as follows:

[0072] A Raw soy sauce, 5%, main ingredient;

[0073] B Caramel pigment, according to production needs (about 0.05%);

[0074] C Monosodium glutamate and [I+G], the two are mixed at a weight ratio of 35-45:1, 0.6%-0.7%;

[0075] D Collagen-derived antioxidant peptide, added 0.5%, can be used as a nutritional enhancer and freshness aid at the same time, increasing the amino acid content of the product and increasing...

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Abstract

This invention relates to a series of antioxygen peptide stem from collagen protein. It possess one of as follows amino acid sequence: ( 1) Hyl - Cys; ( 2) Gln - Gly - Ala - Arg; ( 3) Leu - Gln - Gly - Met; ( 4)) Leu - Gln - Gly - Met - Hyp. This antioxygen peptide could synthesize through polypeptide instrument chemical process, as well as through hydrolization of collagen protein, and then obtain by separating and purifying acquired hydrolysate. The antioxygenation of this antioxygen peptide is very strong, not only could substitute BHT to use as edible additive, furthermore could as activating functional ingredient. This product could be used in foodstuff, health products, makeup, even animal foodstuff and feedstuff, has abroad field of application and notable social benefit and economic benefit. The antioxygen peptide only has two to five amino acid, molecular weight smallness, more easily directly assimilate by alimentary tract.

Description

technical field [0001] The present invention relates to a series of antioxidant peptides derived from collagen and their use as antioxidants. Background technique [0002] Chemically synthesized antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-p-cresol (BHT) are usually used as food additives in the food industry to prevent food from oxidative discoloration or caused by oxygen. quality deterioration. However, the application of these synthetic antioxidants in food is increasingly limited due to the potential hazards to health. In the last two decades, people have turned their attention to natural and safe antioxidants from plants and animals. Natural antioxidants can not only prevent the oxidation of the oil system in food, but also prevent the damage of free radicals in the human body, and delay various degenerative diseases caused by aging, such as cancer, coronary heart disease, diabetes and so on. Some reports have found that protein hydrolyzate h...

Claims

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Application Information

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IPC IPC(8): C07K5/06C07K5/10C07K7/06C07K1/00C07K1/12A61K38/05A61K38/07A61K38/08A61K8/64A61P39/06A23K1/16A23K20/147
Inventor 李博籍保平陈峰
Owner CHINA AGRI UNIV
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