Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof

Abstract

The invention relates to a pseudosciaena crocea flesh antioxidative peptide and use thereof, and the pseudosciaena crocea flesh antioxidative peptide is characterized in that: the amino acid sequence is Tyr-Leu-Met-Ser-Arg, and the molecular weight is 651.77Da ((M + H) + 652.45Da) given by ESI (electronic spray ion)-MS (mass spectrum) detection. The preparation method of the antioxidative peptide includes homogenizing, enzymolysis, ultrafiltration and chromatography and other steps. The prepared antioxidative peptide Tyr-Leu-Met-Ser-Arg has good scavenging effect on DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, hydroxyl radical, ABTS radical and superoxide anion free radical; at the same time, the Tyr-Leu-Met-Ser-Arg also shows good lipid peroxidation inhibition effect. The Tyr-Leu-Met-Ser-Arg has significant antioxidant activity, and can be used as medicines, health food or food additives.

Description

technical field [0001] The invention relates to a biologically active peptide and its preparation method and use, in particular to a large yellow croaker fish meat antioxidant peptide and its preparation method and use. Background technique [0002] large yellow croaker ( Pseudosciaena crocea ), also known as yellow croaker and large yellow croaker, is one of the traditional "four seafood" (large yellow croaker, small yellow croaker, hairtail, squid) in China, and is a valuable economic fish in my country's coastal waters. However, due to overfishing, wild resources have been severely damaged. In recent years, with the gradual maturity of artificial breeding technology, various farming models such as cages, seines and earthen ponds have been applied in large yellow croaker farming. The output of large yellow croaker has increased year by year, and the deep processing technology of large yellow croaker has received increasing attention. [0003] However, the applicant found...

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Problems solved by technology

[0003] However, the applicant found that the preparation of antioxidant peptides from large yellow croaker meat by enzymatic hydrolysis technology using large yellow croaker meat as raw material is still at a blank stage, and the preparation and application of highly active fish antioxidant peptides using enzymatic hydrolysis products as materials is even more important. No report

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