Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof

A technology of antioxidant peptides and large yellow croaker, which is applied in peptide preparation methods, chemical instruments and methods, food preparation, etc., can solve the problems of high activity fish antioxidant peptides that have not been reported, and achieve safety, non-toxic side effects, and easy digestion Effect of absorption, strong antioxidant activity

Active Publication Date: 2014-12-31
ZHEJIANG OCEAN UNIV
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  • Claims
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Problems solved by technology

[0003] However, the applicant found that the preparation of antioxidant peptides from large yellow croaker meat by enzymatic hydrolysis technology using large yellow croaker meat as raw mat...

Method used

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  • Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof
  • Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof
  • Pseudosciaena crocea flesh antioxidative peptide and preparation method and use thereof

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Embodiment 1

[0027] Example 1: A preparation method of large yellow croaker meat antioxidant peptide, the preparation process is as follows: large yellow croaker meat "enzymolysis" enzymatic hydrolyzate "ultrafiltration" ion exchange chromatography "gel filtration chromatography" high performance liquid chromatography Preparation of "Antioxidant Peptides.

[0028] 1) Mince the large yellow croaker meat, add distilled water according to the ratio of material to liquid 1 g:12 mL, adjust the pH value to 7.0, and keep warm at 50 °C for 10 min.

[0029] 2) Add papain according to 1.0% of the mass of large yellow croaker meat, and enzymolyze at 50°C for 4 hours; adjust the pH value of the above enzymatic solution to 9.5, add alkaline protease according to 2.0% of the mass of large yellow croaker flesh, and enzymatically digest at 50°C Solution 4 h. After repeated experiments, the enzymatic hydrolysis product has stable components and high repeatability.

[0030] 3) Heat the enzymatic hydrolysi...

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Abstract

The invention relates to a pseudosciaena crocea flesh antioxidative peptide and use thereof, and the pseudosciaena crocea flesh antioxidative peptide is characterized in that: the amino acid sequence is Tyr-Leu-Met-Ser-Arg, and the molecular weight is 651.77Da ((M + H) + 652.45Da) given by ESI (electronic spray ion)-MS (mass spectrum) detection. The preparation method of the antioxidative peptide includes homogenizing, enzymolysis, ultrafiltration and chromatography and other steps. The prepared antioxidative peptide Tyr-Leu-Met-Ser-Arg has good scavenging effect on DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, hydroxyl radical, ABTS radical and superoxide anion free radical; at the same time, the Tyr-Leu-Met-Ser-Arg also shows good lipid peroxidation inhibition effect. The Tyr-Leu-Met-Ser-Arg has significant antioxidant activity, and can be used as medicines, health food or food additives.

Description

technical field [0001] The invention relates to a biologically active peptide and its preparation method and use, in particular to a large yellow croaker fish meat antioxidant peptide and its preparation method and use. Background technique [0002] large yellow croaker ( Pseudosciaena crocea ), also known as yellow croaker and large yellow croaker, is one of the traditional "four seafood" (large yellow croaker, small yellow croaker, hairtail, squid) in China, and is a valuable economic fish in my country's coastal waters. However, due to overfishing, wild resources have been severely damaged. In recent years, with the gradual maturity of artificial breeding technology, various farming models such as cages, seines and earthen ponds have been applied in large yellow croaker farming. The output of large yellow croaker has increased year by year, and the deep processing technology of large yellow croaker has received increasing attention. [0003] However, the applicant found...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/18A61K38/08A61P39/06A23L1/305A23L33/18
Inventor 王斌马路凯迟长凤李涛
Owner ZHEJIANG OCEAN UNIV
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