Antioxydizing peptide mixture from collagen and its preparation process and use

A collagen and antioxidant peptide technology, applied in chemical instruments and methods, compositions for inhibiting chemical changes, non-active components of polymer compounds, etc. Activity and other issues, to achieve the effect of strong antioxidant effect, significant social and economic benefits, and small molecular weight

Inactive Publication Date: 2007-07-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the prior art when preparing natural antioxidants, or the sources of raw materials are very limited, which is unfavorable for wide use; or the cost of equipment is high; or the molecular weight of the isolated antioxidant peptide is relatively large, which is diffic

Method used

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  • Antioxydizing peptide mixture from collagen and its preparation process and use
  • Antioxydizing peptide mixture from collagen and its preparation process and use
  • Antioxydizing peptide mixture from collagen and its preparation process and use

Examples

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Effect test

Embodiment 1

[0047] Example 1, using pepsin and pronase to hydrolyze collagen to prepare the antioxidant peptide mixture derived from collagen of the present invention

[0048] 1) At 37°C, mix the collagen powder of pigskin with purified water so that the weight percentage of the collagen in the mixed solution is 5wt%, adjust the pH value of the collagen to 2.0 with 6N hydrochloric acid, then add pepsin ( pepsin) (the weight ratio of collagen and protease is 2500: 1), carry out hydrolysis reaction for 24 hours, when hydrolysis finishes, hydrolyzate is heated at 90 ℃ for 5 minutes, then boil and keep for 3 minutes again, stop enzymolysis reaction.

[0049] At the same hydrolysis temperature, the pepsin hydrolyzate was further hydrolyzed with pronase E. First use sodium hydroxide to adjust the pH value of the pepsin hydrolyzate to 7.5, then add pronase E (pronase E) (the weight ratio of pepsin hydrolyzate and protease is 125: 1), carry out the hydrolysis reaction for 24 hours, and the hydrol...

Embodiment 2

[0053] Example 2, using pepsin, papain and bovine trypsin to hydrolyze collagen to prepare the antioxidant peptide mixture derived from collagen of the present invention

[0054] 1) At 37°C, mix the collagen powder of pigskin with deionized water so that the weight percentage of the collagen in the mixture is 7wt%, adjust the pH value of the collagen to 2.0 with hydrochloric acid, and then add pepsin ) (the weight ratio of collagen and protease is 2500: 1), carry out the hydrolysis reaction for 24 hours, when the hydrolysis ends, the hydrolyzate is heated at 90° C. for 5 minutes, then boiled and then kept for 3 minutes to stop the enzymolysis reaction.

[0055] The pepsin hydrolyzate was then hydrolyzed with papain. Adjust the pH value of the above-mentioned pepsin hydrolyzate to 6.2, then add papain (papain), the weight ratio of papain and pepsin hydrolyzate is 1: 50, carry out the hydrolysis reaction at 25 ℃ for 24 hours, when the hydrolysis ends, the hydrolyzate After boil...

Embodiment 3

[0060] Example 3, using pepsin and bovine trypsin to hydrolyze collagen to prepare the antioxidant peptide mixture derived from collagen of the present invention

[0061] 1) At 37°C, mix the collagen powder of fish skin with purified water so that the weight percent of collagen in the mixed solution is 2wt%, adjust the pH value of collagen to 2.0 with 6N hydrochloric acid, then add pepsin ( pepsin) (the weight ratio of collagen and protease is 2500: 1), carry out hydrolysis reaction for 24 hours, when hydrolysis finishes, hydrolyzate is heated at 90 ℃ for 5 minutes, then boil and keep for 3 minutes again, stop enzymolysis reaction.

[0062] The pepsin hydrolyzate was then hydrolyzed with protease from bovine pancreas. Adjust the pH value of the above-mentioned pepsin hydrolyzate to 7.5, then add bovine pancreas protease (protease from bovine pancreas), the weight ratio of bovine pancreas protease and pepsin hydrolyzate is 1:125, carry out the hydrolysis reaction at 37 ° C for ...

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Abstract

The present invention is antioxidant peptide mixture obtained through hydrolyzing collagen and its preparation process. The preparation process includes hydrolyzing collagen with protease to obtain coarse product of antioxidant peptide mixture; precipitating or filtering to obtain hydrolysate with fat, macro molecular protein, polypeptide and impurity eliminated; and final separating purification and concentration to obtain the antioxidant peptide mixture. The present invention has wide material source and low cost, and the product has powerful antioxidant effect, and may be used as food additive as well as in cosmetics, health product, feed, etc.

Description

technical field [0001] The present invention relates to a mixture of antioxidant peptides derived from collagen, its preparation method, and its use as antioxidant. Background technique [0002] Chemically synthesized antioxidants such as butyl hydroxyanisole (BHA) and 2.6-di-tert-butyl-p-cresol (BHT) are usually used as food additives in the food industry to prevent food from oxidative discoloration or quality caused by oxygen. deterioration. However, the application of these synthetic antioxidants in food is increasingly limited due to the potential hazards to health. In the last two decades, people have turned their attention to natural and safe antioxidants from plants and animals. Natural antioxidants can not only prevent the oxidation of the oil system in food, but also prevent the damage of free radicals in the human body, and delay various degenerative diseases caused by aging, such as cancer, coronary heart disease, diabetes and so on. Some reports have found tha...

Claims

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Application Information

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IPC IPC(8): C12P21/02C09K15/20A23L3/3526A23L1/305A23K1/16A61K47/42A61K38/01A61P39/06A23K20/147A23L33/18
Inventor 李博籍保平陈峰
Owner CHINA AGRI UNIV
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