Method for evaluating anti-aging efficacy of cosmetics

Abstract

The invention belongs to the technical field of detection. The invention discloses a method for evaluating the anti-aging efficacy of cosmetics. According to the method, indexes such as a beta-galactosidase activity inhibition effect, a telomerase activity enhancement effect, an oxygen free radical elimination effect, a lipid browning content, a survival rate and the like are evaluated. Accordingto the invention, experimental operation and observation are facilitated, and since chemical substances are directly absorbed into a circulating system, and painful and time-consuming injection or invasive surgery is not needed, so that pressure or tissue damage related to a rodent aging model is avoided. Zebra fish is equivalent to mice for 8-10 days in one day, the experiment period is shorter,and the cost is low; The zebra fish, as a complete living body model, can be used for high-throughput screening of drugs, especially screening of chemical drugs. Embryos and juvenile fishes are transparent, various tissue and organ changes can be directly observed under a microscope, and experiment results are visual and easy to understand.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for evaluating the anti-aging efficacy of cosmetics. Background technique [0002] Most of the existing aging screening models use rodents (such as mice, rabbits, and dogs) as model animals. The aging models can show aging phenotypes similar to humans, and their underlying biochemical mechanisms are conserved with humans. Includes spontaneous, induced, and transgenic models. Spontaneous aging models include natural aging and rapid aging models. The natural aging model is most in line with the characteristics of human aging, mainly manifested in the decline of anti-stress ability, immune function, and learning and memory functions. It omits the steps of surgery or drug administration, and is a good way to study the aging mechanism It is an ideal model, but the characteristics of long feeding period, poor health, high mortality and large individual differences also limi...

AI Technical Summary

Problems solved by technology

[0008]1. The characteristics of the natural aging model, such as long breeding period, poor health, high mortality rate and large individual differences, also limit its wide application;

[0009]2. The non-human primate model has larger body size, tight source, and longer life span, while the pathological manifestations of aging or aging-related diseases usually appear after middle age, Therefore, the experimental research period is long, and it takes a lot of manpower and time, which increases the difficulty of carrying out experimental research;

[0010]3. Invertebrate models have differences in physiological structure and functional activity and lack of important pathological mechanisms, so the research results are difficult to verify;

[0011]4. Due to the high cost of raising mammals and the long development cycle, it is impossible to evaluate multiple test products at the same time

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