Crispr effector system based diagnostics

Abstract

The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA withcomparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried formatfor convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

Description

[0001] Statement Regarding Federally Funded Research

[0002] This invention was made with government support under grant numbers MH100706 and MH110049 awarded by the National Institutes of Health and grant number HDTRA1-14-1-0006 awarded by the Defense Threat Reduction Agency. The government has certain rights in this invention. technical field

[0003] The subject matter disclosed herein generally relates to rapid diagnostics associated with the use of CRISPR effector systems. Background technique

[0004] Nucleic acid is a universal symbol of biological information. The ability to rapidly detect nucleic acids with high sensitivity and single-base specificity on a portable platform has the potential to revolutionize the diagnosis and monitoring of many diseases, provide valuable epidemiological information, and serve as a universal scientific tool. Although many methods for detecting nucleic acids have been developed (Du et al., 2017; Green et al., 2014; Kumar et al., 2...

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