Human papillomavirus HPV DNA fragment, specific primer and application thereof

A human papillomavirus-specific technology, applied in the field of detection and/or typing of human papillomavirus DNA, can solve problems such as inability to do typing
CN101851630AInactive Publication Date: 2010-10-06白向阳 +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
白向阳
Publication Date
2010-10-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a human papillomavirus HPV DNA fragment, a mix primer for human papillomavirus HPV DNA detection and / or parting and application thereof. The mixed primer can specifically amplify to obtain one or a plurality of DNA sequences from HPV6, HPV 11, HPV 16, HPV 18, HPV 26, HPV 31, HPV 33, HPV 35, HPV 39, HPV 40, HPV 42, HPV 43, HPV 44, HPV45, HPV 51, HPV 52, HPV 53, HPV 54, HPV 55, HPV 56, HPV 58, HPV 59, HPV 61, HPV 66, HPV 68, HPV 70, HPV 72, HPV 73, HPV 81, HPV 82 and HPV 83, can be used for the human papillomavirus HPV DNA detection and / or parting and prepared into a PCR kit. The invention can detect common types of HPV at a time, greatly improve the HPV detection efficiency, overcome the defect of missed detection of latent infection by a serology and IHC detection method, realize early diagnosis of HPV diseases, and is suitable for clinical HPV gene detection and parting and guides prevention and cure of cervical carcinoma.
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Description

technical field

[0001] The invention belongs to biotechnology and relates to the detection and / or typing of human papillomavirus DNA. Background technique

[0002] Human papilloma virus (HPV) is a small DNA double-stranded virus. More than 100 HPV DNAs have been isolated, and more than 40 of them are related to cervical infection and lesions. According to the pathogenicity of HPV, it is divided into high-risk type and low-risk type. The low-risk type mainly causes exophytic condyloma lesions, flat condyloma lesions and low-grade cervical intraepithelial lesions in the genital tract, perianal skin and lower vagina. Neoplastic change (CIN I); the high-risk type mainly leads to the occurrence of CIN II and III grade lesions and cervical cancer. Persistent high-risk HPV infection is an inevitable process for CIN to develop into cervical cancer. The time interval from infection with high-risk HPV to development of cervical cancer is 15 years. Therefore, cervical cancer is a spec...

Claims

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