9results about How to "Precision editing" patented technology

The application provides a three-dimensional face editing and system based on neural radiance field decoupling, which performs geometric editing on a three-dimensional face through a semantic mask according to random noise, performs three-dimensional consistent style migration on a material in a case where a reference image is given, and finally outputs a three-dimensional face that can be locally geometrically edited and overall style edited, so as to solve the problems of low generation quality, difficult training, difficult geometric control, difficult material decoupling control and ineffective style migration of the prior art.

The present application relates to the field of genetic engineering, in particular to a pig cell model of NF2 gene mutation and a construction method and application thereof. The construction method comprises the following steps: 1) designing and synthesizing pegRNA and Nick sgRNA according to the pig NF2 gene; 2) constructing a vector: taking an sgRNA expression plasmid as a skeleton, inserting pegRNA into a BbsI enzyme cutting site of the plasmid to construct a pCL108PE1 vector; taking the sgRNA expression plasmid as the skeleton, inserting Nick sgRNA into the BbsI enzyme cutting site of the plasmid to construct a pCL108NICK1 vector; 3) electrotransferring cells: simultaneously electrotransferring PE protease plasmid, the pCL108PE1 vector and the pCL108NICK1 vector into pig cells; 4) obtaining the pig cell model of NF2 gene mutation: culturing the electrotransferred pig cells, screening out positive clone cells and obtaining the pig cell model of NF2 gene mutation.

This application presents a gRNA probe library, its preparation and design methods, a method for targeting genes, and a nucleic acid imaging method. This application combines gRNA probes containing modified groups with nucleases, enabling dynamic observation of DNA and RNA by binding to target genomic sequences or RNA transcripts in living cells. By utilizing fluorescently labeled crRNAs, tracrRNAs, and sgRNAs, this application achieves real-time imaging of repetitive and non-repetitive sites, establishing a powerful gene imaging toolkit. Furthermore, the gRNA probe library prepared in this application will be applied to CRISPR systems for gene editing, transcriptional regulation, gene targeting, and delivery.

The invention relates to the technical field of image inversion, and provides a diffusion probability model-based image inversion method, which comprises the following steps of: firstly, carrying out noise addition on a real image based on a deterministic noise addition algorithm of a denoising diffusion implicit model to generate an implicit vector; secondly, regarding a text embedding vector generated by corresponding text description as an optimizable variable; then, inputting the text embedded vector and the implicit vector into a stable diffusion model together, and performing a denoising process based on a denoising algorithm of a denoising diffusion implicit model to obtain a reconstructed image; and finally, adopting a root-mean-square error between the reconstructed image and the real image as an optimization loss function, and optimizing a text embedding vector through a gradient descent algorithm so as to realize high-precision reconstruction of the real image.

This disclosure relates to a cleaning control method, apparatus, device, medium, and program product for a robotic vacuum cleaner. According to an example of this disclosure, the method includes: displaying an initial work area map in a user interface; in response to receiving a shape editing instruction for an ultrasonic carpet area, editing the shape of the ultrasonic carpet area according to the editing method indicated by the shape editing instruction to obtain a target work area map; and sending the target work area map to the robotic vacuum cleaner for cleaning based on the target work area map. This disclosure enables flexible and accurate editing of the shape of the ultrasonic carpet area in the robotic vacuum cleaner's work area map according to user-triggered shape editing instructions, improving the accuracy of the ultrasonic carpet shape and thus enhancing the subsequent cleaning effect of the robotic vacuum cleaner.

The present invention provides a sgRNA for targeted knockout of bovine mammary epithelial cell TARDBP gene and a cell line constructed thereof. The sgRNA includes sgRNA1 and sgRNA2, and the nucleotide sequences of the sgRNA are shown in SEQ ID NO:1 and SEQ ID NO:2, respectively. The sgRNA of the present invention can accurately guide the Cas9 protein to target and cut the bovine mammary epithelial cell TARDBP gene, and the expression vector cell conversion rate obtained by constructing the sgRNA is high, which greatly improves the success rate of knocking out the bovine mammary epithelial cell TARDBP gene, and obtains the TARDBP gene knockout mammary epithelial cell line. In order to facilitate the study of the function and mechanism of the TARDBP gene in milk fat secretion and milk production of dairy cows, a cell model is provided, which lays the foundation for dairy cow biological breeding.

The application provides a T cell receptor and application thereof, and particularly relates to a T cell receptor (TCR) capable of specifically recognizing a glioblastoma-associated antigen and application thereof, wherein the glioblastoma-associated antigen can form a complex with HLA-A*0201 and be presented to the surface of a glioblastoma cell. The T cell of a patient is reformed into a cytotoxic T cell (TCR-T) specific to the GB-associated antigen. When the gene-edited TCR-T cell is input into the patient, the TCR-T cell specific to the GB-associated antigen is activated by specific recognition when encountering the antigen complex on the tumor cell, thereby expanding in the patient and achieving the effect of treating the tumor by killing the tumor cell. The application provides a new way for treating glioblastoma.

The present application relates to the technical field of genetic engineering, and more particularly to a super mini gene editor and application thereof. The present application first performs an engineering operation of simplifying the functional domain of a TnpB editor, and systematically develops a new super mini editor of 380-400 AA. The super mini new editor of the present application can efficiently realize precise and efficient editing from mammalian cells to living animals, and has great application value.

The invention relates to the technical field of dental medicine, in particular to a buccal width editing method and device of a dental model, equipment and a storage medium. The method comprises the following steps: acquiring a tooth model; the tooth model is initialized, target data of the tooth model are determined, and the target data comprise an initial buccal width feature value of the tooth model; determining a target buccal width characteristic value input by the user; constructing a deformation parameter according to the target data and the target buccal width characteristic value; and according to the deformation parameters, performing deformation processing on the tooth model through a grid-based Laplace deformation method. According to the method, an initial buccal width characteristic value of a tooth model is calculated, and a target buccal width characteristic value input by a user in real time is combined to construct a deformation parameter of Laplacian transformation, that is, the tooth model conforming to the target buccal width characteristic value is generated in a grid-based Laplacian transformation mode; accurate and fast editing of the buccal width features of the tooth model is realized.