66results about How to "Precision editing" patented technology

A system and method for automatically georeferencing digitized images selects and analyzes image landmark points from disparate sources for image co-registration. A composite alignment of raster images and geographic target vectors is automatically generated using metadata sources for use in geographic display and editing environments. Cartographic alignment of raster images to geographic vector data sets is performed to co-position image data with geographic vector data sets. The co-registration and display permits a meaningful overlay of the data sets and an improved comparison of vector data to the correctly-registered images based on the physical relationships between the vector data and the underlying image features. By incorporating metadata regarding landmarks and other points of interest, users may query target vectors to display attributes of the associated landmarks and create a highly accurate parcel map for validating image information in areas where the data was acquired in sub-optimal conditions.

An image processing apparatus according to an embodiment of the present invention has a first compressing section which compresses each block of an image into first compressed data, a first code converting section which converts the first compressed data into second compressed data, a second code converting section which converts the second compressed data into third compressed data, and a decoding section which decodes the third compressed data. In this case, each block of the second compressed data has a code length equal to or different from that of each block of the first compressed data. Each block of the third compressed data has a code length equal to that of each block of the first compressed data.

The invention belongs to the field of biotechnology and in particular relates to a Cas9 nuclease and application thereof. The Cas9 nuclease (Cas9-R780A) has Cas9 nuclease activity and is suitable for being used in a CRISPR/Cas9 system; theCas9 nuclease is prepared by mutating arginine at the 780 site of the wild-type Cas9 nuclease to alanine. DNA double strands can be cut by using the Cas9 nuclease (Cas9-R780A) so as to generate a protruded cleavage terminal, and then a base complementary to the protruded cleavage terminal can be added in a continuity filling-in manner, so that the precise editing of a specific position of a genomic DNA fragment can be realized.

The invention discloses a semantic high-precision map construction method based on dot-line feature fusion laser, which comprises the following steps: 1) performing semantic segmentation and feature extraction on a visual image acquired by a camera to obtain a visual image containing semantic categories and dot-line features, and then obtaining a foreground and a background of a moving target; 2)projecting the laser three-dimensional point cloud acquired by the laser radar onto a visual image plane, fitting to obtain a depth map, and endowing the laser three-dimensional point cloud with semantic categories and dot line features; 3) performing super-pixel segmentation on the moving target, calculating the distance between super-pixel blocks, constructing a graph model, performing image segmentation, and accurately extracting the boundary of the moving target; and 4) removing the visual feature points and the laser three-dimensional points belonging to the moving target to construct a static high-precision semantic three-dimensional map. The invention further discloses athe positioning method of the semantic high-precision map constructed by adopting the method, and the mapping is more accurate and reliable by accurately removing the moving target.

The invention belongs to the technical field of biology and particularly relates to Cas9 nuclease and application. The Cas9 nuclease (Cas9-K918A) disclosed by the invention has the activity of the Cas9 nuclease, is suitable for a CRISPR/Cas9 system and is obtained by mutating 918th lysine of wild type Cas9 nuclease into alanine. The Cas9 nuclease (Cas9-K918A) is adopted for cutting DNA (Deoxyribonucleic Acid) double strands to generate a predominantly-broken terminal; a basic group complemented with the predominantly-broken terminal can be added in a connection filling-in mode, so that accurate edition of a specific position of a genomic DNA fragment can be realized.

The invention relates to a novel guide RNA (Ribonucleic Acid) expression cassette used in a CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats) system. According to the guide RNA expression cassette, a 5S rRNA gene is adopted as a starter to start expression of guide RNA. The invention further provides a CRISPR/Cas system with the guide RNA expression cassette and a method for editing genomes by using the system. The guide RNA expression cassette, the CRISPR/Cas system and the genome edition method provided by the invention have the advantages of universality, high efficiency, convenience, accuracy and the like.

The embodiment of the invention discloses a particle rendering method and device, an electronic device and a storage medium, which relate to the computer technical field, and can determine the particle state of particles generated by a particle system at any time, so as to carry out accurate editing on a played particle picture. The method comprises the following steps of: when particles of a particle rendering picture are generated, caching particle state information of the particles at a boundary time, wherein the boundary time includes an initial time and an end time; determining a particlestate of the particles at any specified time according to the cached particle state information; updating the particle rendering picture according to the determined particle state to freeze the particle rendering picture to the specified time. The invention can be used in particle rendering.

The invention relates to a method for preparing general type CAR-T cells by using CRISPR/Cas9+AAV, and belongs to the field of immunocytotherapy. The method utilizes CRISPR/Cas9+AAV to knock out an immunological checkpoint PD-1, a human leukocyte antigen (HLA) and TCR; multi-gene knockout is employed to prepare the general type CAR-T cells; and a CRISPR/Cas9 gene editing technology is used to perform precise gene knockout, and is used in combination with adeno-associated virus (AAV) to replace lentiviruses for precise gene knock-in and to avoid the potential risk of random integration of the lentiviruses. The method of the invention can realize multi-target gene knock-in and knock-out, construct the general type CAR-T cells for allogeneic treatment, and solve the problems existing in traditional CAR-T cell therapy such as risk of random insertion mutation of lentiviruses, high production cost and long preparation period for autologous CAR-T design and culture, and insufficient cell number and expansion ability.

The invention discloses a natural language programming method and device based on a strong static script language. The natural language programming method comprises the steps that a front-end compilerobtains a first script and meta-information from a database, complies the first script into a loaded editor renderer, and verifies the content in the editor renderer according to the meta-information, wherein the meta-information at least comprises type information; by editing the verified editor renderer, a second script is generated, and the second script is stored into the database; and a back-end interpreter acquires the second script and the meta-information from the database, compiles the second script into a runtime object, and verifies the runtime object according to the meta-information. By adopting the natural language editor, the readability of functions or scripts is improved, and non-professional programmers are helped to quickly and correctly compile business logic programs,and the function of a function is introduced, and the reusability of rule logic is improved, and the non-programmers are endowed with flexible and powerful program editing capacity, and the natural language programming method is easier to understand and safer.

The invention discloses a method and a system for processing an audio frequency and a video frequency, equipment and a storage medium. The method comprises the following steps: generating the positioning information tag, the environmental meteorological information tag and the time information tag of a position where a recorded audio frequency and video frequency is positioned; matching a historical audio frequency and video frequency which is identical to the information tags from a database; on the basis of the recorded audio frequency and video frequency and the historical audio frequency and video frequency, generating a target audio frequency and video frequency. The method and a system for processing the audio frequency and the video frequency, the equipment and the storage medium have the beneficial effects that the positioning information tag, the environmental meteorological information tag and the time information tag of the position are added to the recorded audio frequencyand video frequency, the historical audio frequency and video frequency which has the same information tag is taken as a reference for recording the audio frequency and the video frequency when the audio frequency and the video frequency are processed, so that the audio frequency and the video frequency can be more accurately edited and processed, the operation difficulty and the operation time ofa user are lowered, and authenticity for processing post-treatment audio frequency and video frequency can be improved.

Genetically engineered hematopoietic cells such as hematopoietic stem cells having one or more genetically edited genes of lineage-specific cell-surface proteins and therapeutic uses thereof, either alone or in combination with immune therapy that targets the lineage-specific cell-surface proteins.

The present invention provides a preparation method for an anti-porcine reproductive and respiratory syndrome cloned pig, comprising: transferring CRISPR/Cas9 targeting vectors and CD163 gene homologous recombination modification vectors into fibroblasts of a pig to obtain positive clone cells, a seventh exon of porcine endogenous CD163 gene being replaced with a eleventh exon of human CD163-L1 gene, so that the positive clone cells are incapable of mediating invasions of PRRSV; taking the positive cell as nuclear transfer donor cells and oocytes as nuclear transfer recipient cells to obtain a cloned embryo by adopting a somatic cell nuclear transfer technology; and impregenating a pig by transferring the cloned embryo into the uterus of the pig, to obtain a cloned pig.

A firmware loading device applied to a data processing device having a host circuit board and a case for receiving the host circuit board is proposed. A plurality of firmwares corresponding to the type of the case are stored in a database. A first electrically connecting unit and a corresponding second electrically connecting unit are respectively provided on the host circuit board and the case. A detecting unit serves to detect an electrical connection relationship between the first electrically connecting unit and the second electrically connecting unit after the host circuit board is assembled in the case. A control unit serves for fetch a firmware appropriate to the case from the database and load the firmware into the host circuit board according to the electrical connection relationship between the first and second electrically connecting units detected by the detecting unit.

The invention provides a Chinese input method system special for medical science. The Chinese input method system consists of a control module as well as a word management module, a rule management module and a grammar management module which are correlated with the control module, wherein the control module is externally connected to an input information extraction and management module; the word management module is used for storing medical thesaurus contents; the rule management module is used for storing medical rule requirements; the grammar management module is used for storing medical grammar information; after the control module receives user information, matched special words are actively searched in a word database unit and conflict check and promotion are performed for users to select and edit. According to the Chinese input method system, the probabilities of single-word input and single-word search of the users are reduced, the searching difficulty of the users is reduced and the input speed and efficiency of the users are increased. The Chinese input method system is suitable for hospitals and medical service industry and various medical fields.

The invention discloses a cytosine deaminase formed by a C-terminal domain of cytosine deaminase APOBEC3B, and a cytosine editor fusion protein containing the cytosine deaminase, a CRISPR/Cas system related protein with DNA (Deoxyribose Nucleic Acid) single-chain cleavage activity, and an optional uracil glycosylase inhibitor.

The invention provides a payload test method based on a configuration test sequence, which comprises the following steps: step 1) reading payload design instruction information, managing the design instruction information in a layered instruction tree form, and providing quick retrieval and positioning of the instruction information; 2) selecting an instruction from the layered instruction tree toadd a test sequence instruction, and performing graphical editing on the added instruction to generate a payload test sequence; 3) detecting the consistency of the effective load test sequence and the load design instruction information; And 4) after the consistency detection is passed, executing the payload test sequence, and generating an instruction sending record and an execution log list inthe execution process of the test sequence to form a test coverage analysis report and a test sequence execution report. Test sequence errors caused by manual careless omission can be effectively avoided, the test efficiency is improved, an analysis report is provided, and an objective basis is provided for a payload user to grasp the technical state of the equipment.

The invention relates to the technical field of targeted editing of a buffalo 18S rDNA gene, in particular to a method for targeted editing of the buffalo 18S rDNA gene by virtue of an adenovirus system. According to the method provided by the invention, an autonomously constructed 18S gene editing vector is used for editing the buffalo 18S rDNA gene; a constructed adenovirus vector consists of Cas9 protein and sgRNA; and then, the vector is subjected to adenovirus packaging. The buffalo 18S rDNA gene is edited on the basis of the accurate and efficient performances of a Cas9 editing gene, a strong bearing capacity of adenovirus on exogenous genes as well as a strong infection capacity of the adenovirus on primary cells, so that a bottleneck that the buffalo primary cells are difficult for transfection; and the packaged adenovirus can efficiently infect buffalo primary mammary epithelial cells and can efficiently edit the buffalo 18S rDNA gene.