Antibody detection kit of porcine epidemic diarrhea virus IgG

A technology for porcine epidemic diarrhea and antibody detection, applied in the biological field, can solve the problems of not reaching the market, increasing the burden on farmers, etc., and achieving the effects of easy differentiation, stable material source, and simple operation process

Inactive Publication Date: 2015-12-02
NANYANG NORMAL UNIV
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Problems solved by technology

However, the current domestic technical force has not reached the corresponding international level, and there has been no Chinese own porcine epidemic diarrhea virus ELISA antibody detection kit on the market for a long time
However, the imported porcine epidemic diarrhea virus ELISA antibody detection kit is expensive, and the cost of clinical application is high, which greatly incr

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  • Antibody detection kit of porcine epidemic diarrhea virus IgG
  • Antibody detection kit of porcine epidemic diarrhea virus IgG

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Embodiment Construction

[0026] A porcine epidemic diarrhea virus IgG antibody detection kit, the kit is equipped with five 96-well immunoassay reaction plates coated with porcine epidemic diarrhea virus antigen, one bottle of 120mL 10-fold concentrated washing solution, and one bottle of 120mL sample diluent , 1 tube of 50 μL positive serum, 1 tube of 50 μL negative serum, 1 tube of 20 μL horseradish peroxide-labeled SPA enzyme-labeled antibody, 1 bottle of 60 mL substrate chromogenic solution A, 1.5 mL each tube of substrate chromogenic solution B Liquid 2 tubes.

[0027] The preparation method of the porcine epidemic diarrhea virus antigen 96-hole immunoassay reaction plate coated is as follows:

[0028] (1) Pour off the culture medium of the monolayer monkey kidney cells in the culture flask, and then add PBS washing solution to the culture flask, 2 The PBS washing solution corresponding to the monolayer of monkey kidney cells is 0.027mL, then shake the culture bottle to make the washing solution...

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Abstract

The invention belongs to the field of biotechnology, and concretely relates to an antibody detection kit of porcine epidemic diarrhea virus IgG. The kit prepared by the invention has a simple operation flow for clinic sample detection, the detection is completed during 3.5 hours, only a common inverted microscope is needed for determination of result, special apparatuses are not needed, specific dyeing is visible to naked eye, non-specific dyeing is easy to be distinguished, so the kit prepared by the invention is convenient for laboratory and clinic on-site detection. A secondary antibody used in the kit prepared by the invention is an enzyme-labeling SPA which can be combined with the mammal IgG with good specificity; the kit provided by the invention can be used for detecting pigs which are infected by the porcine epidemic diarrhea virus or specific IgG antibody in the vaccine inoculated pig, simultaneously is used for evaluating level of the porcine epidemic diarrhea virus antibody IgG during the porcine epidemic diarrhea virus immunization research using mice or rabbit as experiment animal.

Description

Technical field [0001] The invention is a biotechnology field, which involves a pork flow diarrhea virus IgG antibody detection kit. Background technique [0002] Pig fluid diarrhea virus disease is one of the major epidemic infectious diseases that endanger the pig industry in my country. It is a highly exposed to infectious disease with a high degree of contact and high mortality.At present, the new poison strains in my country are infected with new species and ages. The infection rate of newborn piglets in one week is as high as 100 %, and the mortality rate is 50 % to 100 %.In the past, old poison strains only caused local popularity. Since 2011, my country has begun to detect new mutant strains of the virus, causing huge losses.It is reported that between the United States from March 2013 and May 2014, the newly popular virus strain infection caused 5 million pigs to die, and the loss was shocking.The disease has been popular in my country for more than 3 years, and the econ...

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Application Information

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IPC IPC(8): G01N33/569
Inventor 唐青海阚云超姚伦广唐存多卜宾焦铸锦史鸿飞冷超粮刘宗才刘飞
Owner NANYANG NORMAL UNIV
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