Antibody detection kit of porcine epidemic diarrhea virus IgG

Abstract

The invention belongs to the field of biotechnology, and concretely relates to an antibody detection kit of porcine epidemic diarrhea virus IgG. The kit prepared by the invention has a simple operation flow for clinic sample detection, the detection is completed during 3.5 hours, only a common inverted microscope is needed for determination of result, special apparatuses are not needed, specific dyeing is visible to naked eye, non-specific dyeing is easy to be distinguished, so the kit prepared by the invention is convenient for laboratory and clinic on-site detection. A secondary antibody used in the kit prepared by the invention is an enzyme-labeling SPA which can be combined with the mammal IgG with good specificity; the kit provided by the invention can be used for detecting pigs which are infected by the porcine epidemic diarrhea virus or specific IgG antibody in the vaccine inoculated pig, simultaneously is used for evaluating level of the porcine epidemic diarrhea virus antibody IgG during the porcine epidemic diarrhea virus immunization research using mice or rabbit as experiment animal.

Description

Technical field [0001] The invention is a biotechnology field, which involves a pork flow diarrhea virus IgG antibody detection kit. Background technique [0002] Pig fluid diarrhea virus disease is one of the major epidemic infectious diseases that endanger the pig industry in my country. It is a highly exposed to infectious disease with a high degree of contact and high mortality.At present, the new poison strains in my country are infected with new species and ages. The infection rate of newborn piglets in one week is as high as 100 %, and the mortality rate is 50 % to 100 %.In the past, old poison strains only caused local popularity. Since 2011, my country has begun to detect new mutant strains of the virus, causing huge losses.It is reported that between the United States from March 2013 and May 2014, the newly popular virus strain infection caused 5 million pigs to die, and the loss was shocking.The disease has been popular in my country for more than 3 years, and the econ...

AI Technical Summary

Problems solved by technology

However, the current domestic technical force has not reached the corresponding international level, and there has been no Chinese own porcine epidemic diarrhea virus ELISA antibody detection kit on the market for a long time

However, the imported porcine epidemic diarrhea virus ELISA antibody detection kit is expensive, and the cost of clinical application is high, which greatly increases the burden on farmers

[0004] In view of the above, it is necessary to develop a porcine epidemic diarrhea virus antibody detection kit suitable for industrial batch production, with controllable quality, stable performance, high throughput, low cost, specificity and sensitivity. , the cost of the kit should not be too high, otherwise, it will be difficult to popularize and apply

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