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A detection kit for canine parvovirus IgG antibody

A technology for detection of canine parvovirus and antibody, which is applied in the biological field, can solve the problems that the cost of kits should not be too high, the market cannot be achieved, etc., and achieve the effect of low cost of materials, simple preparation process, and stable source of materials

Active Publication Date: 2016-03-30
湖南普菲特动物保健有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current domestic technical strength has not reached the corresponding international level, and there has been no Chinese own canine parvovirus ELISA antibody detection kit on the market for a long time
However, the imported canine parvovirus ELISA antibody detection kit is expensive, and the cost of clinical application is high, so it is difficult to popularize and use it in China.
[0004] In view of the above, it is necessary to develop a canine parvovirus antibody detection kit suitable for industrial mass production, with controllable quality, stable performance, high throughput, specificity and sensitivity. More importantly, the kit The cost should not be too high, otherwise, it will be difficult to popularize and apply

Method used

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  • A detection kit for canine parvovirus IgG antibody
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preparation example Construction

[0025] The preparation method of the 96-hole immunoassay reaction plate coated with canine parvovirus antigen comprises the following steps:

[0026] (1) Pour off the culture medium of the monolayer cat kidney cells in the culture flask, and then add PBS washing solution into the culture flask, 2 The PBS washing solution corresponding to the single-layer cat kidney cells is 0.027mL, then shake the culture bottle to make the washing solution cover the surface of the single-layer cat kidney cells to remove the residual medium in the culture bottle, and pour off the PBS washing solution for later use;

[0027] (2), add trypsin-EDTA-Na with a mass concentration of 0.25% to the culture flask treated in step (1) 2 solution, per cm 2 Trypsin-EDTA-Na corresponding to monolayer cat kidney cells 2 The solution is 0.013mL, shake the culture flask until trypsin-EDTA-Na 2 The solution covers a monolayer of cat kidney cells, and after standing at room temperature for 1 min, pour out the ...

Embodiment 1

[0038] A canine parvovirus IgG antibody detection kit, which consists of:

[0039] (1) 5 96-well immunoassay reaction plates coated with canine parvovirus antigen

[0040] (2) 1 bottle of 10× washing solution (120mL)

[0041] (3) 1 bottle of sample diluent, 120mL

[0042] (4) 1 tube of positive serum (50 μL)

[0043] (5) Negative serum 1 tube (50μL)

[0044] (6) Horseradish peroxide-labeled SPA enzyme-labeled antibody 1 tube (20 μL)

[0045] (7) 1 bottle of substrate chromogenic solution A (60mL)

[0046] (8) 2 tubes of substrate chromogenic solution B (1.5 mL per tube)

[0047] (9) 1 copy of kit instructions.

[0048] Concrete preparation method of the present invention comprises:

[0049] 1. Preparation process of 96-well immunoassay reaction plate coated with canine parvovirus antigen:

[0050] (1) Take 75cm of cat kidney cells covered with monolayer 2 The culture medium in the cell culture flask was poured out; the culture method of cat kidney cells: all the cultu...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a detection kit for canine parvovirus IgG antibody. The kit prepared by the present invention has a simple operation process in the detection of clinical samples, and the detection can be completed within 3.5 hours. The result judgment only needs an ordinary inverted microscope, no special instrument is needed, and the specific staining can be seen with the naked eye. Non-specific staining is very easy to distinguish, which is convenient for laboratory and clinical field testing. Compared with the high cost, high-tech preparation process and production conditions required for the preparation of existing commercial canine parvovirus ELISA antibody detection kits, the kit prepared by the present invention not only has low material cost, simple preparation process, but also has high stability. Strong, high sensitivity, and high-throughput characteristics are comparable to commercial canine parvovirus ELISA antibody detection kits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for canine parvovirus IgG antibody. Background technique [0002] Canine parvovirus disease is one of the main epidemic infectious diseases that endanger the dog industry in my country. It is a highly contagious and highly contagious infectious disease of dogs. It has the characteristics of acute onset and high mortality. Dogs of different breeds and ages are susceptible to infection, the infection rate of puppies is as high as 100% at about 16 weeks of age, and the mortality rate reaches 10% to 50%. The development and progress of China's pet market is the result of comprehensive promotion of social and economic development, people's attitudes towards pets, population aging and urbanization. As one of the pets that have the closest contact with humans and spend the longest time with humans, dogs are inseparable from human daily life, and their health stat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/531G01N33/543G01N33/56983
Inventor 唐青海阚云超姚伦广唐存多毛倩倩焦铸锦史鸿飞冷超粮刘宗才刘飞
Owner 湖南普菲特动物保健有限公司
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