A detection kit for canine adenovirus type Ⅱ IgG antibody

A canine adenovirus and antibody detection technology, which is applied in the biological field, can solve the problems of listing, no canine adenovirus ELISA antibody detection kit, and the cost of the kit cannot be too high, and achieves the effect of simple operation process and stable material source.

Active Publication Date: 2017-06-16
湖南普菲特动物保健有限公司
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Problems solved by technology

However, the current domestic technical strength has not reached the corresponding international level, and there has been no Chinese canine adenovirus ELISA antibody detection kit on the market for a long time
[0004] In view of the above, it is necessary to develop a detection kit for canine adenovirus type Ⅱ antibody that is suitable for industrialized mass production, with controllable quality, stable performance, high throughput, low cost, specificity and sensitivity. , the cost of the kit should not be too high, otherwise, it will be difficult to popularize and apply

Method used

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  • A detection kit for canine adenovirus type Ⅱ IgG antibody

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Embodiment Construction

[0023] A canine adenovirus type II IgG antibody detection kit, which is equipped with 5 96-well immunodetection reaction plates coated with canine adenovirus antigen, 1 bottle of 120mL 10 times concentrated washing solution, 1 bottle of 120mL sample diluent, 1 tube of 50 µL positive serum, 1 tube of 50 µL negative serum, 1 tube of 20 µL horseradish peroxide-labeled SPA enzyme-labeled antibody, 1 bottle of 60mL substrate color solution A, 1.5 mL of substrate color development per tube Liquid B liquid 2 tubes.

[0024] The preparation method of the 96-well immunoassay reaction plate coated with canine adenovirus antigen includes the following steps:

[0025] (1). Pour out the medium in the culture flask for culturing monolayer canine kidney cells, and then add PBS washing solution to the culture flask, every cm 2 The PBS washing solution corresponding to the single layer of canine kidney cells is 0.027mL, then shake the culture flask to cover the surface of the single layer of canine...

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Abstract

The invention discloses a canine adenovirus II type IgG antibody detection kit. The kit is internally provided with five 96-pore immune detection reaction plates covered with canine adenovirus antigens, one bottle of 120-mL 10-time concentrated scrubbing solution, one tube of 50-microliter positive blood serum, one tube of 50-microliter negative blood serum, one tube of 20-microliter horseradish peroxidase marked SPA enzyme-labeled antibody, one bottle of 60-mL substrate color developing solution A and two tubes of 1.5-mL substrate color developing solution B. The kit does not need special instruments and specific dyeing can be observed by naked eyes; non-specific dyeing is very easy to distinguish, and the kit is very convenient for laboratory detection and clinical field detection, and can be used for detecting flexibly, rapidly and accurately.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a canine adenovirus type II IgG antibody detection kit. Background technique [0002] Canine adenovirus is the most pathogenic virus in the genus of mammalian adenoviruses. There are 2 serotypes-Serum type I adenovirus can cause canine infectious hepatitis and fox encephalitis, so it is also called fox encephalitis and canine infectious hepatitis; serum type II can cause canine infectious laryngotracheitis And enteritis, animals immunized with a vaccine prepared by serotype Ⅱ adenovirus can resist the attack of serotype Ⅰ and serotype Ⅱ adenovirus, so the current clinical application is mainly based on the commercial vaccine prepared by serotype Ⅱ adenovirus . The disease presents a global epidemic, and it is widespread in wild foxes, bears, coyotes and raccoons. The disease can occur all year round. Dogs and foxes of all genders, ages and breeds are all susceptible to infec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 唐青海阚云超姚伦广唐存多刘宗才焦铸锦李丹丹
Owner 湖南普菲特动物保健有限公司
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