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Mongolian veterinary drug for resisting escherichia coli diarrhea and preparation method thereof

A technology of Escherichia coli and veterinary medicine, which is applied in the direction of antibacterial drugs, pharmaceutical formulas, and resistance to vector-borne diseases. Effects on survival and growth rate

Active Publication Date: 2021-10-29
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under the action of various pathogenic factors, the three roots of the body appear excessive or partial decline, which destroys the body's homeostasis and produces diseases.

Method used

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  • Mongolian veterinary drug for resisting escherichia coli diarrhea and preparation method thereof
  • Mongolian veterinary drug for resisting escherichia coli diarrhea and preparation method thereof
  • Mongolian veterinary drug for resisting escherichia coli diarrhea and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Optimization and screening experiment of Mongolian veterinary drug BBS traditional compound recipe

[0050] 1.1 Experimental consumables and equipment

[0051] 1.1.1 Main reagents

[0052] Ordinary nutrient agar, eosin methylene blue nutrient agar, nutrient broth, ciprofloxacin (batch number: 11220023), purchased from Guangzhou Baiyunshan Pharmaceutical Co., Ltd.; Yunxiang, rhubarb, Gansong, Qumai, etc. were all purchased from Inner Mongolia Tianli Pharmaceutical Co., Ltd.

[0053] 1.1.2 Strains

[0054] Pathogenic Escherichia coli (E.coli O 1) was presented by Professor Ao Ri Gele from the Cattle Production Laboratory of the School of Animal Science, Inner Mongolia Agricultural University. The strain was isolated, identified and preserved by Dr. Yang Siqin in the experimental team of Professor Ao Ri Gele.

[0055] 1.1.3 Experimental animals

[0056] SPF-grade Kunming mice, half male and half female, weighing 23±2g, were purchased from the Experimental A...

Embodiment 2

[0127] Example 2: Clinical cure experiment of Mongolian veterinary drug BBS on calves with Escherichia coli diarrhea

[0128] 2.1 Experimental materials

[0129] 2.1.1 Test animals

[0130] 48 Holstein calves with similar body weight (40±2kg) were selected. They were randomly divided into 6 groups with 8 heads in each group.

[0131] 2.1.2 Experimental drug

[0132] The 7 flavors of Mongolian medicine were pulverized with a pulverizer, passed through a 200-mesh sieve, and formulated according to the developed dosage, all of which were purchased from Inner Mongolia Tianli Pharmaceutical Co., Ltd. The ciprofloxacin group (production batch number: 20180316) was purchased from Chengdu Xinheng Pharmaceutical Co., Ltd.

[0133] 2.2 Test method

[0134] 2.2.1 Experimental design

[0135] Trials were conducted at the Knight Ranch in Dalat Banner, Ordos, Inner Mongolia, with permission from the company's owners. All experimental protocols have been approved by the Animal Care Co...

Embodiment 3

[0162] Example 3 Effect of Mongolian veterinary drug BBS compound on intestinal mucosal biological barrier in mice with Escherichia coli diarrhea

[0163] 3.1 Experimental materials

[0164] 3.1.1 Experimental bacteria

[0165] Pathogenic Escherichia coli (E.coli O 1 ) was presented by Professor Ao Rigele from the Cattle Production Laboratory of the School of Animal Science, Inner Mongolia Agricultural University, and was isolated, identified and preserved by Dr. Yang Siqin.

[0166] 3.1.2 Experimental animals

[0167] SPF-grade Kunming mice, half male and half female, weighing 23±2g, were purchased from the Experimental Animal Center of Inner Mongolia University, license number: SCXK (Mongolia 2016-0001). In a separate dry ventilated cage, 10 per cage, constant temperature (21±2°C), constant humidity (53±5%), free access to food and water, light / dark cycle of 12h. Adapt to feeding for 3 days and carry out experiments. Experiments were performed in accordance with protoco...

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Abstract

The invention discloses a Mongolian veterinary drug for resisting Escherichia coli diarrhea and a preparation method thereof, the Mongolian veterinary drug comprises kusnezoff monkshood leaf, myrobalan, oxytropis myriophylla, rubia cordifolia, black myrobalan, rhizoma nardostachyos and rheum officinale according to a mass ratio of 2: 1: 1: 1: 1: 2: 2. According to the invention, the anti-inflammatory, immune and intestinal barrier effects of diarrhea model animals and target animals are adjusted through improving the structure of animal intestinal flora., the antibacterial, anti-inflammatory and intestinal barrier functions of the animals are improved, the morbidity of escherichia coli diarrhea is reduced, the survival rate of young animals is further increased, and economic benefits are improved.

Description

technical field [0001] The invention belongs to the technical field of Mongolian veterinary medicine preparation, and in particular relates to a Mongolian veterinary medicine for resisting Escherichia coli diarrhea and a preparation method thereof. Background technique [0002] Escherichia coli disease is highly contagious and has a high mortality rate. Diarrhea is a common clinical symptom of the disease and one of the three major causes of death related to infectious diseases, mainly affecting young animals. According to the National Animal Health Surveillance System in the United States, about 57.0% of sick calves died of diarrhea. In China, the morbidity rate of 10-day-old calves reached 30.27%, and the mortality rate was 4.73%. The incidence rate is higher, reaching 18.87% to 21.26%. In the northwestern region including Inner Mongolia, the positive detection rate of E. coli-induced diarrhea is 35.90%, and the detection rate of E. coli-induced diarrhea in Xinjiang calve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/84A61P31/04A61P1/12
CPCA61K36/714A61K36/185A61K36/48A61K36/74A61K36/328A61K36/84A61K36/708A61P31/04A61P1/12A61K2300/00Y02A50/30
Inventor 王纯洁高瑞娟敖日格乐刘佳乐田艳萍高钰思付鹤杭盖巴特尔包福祥徐进张琨任书男吕文亭高欣
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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