A Simple and Quick Method for Cultivating Barnacle Larvae

A simple and convenient technique for barnacle larvae, which is applied in the field of quick and easy cultivation of barnacle larvae, can solve problems that have not been seen, and achieve the effects of increasing development speed, broad application prospects, and shortening the cultivation time

Active Publication Date: 2019-09-10
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there are no reports in this regard in the existing technologies both at home and abroad.

Method used

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  • A Simple and Quick Method for Cultivating Barnacle Larvae
  • A Simple and Quick Method for Cultivating Barnacle Larvae
  • A Simple and Quick Method for Cultivating Barnacle Larvae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The utensils used for the cultivation of barnacle larvae are cleaned, high-temperature sterilized and dried; the seawater needs to be treated by sand filtration, boiling and disinfection, and then cooled to room temperature for later use.

[0029] The mature individuals of the barnacles Linia chinensis were collected from the reefs along the coast of Daya Bay. During the collection process, try to select individuals that are larger and intact in appearance. Adult barnacles were dissected, and brown mature fertilized eggs were collected, put into a 200mL transparent sample bottle filled with 150mL filtered and sterilized seawater, brought back to the laboratory, and allowed to stand overnight at room temperature (about 24-26°C). After a large number of egg masses hatch, collect lively and robust nauplii and culture them according to the following steps:

[0030] (1) Use a 1000mL glass beaker as a culture container, add an appropriate amount of filtered and sterilized sea...

Embodiment 2

[0038] The utensils used for the cultivation of barnacle larvae are cleaned, high-temperature sterilized and dried; the seawater needs to be treated by sand filtration, boiling and disinfection, and then cooled to room temperature for later use.

[0039] The mature individuals of the barnacles Linia chinensis were collected from the reefs along the coast of Daya Bay. During the collection process, try to select individuals that are larger and intact in appearance. Adult barnacles were dissected, and brown mature fertilized eggs were collected, put into a 200mL transparent sample bottle filled with 150mL filtered and sterilized seawater and brought back to the laboratory. After a large number of larvae hatch, collect lively and robust individuals and cultivate them according to the following steps:

[0040] (1) Use a 1000mL glass beaker as a culture container, add an appropriate amount of filtered and sterilized seawater and bait (about 1000mL) into the beaker, transfer lively ...

Embodiment 3

[0049] The utensils used for the cultivation of barnacle larvae are cleaned, high-temperature sterilized and dried; the seawater needs to be treated by sand filtration, boiling and disinfection, and then cooled to room temperature for later use.

[0050] The mature individuals of the barnacles Linia chinensis were collected from the reefs along the coast of Daya Bay. During the collection process, try to select individuals that are larger and intact in appearance. Adult barnacles were dissected, and brown mature fertilized eggs were collected, put into a 200mL transparent sample bottle filled with 150mL filtered and sterilized seawater and brought back to the laboratory. After a large number of larvae hatch, collect lively and robust individuals and cultivate them according to the following steps:

[0051] (1) Use a 1000mL glass beaker as a culture container, add an appropriate amount of filtered and sterilized seawater and bait (about 1000mL) into the beaker, transfer lively ...

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PUM

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Abstract

The invention discloses a simple and quick method for cultivating larvae of Tetraclita squamosa squamosa. After the nauplius larvae are hatched from eggs of Tetraclita squamosa squamosa, Platymonas subcordiformis and Dicrateria zhanjiangensis are added as bait for cultivation. Through the method, the development speed of the nauplius larvae of Tetraclita squamosa squamosa can be improved, the cultivation time can be shortened, and experimental steps can be simplified, so that convenience is provided for research of marine basic ecology and environmental toxicology; therefore, the method has awide application prospect.

Description

[0001] Technical field: [0002] The invention belongs to the field of biotechnology, and in particular relates to a simple and quick method for cultivating barnacle larvae. [0003] Background technique: [0004] Scale barnacle (Tetraclita squamosa squamosa (Bruguière, 1789)) belongs to the phylum Arthropoda Crustacea subphylum Mandropedia Coleopod subclass Citropodia Perithorax order Sessile order Barnacle suborder Lily barnacle superfamily Lily vine The genus Lily barnacles of the Potaceae Lily barnacle subfamily is widely distributed along the coasts of the East my country Sea and the South China Sea, inhabits the intertidal and subtidal zones, and often attaches to rocks, docks or buoys, becoming an important marine fouling biome. member. [0005] The life history of barnacles is composed of two stages: planktonic life and sessile life. Among them, the period from the time when the nauplius escapes from the egg membrane to the period when the Venus larvae explores and selec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 曹文浩严涛丰美萍韩帅帅林明晴
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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