Method for evaluating influence of ionic liquid on lipid metabolism by using zebra fish

A technology of ionic liquid and lipid metabolism, which is applied in the field of environmental toxicology models, can solve the problems that there is no special research on the toxicity of liver lipid metabolism, and achieve the effects of reliable detection results, low cost and easy cultivation

Pending Publication Date: 2020-07-03
JIANGSU UNIV
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Problems solved by technology

As the liver is the center of metabolism, there is no special rese

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  • Method for evaluating influence of ionic liquid on lipid metabolism by using zebra fish
  • Method for evaluating influence of ionic liquid on lipid metabolism by using zebra fish
  • Method for evaluating influence of ionic liquid on lipid metabolism by using zebra fish

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Embodiment 1

[0028] A method for evaluating the effect of ionic liquids on lipid metabolism by using zebrafish, specifically comprising the following steps:

[0029] Four treatment groups (the exposure concentrations of ionic liquids were 50, 100, 200, 400 mg / L respectively) and one blank control group were set. Three parallel groups were set up for each group, and each parallel contained 2L culture water and 10 healthy adult zebrafish. The exposure experiment started after 10 days of normal domestication. During the exposure period, the water temperature was kept between 21-25°C, the animals were fed twice a day, kept in the light for 14 hours and in the dark for 10 hours, and changed the water every 24 hours. After 15 days of exposure, the experimental samples were taken to measure the corresponding indicators.

[0030] Screen the same batch of healthy 9-month-old adult zebrafish and place them in the same breeding environment for normal domestication for 10 days, and then randomly divi...

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Abstract

The invention relates to the field of environmental toxicology models, in particular to a method for evaluating the influence of ionic liquid on lipid metabolism by using zebra fish. According to theinvention, zebra fish is used as an experimental object; and after acute exposure of the zebra fish in an ionic liquid with a certain concentration, related hormones influencing liver metabolism, physiological and biochemical indexes related to liver metabolism and expression of liver lipid metabolism related genes are determined, and histological slices of the liver are observed. By analyzing thechange of the indexes of the exposed zebra fish, the influence of the ionic liquid on the liver lipid metabolism toxicity is comprehensively and accurately evaluated. Thus, a basis is provided for formulating aquatic ecological environment pollutant standards.

Description

technical field [0001] The invention relates to the field of environmental toxicology models, in particular to a method for evaluating the influence of ionic liquid on lipid metabolism by using zebrafish. Background technique [0002] Ionic liquid (Ionic Liquid) refers to a class of liquid composed of organic cations and inorganic or organic anions. Since Walden discovered and synthesized the first ionic liquid, nitroethylamine, in 1914, the synthesis of ionic liquids has become a research hotspot for researchers, and various ionic liquids have been developed one after another. According to the different organic cations in its composition, it can be simply divided into dialkyl imidazolium ions, alkyl quaternary phosphorus ions, alkyl pyridinium ions and alkyl quaternary ammonium ions. Due to their non-volatile, non-flammable, and strong electrical conductivity, ionic liquids are widely used in the fields of electrochemistry, catalysis, and separation. However, with the dev...

Claims

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Application Information

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IPC IPC(8): G16B40/00G16B25/20C12Q1/6883C12Q1/61
CPCG16B40/00G16B25/20C12Q1/6883C12Q1/61C12Q2600/158C12Q2600/142
Inventor 周磊薛永来崔雯何锦
Owner JIANGSU UNIV
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