Method for storing mature egg mass of balanus reticulatus utinomi

A technology of reticulated barnacles and mature eggs, which is applied in animal husbandry and other fields, can solve the problems of unseen research and reports, restrictions on the scope of popularization and application, high costs of operation, maintenance and construction, etc., and the method is simple and practical, extending Effects of storing backup time, reducing frequency and cost

Inactive Publication Date: 2012-12-12
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are no relevant studies and reports at home and abroad on the storage method of mature egg masses of reticulated barnacles
Although the establishment of an indoor seawater circulation and control system can cultivate adult barnacles and induce their reproduction, the operation, maintenance and

Method used

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  • Method for storing mature egg mass of balanus reticulatus utinomi
  • Method for storing mature egg mass of balanus reticulatus utinomi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Go to the offshore aquaculture facility to collect mature gonad individuals of reticulated barnacles and then transport them back to the laboratory. Individuals with intact appearance were selected and cleaned with seawater, and randomly divided into cold storage group (reticulated barnacles were placed in a beaker and placed in a refrigerator at 6°C) and room temperature storage group (reticulated barnacles were placed in a beaker and room temperature environment) and room temperature holding group (reticulated barnacles were placed in an indoor culture pool with sterilized seawater and continuously injected with air). The hatching medium of larvae is all sterilized seawater, which is obtained by filtering natural seawater through sand filtration, boiling and sterilizing, cooling to room temperature, and placing it in a high-temperature sterilized vessel for use.

[0019] The experimental results showed that after 24 hours, all the samples in the room temperature stora...

Embodiment 2

[0021] Go to the offshore aquaculture facility to collect mature gonad individuals of reticulated barnacles and then transport them back to the laboratory. Individuals with intact appearance were selected and cleaned with seawater, and were randomly divided into two groups, one of which was directly stored in cold storage (refrigerated group I), and the other group was dissected to extract mature eggs and stored in a refrigerator (refrigerated group II). The refrigeration time is 24 hours and the temperature is 4°C.

[0022] The samples of cold storage group I were dissected and the mature egg masses were incubated in a beaker filled with room temperature sterilized sea water, while the mature egg masses of cold storage group II were also placed in room temperature sterile sea water for incubation. Observe the hatching status of nauplii and collect them. The nauplii were cultured in a dark environment at 29.5-30.5°C for 5 days, and the development of the larvae was observed ...

Embodiment 3

[0028] Go to the offshore aquaculture facility to collect mature gonad individuals of reticulated barnacles and quickly transport them back to the laboratory. Individuals with intact appearance were selected and cleaned with seawater, and were randomly divided into two groups. One group was immediately dissected to collect mature egg masses and hatched and cultured larvae; the other group was stored in a refrigerator at 6°C for 24 hours. hours, and then collect mature egg masses for larval hatching and cultivation. See Example 2 for details of the hatching and culturing methods of the larvae. After 5 days, statistical analysis was made on the survival and growth of the larvae.

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Abstract

The invention discloses a method for storing mature egg mass of balanus reticulatus utinomi. The method comprises collecting mature-gonad individuals of the balanus reticulatus utinomi, selecting intact individuals, using sea water to clean the individuals, then placing the individuals in the environment at 4-6 DEG C, and performing refrigeration storage. The method for storing the mature egg mass of the balanus reticulatus utinomi is simple, convenient and practical, the storing standby time of the mature egg mass of the balanus reticulatus utinomi is effectively prolonged on the premise that hatched nauplius can grow and develop normally, the storing time of the mature egg mass of the balanus reticulatus utinomi can be over 24h, the problems that the mature egg mass of the balanus reticulatus utinomi is difficult to store and reserve and fresh samples need collecting prior to larva cultivation each time are solved, the gap is the technical field is filled, times and costs of field sampling operation are reduced, sample utilization rate is improved, development of larva cultivation work is facilitated, a foundation is laid for development of large-scale balanus larva cultivation work, and the method has wide application prospect in the fields of antifouling agent screening, coating testing, adhesion mechanism study and environmental toxicology research and the like.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for storing mature eggs of reticulated barnacles. Background technique: [0002] Barnacles belong to the sessile cirripedes of the order Perithorax, which live in the intertidal zone and subtidal zone. They often attach to rocks, various ships and artificial facilities in groups. Occupying an important position in the environment, it is also an extremely important group in the fouling biome. [0003] Reticulated barnacles (Balanus reticulatus Utinomi) are widely distributed in tropical and subtropical seas. They are the dominant species of fouling organisms in the coastal areas of South China. They have the characteristics of convenient larvae cultivation and short development cycle, so they are not only the most concerned about marine fouling biological control. At the same time, it is also one of the ideal objects for studying the mechanism of marine organism...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 严涛曹文浩胡煜峰黎祖福李静王春忠
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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