Cell cryopreservation and resuscitation method and preparation system thereof

A preparation system and cryopreservation technology, applied in biochemical equipment and methods, preservation of human or animal bodies, bioreactors/fermenters for specific purposes, etc., can solve problems such as cell spillage and reduction

Inactive Publication Date: 2021-05-11
宋铁英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cryopreservation method of this method reduces the requirements for cryopreservation conditions. Under the condition of no liquid nitrogen, normal cell cryopreservation and recovery can still be carried out, and the storage time can

Method used

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  • Cell cryopreservation and resuscitation method and preparation system thereof
  • Cell cryopreservation and resuscitation method and preparation system thereof
  • Cell cryopreservation and resuscitation method and preparation system thereof

Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0028] The following describes this embodiment in conjunction with the figures. The present invention relates to the technical field of cell cryopreservation, more specifically a cell cryopreservation and recovery preparation system, including a culture solution preparation mechanism 1, a cryopreservation and recovery mechanism 2, a protection erection mechanism 3 and a linkage The closing mechanism 4, the linkage closing mechanism 4 is connected to the cryopreservation and resuscitation mechanism 2, connected to the cell culture mechanism 1 and connected to the cryopreservation and resuscitation mechanism 2, and the protective erection mechanism 3 is connected to the cryopreservation and resuscitation mechanism 2 , the linkage closure mechanism 4 is connected to the protection erection mechanism 3 .

[0029] Use the culture solution preparation mechanism 1 to prepare the culture solution required for cell survival, and use the protective erection mechanism 3 to clamp and prote...

specific Embodiment approach 2

[0031] The present embodiment will be described below in conjunction with the drawings. This embodiment will further illustrate Embodiment 1. The culture solution preparation mechanism 1 includes a rotating suspension ring 1-1, a mixing crucible 1-2, a valve 1-3, a mixing motor 1-4 and The mixing shaft 1-5, the mixing shaft 1-5 is fixedly connected to the output shaft of the mixing motor 1-4, the mixing motor 1-4 is fixedly connected to the mixing crucible 1-2, and the outer wall of the mixing crucible 1-2 is connected in rotation On the suspension ring 1-1, the bottom end of the mixing crucible 1-2 is fixedly connected with a valve 1-3.

[0032] Add the nutrient solution required by various cells into the mixing crucible 1-2, and then drive the mixing shaft 1-5 to stir in the mixing crucible 1-2 through the mixing motor 1-4, so that the mixing shaft 1-5 will mix the various cells. The nutrient solution is stirred and mixed to a uniform state to obtain the culture solution nee...

specific Embodiment approach 3

[0034]The present embodiment will be described below in conjunction with the drawings. This embodiment will further illustrate the second embodiment. The cryopreservation and resuscitation mechanism 2 includes a placement base 2-1, an entry and exit slideway 2-2, a freezer 2-3, and a lift seal. Door 2-4, linkage lever 2-5, refrigerator 2-6 and limit slideway 2-7, limit slideway 2-7 is fixedly connected in the right end of freezer 2-3 inside, two refrigerators 2- 6 are respectively fixedly connected to the front and rear sides of the freezer 2-3, the inside of the freezer 2-3 is fixedly connected to the right end of the base 2-1, and the two entry and exit slideways 2-2 are respectively fixedly connected to the base 2-1 The front and rear sides of the front and rear, the lifting airtight door 2-4 is slidably connected to the left side of the freezer 2-3, and the middle part of the linkage lever 2-5 is rotated and connected to the top of the freezer 2-3, and the top of the lifter...

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Abstract

The invention relates to the technical field of cell cryopreservation, and particularly relates to a cell cryopreservation and resuscitation method and a preparation system thereof. Cells cannot spill out during external vibration or impact. The preparation system comprises a culture solution preparation mechanism, a cryopreservation and resuscitation mechanism, a protective erection mechanism and a linkage sealing mechanism. The method comprises the following steps of: step 1, adding various nutrients into a mixing crucible, and mixing the various nutrients to a uniform state by using a mixing shaft to obtain a culture solution required for cell survival; step 2, then placing a culture dish on a placing ring, adding the culture solution and the cells into the culture dish, and then pressing a cover of the culture dish on the culture dish by using a pressing seat; step 3, driving a movable bottom plate to enter a freezing box by an inlet and outlet air cylinder, and then refrigerating by using two refrigerators to reduce the temperature in the freezing box, so that the cells are cryopreserved; and step 4, driving the movable bottom plate to move out of the freezing box by the inlet and outlet air cylinder, so that the culture dish is at room temperature, and the cells are resuscitated.

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a method for cell cryopreservation and recovery and a preparation system thereof. Background technique [0002] The invention with the publication number CN109601529A discloses a simple cell freezing and recovery method, wherein the freezing of cells includes the following steps: preparing a cell suspension and centrifuging it, resuspending the cell pellet with a cell freezing solution, and then transferring all of it to the cell In the cryopreservation tube, seal it with parafilm, and then transfer it to an ultra-low temperature refrigerator for storage after pre-cooling and freezing in turn; the method of cell recovery includes the following steps: take out the cell cryopreservation tube from the 80°C ultra-low temperature refrigerator, and place it in a water bath at 37-40°C. Shake in the pot, and immediately transfer the cell suspension to a culture bottle fille...

Claims

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Application Information

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IPC IPC(8): C12M1/22C12M1/02C12M1/00A01N1/02
CPCA01N1/0242C12M23/10C12M23/38C12M23/48C12M99/00
Inventor 宋铁英
Owner 宋铁英
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