Method using tissue culture seedlings of red-pulp apples to produce anthocyanin

A technology for red-fleshed apples and tissue culture seedlings is applied in the fields of biology and plant biology, which can solve the problems of unsatisfactory factory production, small receiving area, and low anthocyanin content, and achieves the advantages of avoiding bacterial contamination and preventing browning. Effect

Inactive Publication Date: 2016-11-16
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to utilize a new method to extract anthocyanins, so as to solve the problem that the current red-fleshed apple varieties and cultiva

Method used

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  • Method using tissue culture seedlings of red-pulp apples to produce anthocyanin
  • Method using tissue culture seedlings of red-pulp apples to produce anthocyanin

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Embodiment

[0023] A method for producing anthocyanins from tissue cultured seedlings of red flesh apples, which is characterized in that it comprises the following steps:

[0024] (1) Obtain sterile materials:

[0025] Select the fresh red stem tip of the red flesh apple (2010-15) obtained by crossing Xinjiang red flesh apple with Fuji apple as the explant, soak it in 1% Tween (or washing powder) water for 15 minutes, and then After rinsing with tap water for 20 minutes, soak and disinfect with 75% alcohol for 30 seconds on an ultra-clean workbench, rinse once with sterile water, and then immerse and disinfect with 0.1% mercuric chloride solution for 6-8 minutes, shaking continuously during this period to make explants All sides of the body are in contact with the disinfectant, and finally washed with sterile water 5 times;

[0026] (2) Obtained tissue culture seedlings of red flesh apple by primary culture:

[0027] The explants were inoculated on the induction medium, the induction medium was...

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Abstract

The invention discloses a method using the tissue culture seedlings of red-pulp apples to produce anthocyanin. The method includes the steps of firstly, acquiring sterile materials; secondly, performing primary culture to obtain the tissue culture seedlings of the red-pulp apples; thirdly, performing subculture to obtain a large amount of tissue culture seedlings of the red-pulp apples; fourthly, culturing strong seedlings; fifthly, extracting the anthocyanin and preparing crude products. Experiments prove that the anthocyanin content of the tissue culture seedlings, cultured by the method, of the red-pulp apples can reach up to 359.86U.g<-1>FW, is 7.8 times of that of conventional sucrose media under room temperature and is respectively 1.8 times, 2.8 times, 2.9 times and 3.6 times of those of the pericarp 12 weeks after anthesis, new leaves, pulp 12 weeks after anthesis and flowers of the fruiting 5-year-old red-pulp apple trees. The method has the advantages that the method is not limited by time and raw material shortage, the industrial production of the anthocyanin is achieved by using the tissue culture method, and a new approach is provided for the production of the anthocyanin.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to the field of plant biotechnology, and in particular to a method for producing apple anthocyanins using a tissue culture method. technical background [0002] Anthocyanins are natural water-soluble pigments widely found in the flowers and fruits of plants. They belong to flavonoids and are an antioxidant. The beneficial effects of anthocyanins include: softening blood vessels, lowering blood pressure, promoting metabolism and preventing cardiovascular and cerebrovascular diseases, antioxidant activity, preventing cancer, improving visual function, etc. It is a healthy substance recognized as beneficial to health, while red apple It is rich in anthocyanins. However, at present, the red meat apple varieties and cultivation areas promoted in production in my country are relatively small, and due to the limitation of time and lack of raw materials, they cannot meet the requirements of...

Claims

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Application Information

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IPC IPC(8): C07D311/62A01H4/00
CPCA01H4/005C07D311/62
Inventor 张玉刚孙晓红孙欣项亚刘文莲祝军戴洪义
Owner QINGDAO AGRI UNIV
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