Large-scale production process of uterine membrane stem cells

A technology of uterine stem cells and production process, which is applied in the field of large-scale production of uterine stem cells, and can solve the problems of rapid acquisition, expansion and long-term storage of uterine stem cells.

Inactive Publication Date: 2014-06-11
上海坤爱生物科技股份有限公司
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Problems solved by technology

[0012] The present invention aims at the problems of rapid acquisition, expansion and long-term storage of uterine stem cells as seed cells, and aims to f

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  • Large-scale production process of uterine membrane stem cells

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Embodiment Construction

[0028] The present invention aims at the problems of rapid acquisition, expansion and long-term storage of uterine stem cells used as seed cells, and seeks a method for high-efficiency culture, expansion and long-term storage of uterine stem cells suitable for large-scale production.

[0029] The large-scale production process of uterine stem cells of the present invention will be described in detail below.

[0030] 1. Isolation and extraction of uterine stem cells

[0031]①Prepare 4-10mL of PBS or Hanks solution containing penicillin, streptomycin and amphotericin as the preservation solution; ②Pour the menstrual blood from the second day of menstruation into the preservation solution, and transfer it in a low temperature environment (0-8°C) To the laboratory for processing; ③ Prepare 5-10mL lymphocyte separation medium, slowly add an equal volume of sample to the upper layer of the separation medium, density gradient centrifugation, 300-500g, 20-40 minutes (acceleration and ...

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Abstract

The invention provides a large-scale production process of uterine membrane stem cells. The process comprises the following steps: (1) performing separation extraction and primary culture on the uterine membrane stem cells; (2) performing culture amplification on the uterine membrane stem cells; (3) performing large-scale culture on the uterine membrane stem cells; (4) detecting the uterine membrane stem cells; (5) storing the uterine membrane stem cells in a freezing manner; and (6) storing the uterine membrane stem cells in the freezing manner for a long time and performing recovery detection at regular time. According to the process, the uterine membrane stem cells are obtained from the menstrual blood of a female at a child-bearing period, so that a large quantity of high-quality uterine membrane stem cells rich in functional activity can be obtained in short time by utilizing an in-vitro culture amplification method; and the uterine membrane stem cells are stored in the freezing manner for a long time under the environment of -196 DEG C after being detected to be qualified, thereby providing a large quantity of uterine membrane stem cell resources for clinical and scientific researches in the future. Thus, the great social value and significance is achieved.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and relates to a method for culturing, expanding and long-term storage of stem cells, in particular to a large-scale production process of uterine stem cells. technical background [0002] As we all know, stem cell technology is one of the hottest technologies in life sciences today. Its research content involves almost all biomedical fields. In addition to playing an important role in cell therapy, tissue / organ transplantation and gene therapy, it also plays an important role in developmental biology and new drugs. Development and other aspects also have an important impact. In recent years, major breakthroughs have been made in stem cell research. In 1999 and 2000, the world's most authoritative American magazine "Science" listed stem cells and the Human Genome Project as the top ten scientific breakthroughs of the year, especially in 1999. Stem cell research is listed first. Stem cells ...

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Application Information

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IPC IPC(8): C12N5/074C12N5/0789
Inventor 杜明明陈彦田齐瀚实
Owner 上海坤爱生物科技股份有限公司
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