Large-scale production process of uterine membrane stem cells
A technology of uterine stem cells and production process, which is applied in the field of large-scale production of uterine stem cells, and can solve the problems of rapid acquisition, expansion and long-term storage of uterine stem cells.
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[0028] The present invention aims at the problems of rapid acquisition, expansion and long-term storage of uterine stem cells used as seed cells, and seeks a method for high-efficiency culture, expansion and long-term storage of uterine stem cells suitable for large-scale production.
[0029] The large-scale production process of uterine stem cells of the present invention will be described in detail below.
[0030] 1. Isolation and extraction of uterine stem cells
[0031]①Prepare 4-10mL of PBS or Hanks solution containing penicillin, streptomycin and amphotericin as the preservation solution; ②Pour the menstrual blood from the second day of menstruation into the preservation solution, and transfer it in a low temperature environment (0-8°C) To the laboratory for processing; ③ Prepare 5-10mL lymphocyte separation medium, slowly add an equal volume of sample to the upper layer of the separation medium, density gradient centrifugation, 300-500g, 20-40 minutes (acceleration and ...
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