Scale production process of adipose-derived stem cells

A technology of fat stem cells and production process, which is applied in the field of large-scale production technology of fat stem cells, and can solve the problems of rapid acquisition, expansion and long-term storage of fat stem cells

Inactive Publication Date: 2014-06-11
上海坤爱生物科技股份有限公司
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Problems solved by technology

[0010] Aiming at the problems of rapid acquisition, expansion and long-term storage of adipose stem cells as seed

Method used

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  • Scale production process of adipose-derived stem cells
  • Scale production process of adipose-derived stem cells

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Embodiment Construction

[0027] Aiming at the problems of rapid acquisition, expansion and long-term storage of adipose stem cells used as seed cells, the present invention seeks a method for high-efficiency cultivation, expansion and long-term storage of adipose stem cells suitable for large-scale production.

[0028] The mass production process of adipose stem cells of the present invention will be described in detail below.

[0029] 1. Separation and extraction of fat stem cells:

[0030] Take out the collected human fat, remove the blood vessels and fibers visible to the naked eye in the adipose tissue, wash it repeatedly with balanced salt buffer solution such as PBS or D-Hanks or SPB, remove the residual blood, cut the adipose tissue with scissors, add 0.15- Digest with 0.3% collagenase phosphate buffer at 37°C for 40-60 minutes with uniform shaking, then add an equal volume of DMEM or α-MEM culture solution containing 10-20% FBS to stop the digestion, then transfer to a centrifuge tube, 1200- ...

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Abstract

The invention provides a scale production process of adipose-derived stem cells. The production process comprises the following steps: (1) separating and extracting adipose-derived stem cells; (2) culturing and amplifying the adipose-derived stem cells; (3) detecting the adipose-derived stem cells; (4) culturing the adipose-derived stem cells on a large scale; (5) refrigerating the adipose-derived stem cells for preserving; (6) preserving the adipose-derived stem cells for a long time in a refrigerating manner. An in-vitro scale culturing and amplifying method for adipose-derived stem cells is provided, and a long-term, standard and systemic refrigeration preserving system is established for high-quality and high-activity adipose-derived stem cells, so that sufficient high-quality adipose-derived stem cell resources are provided for clinical treatment and scientific research. The process is low in cost and wide in application prospect.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and specifically relates to a method for culturing and expanding stem cells and cryopreservation, and more specifically relates to a large-scale production process of adipose stem cells. Background technique [0002] In 1999, "Science" rated the research results of human embryonic stem cells as the top ten scientific and technological progress in the world that year. In 2000, Time magazine listed it as the top ten scientific and technological achievements in the world at the end of the 20th century. Genome will simultaneously become the most promising field of development and application in the new century. So far, stem cell research has become one of the most striking hot spots in the fields of biology and medicine in recent years. Stem cells are primitive cells that play the role of "backbone" in the growth and development of biological individuals. They are cell populations with self-ren...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 王康陈彦田齐瀚实
Owner 上海坤爱生物科技股份有限公司
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