Strawberry detoxification tissue culture method under LED condition

A tissue culture and strawberry technology, which is applied in the field of strawberry detoxification tissue culture under LED conditions, can solve the problems of high heat generation, short life of fluorescent tubes, unsatisfactory luminous efficiency, etc., and achieve the effect of improving efficiency and reducing costs

Inactive Publication Date: 2011-06-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, fluorescent tubes were usually used as artificial light sources in plant tissue culture production. However, fluorescent tubes have disadvantages such as short life, high heat generation, and unsatisfactory luminous efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for detoxifying tissue culture of strawberries under LED conditions, carried out as follows:

[0044] 1), the selection of culture conditions, with LED (light-emitting diode) as tissue culture light source, the ratio of red light (630 ± 20nm) and blue light (460 ± 20nm) in LED is 2~5:1~2, and light intensity 1500~2000Lx, the light time is 12~16h / d, and the culture temperature is 25±2℃.

[0045] 2), the preparation of culture medium, including each component of basic culture medium and culture medium of each stage of tissue culture and the contained weight per liter are:

[0046](1) Basic medium: MS basic medium for induction, proliferation, and seedling growth medium; 1 / 2 MS basic medium for rooting medium; among them, sucrose or white sugar 20-40g / L, agar 7-9g / L, pH 5.6~5.8;

[0047] (2) Induction medium: MS+BA0.1~2.0mg / L+NAA0.01~0.5mg / L;

[0048] (3) Proliferation medium: MS+BA0.1~2.0mg / L+NAA0.01~0.5mg / L;

[0049] (4) Strong seedling medium: MS+BA0.1~1.0m...

Embodiment 2

[0070] In this example, LED (light emitting diode) was used as the light source for tissue culture, the ratio of red light and blue light in LED was 3:1, the light intensity was 2000Lx, the light time was 16h / d, and the culture temperature was 25±2°C. Induction medium: MS+BA1.0mg / L+NAA0.2mg / L; Proliferation medium: MS+BA 0.5mg / L+NAA 0.1mg / L; Strong seedling medium: MS+BA 0.1mg / L+NAA0 .05mg / L; rooting medium: 1 / 2MS+IBA 0.5mg / L.

[0071] All the other steps, technique, condition are all the same as embodiment 1.

Embodiment 3

[0073] In this example, LED (light emitting diode) was used as the light source for tissue culture, the ratio of red light and blue light in LED was 2:1, the light intensity was 1500Lx, the light time was 16h / d, and the culture temperature was 25±2°C. Induction medium: MS+BA1.0mg / L+NAA0.2mg / L; Proliferation medium: MS+BA 0.5mg / L+NAA 0.1mg / L; Strong seedling medium: MS+BA 0.1mg / L+NAA0 .05mg / L; rooting medium: 1 / 2MS+IBA 0.5mg / L.

[0074] All the other steps, technique, condition are all the same as embodiment 1.

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Abstract

The invention belongs to the field of plant detoxification tissue culture rapid propagation technology, and mainly relates to a strawberry detoxification tissue culture method under LED condition, which comprises the steps of: selection of culture conditions, preparation of a culture medium, selection and sterilization of an explant, induction culture, propagation culture, strong seedling culture, rooting culture and transplant. The strawberry detoxification tissue culture method has the advantages that an LED is used as a tissue culture light source, the effects of strawberry induction, propagation, strong seedling and rooting culture and the tissue culture seedling quality are not different from those of a fluorescent lamp, or the culturing effect is better than that of the fluorescent lamp; the survival rate of the tissue culture seedlings is higher than 90%, the culture area is enlarged by 40% compared with the area when the fluorescent lamp is used, and the electricity is saved by 30-50%; and the cost is reduced, and the efficiency of strawberry detoxification tissue culture factory production can be improved.

Description

technical field [0001] The invention relates to the technical field of plant detoxification tissue culture and rapid propagation, in particular to a method for detoxification tissue culture of strawberries under LED conditions. Background technique [0002] Strawberry (Fragaria anananssa Ducde) is a perennial evergreen herb of the genus Fragaria in the family Rosaceae. Strawberry has the advantages of easy cultivation, fruit in the same year, high yield, good economic benefits, etc., and it has developed rapidly in various places. However, strawberry is a kind of crop that relies on stolons for asexual reproduction. It is prone to be infected with various viral diseases in production. The infected fruits are getting smaller year by year, deformed, poor quality, shrunken leaves, slow growth, and generally reduce production by 30%~ 80%, and increased year by year. There is currently no effective drug for the prevention and treatment of viral diseases. Cultivating virus-free...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈剑平徐刚陈志汪一婷吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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