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Method for simultaneously extracting taxol and 10-DABIII (10-deacetyl baccatine III) from branches and leaves of Taxus media

A technology of yew and paclitaxel, applied in the direction of organic chemistry, etc., to achieve the effect of increasing the extraction speed, improving the extraction effect, and the method is simple and reliable

Inactive Publication Date: 2011-10-12
CHANGSHAN MANDIYA HONGDOUSHAN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a defect in these existing processes, that is, only one product can be obtained from one extraction, such as obtaining paclitaxel or obtaining 10-DAB III, obtaining paclitaxel by a semi-synthetic method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] After collecting the branches and leaves of Taxus chinensis, place them in an oven at 50°C and dry them, crush them into 100-mesh dry powder, weigh 5g, add 100mL of methanol, ultrasonically treat for 40min, let stand and filter, and filter the residue with 100mL of methanol for 40min, so Repeat 3 times, combine the 3 times of methanol extracts, in a rotary evaporator, 50 ° C water bath, -0.09Mpa vacuum, recover methanol to dryness under reduced pressure.

[0015] Detect the content of paclitaxel and 10-DAB III in the methanol extract by HPLC: recover the methanol under reduced pressure to dryness, add 18mL of methanol and 2mL of water, extract fat-soluble impurities such as pigments with n-hexane several times, and discard the n-hexane solution , the methanol solution was concentrated to dryness, then extracted several times with dichloromethane and water, combined the dichloromethane extracts, placed in a rotary evaporator in a water bath at 40°C, with a vacuum of -0.03...

Embodiment 2

[0017] The test was prepared by solvent extraction of Taxus chinensis branches and leaves: after collecting the branches and leaves of Taxus chinensis, put them in an oven at 50°C for drying, crush them into 100 mesh dry powder, weigh 5g, add 100mL methanol, and place 50°C water bath, 2h, filter, filter residue in 100mL methanol water bath for 2h, repeat this 3 times, combine the 3 methanol extracts, in a rotary evaporator, 50°C water bath, under vacuum of -0.09Mpa, reduce pressure Methanol was recovered to dryness.

[0018] Detect the content of paclitaxel and 10-DAB III in the methanol extract by HPLC: recover the methanol under reduced pressure to dryness, add 18mL of methanol and 2mL of water, extract fat-soluble impurities such as pigments with n-hexane several times, and discard the n-hexane solution , the methanol solution was concentrated to dryness, then extracted several times with dichloromethane and water, combined the dichloromethane extracts, placed in a rotary e...

Embodiment 3

[0020] The test was prepared with the Soxhlet extract of Taxus chinensis branches and leaves: after collecting the branches and leaves of Taxus chinensis, put them in an oven at 50°C for drying, crush them into 100 mesh dry powder, weigh 5g, and place them in a Soxhlet extractor. Add 150mL of methanol, extract at 80°C for 6 hours at a speed of siphoning 3 times per hour. The methanol solution was recovered in a rotary evaporator in a water bath at 50°C and under a vacuum of -0.09Mpa to dryness under reduced pressure.

[0021] Detect the content of paclitaxel and 10-DAB III in the methanol extract by HPLC: recover the methanol under reduced pressure to dryness, add 18mL of methanol and 2mL of water, extract fat-soluble impurities such as pigments with n-hexane several times, and discard the n-hexane solution , the methanol solution was concentrated to dryness, then extracted several times with dichloromethane and water, combined the dichloromethane extracts, placed in a rotary ...

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PUM

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Abstract

The invention relates to a method for simultaneously extracting taxol and 10-DABIII (10-deacetyl baccatine III) from branches and leaves of Taxus media, which separately employs ultrasonic extraction, solvent lixiviation and Soxhlet extraction and comprises the following steps: concentrating an extracted methanol solution to be dry while depressurizing, extracting with n-hexane to remove pigment and other fat-soluble impurities, extracting with dichloromethane to obtain a final extraction liquid of taxol and 10-DABIII, and detecting the contents by HPLC (high-performance liquid chromatography). The method provided by the invention fully takes advantages of the different extraction methods to simultaneously extract taxol and 10-DABIII, improves the utilization rate of resources, and has the characteristics of simplicity, rapid extraction and high efficiency.

Description

technical field [0001] The invention relates to a method for separating and extracting natural organic compounds from branches and leaves of plants, in particular to a method for simultaneously extracting paclitaxel and 10-DAB III from branches and leaves of Taxus mandia. Background technique [0002] Paclitaxel and 10-DAB III (10-deacetylbaccatin III) are natural organic compounds isolated from Taxus chinensis. Paclitaxel is a tetracyclic diterpene compound. It is a natural drug for the treatment of progressive ovarian cancer, breast cancer, leukemia and other cancers. It is also the only natural new anti-tumor drug that promotes tubulin aggregation and irreversible depolymerization. . 10-DAB III is a precursor of paclitaxel. my country has started the research work on the preparation of yew and paclitaxel since 1984. Paclitaxel was first extracted from the Himalayan yew leaf in Tibet, and a new alkaloid three-type mannine with anticancer activity equivalent to paclitaxel...

Claims

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Application Information

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IPC IPC(8): C07D305/14
Inventor 徐志明张新凤史小娟付顺华斯金平
Owner CHANGSHAN MANDIYA HONGDOUSHAN TECH DEV
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