Extraction method of taxol from branches and leaves of artificially cultivated yew

Abstract

The invention relates to an extraction method of taxol from branches and leaves of artificially cultivated yew. A plurality of methods, such as planting of stocky yew, cultivating of tissue and semi-synthesis and total synthesis of taxol, have been developed home and abroad so as to obtain taxol raw material; however, the tissue cultivating method has low yield and high broth price; and the total synthesis has extremely low yield, worse therapeutic effect than natural-source taxol, and a high cost; thereby being difficult for commercial application. The invention uses branches and leaves of artificially cultivated taxus media, Yunnan taxus, southern taxus or northeastern taxus as raw materials; the raw materials are marinated by carbinol and extracted by petroleum oil and propyl acetate in sequence; and normal-pressure normal-phase silica gel column chromatography or reverse-phase high-pressure liquid chromatography is used to obtain high-purity taxol with a purity being higher than 99.6%. The preparation method has simple process, low cost, recyclable solvent and eluant; therefore, the method is applicable to industrial production and protects plant resource.

Description

technical field

[0001] The content of the invention belongs to the technical field of preparation of biochemical products, and relates to a method for extracting paclitaxel from branches and leaves of yew artificially cultivated. Background technique

[0002] Paclitaxel (taxol) is an antineoplastic drug with complex chemical structure and unique mechanism of action extracted from the bark, branches and leaves of Taxus (Taxaceae) plants. The sources of natural paclitaxel are very limited, mainly from yew or yew bark (Wani et al., J. Am. Chem. Soc. 1971, 93, 2325). The growth of yew tree species is extremely slow. Generally, a century-old tree can only peel 25kg of bark. If 1kg of pure taxol is extracted to provide 12,000 cancer patients, it is equivalent to the bark of 30,000 yew trees. . In order to obtain paclitaxel, methods such as planting dwarf yew trees, tissue culture, semi-synthesis and total synthesis of paclitaxel have been carried out at home and abroad to obtain...

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