Riemerella anatipestifer mutant strain with Cas9 gene deletion and applications of riemerella anatipestifer mutant strain

A technology of gene deletion and Ribella, which is applied in the direction of vaccines, bacteria, antibacterial drugs, etc., can solve the problems of no products coming out, and no Ribella anatipestifer has yet been seen, and achieve low production costs, simple cultivation methods, and simple operations fast effect
CN106190903AInactive Publication Date: 2016-12-07HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2016-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a riemerella anatipestifer mutant strain with Cas9 gene deletion and applications of the riemerella anatipestifer mutant strain, belonging to the technical field of preparation of animal genetically engineered vaccines. The strain is preserved in the China Center for Type Culture Collection (CCTCC), and is assigned with the accession number of CCTCC NO:M 2016247. According to the strain, 2070bp of the Cas9 gene of the CRISPR-Cas system is deleted, so that the toxicity of the bacterial strain is obviously reduced, but the good immunogenicity is still reserved. Compared with the traditional inactivated vaccines, the strain with the Cas9 gene deletion has the advantages that the culture method is simple, the production cost is low, the inoculation can be carried out through the manners of nasal inhalation, mist spraying and the like, the operation is simple and rapid, the body can be stimulated to generate effective mucosal immunity, and meanwhile, the systemic immunity of the body also can be stimulated.
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Description

technical field

[0001] The invention belongs to the technical field of animal bacterial genetic engineering, and relates to related fields such as animal molecular biology, microbiology, and immunology. Specifically, it relates to a Cas9 gene deletion mutant strain of R. anatipestifer and an application thereof. Background technique

[0002] Riemerella anatipestifer is an acute, contact, septic infectious disease caused by Riemerella anatipestifer (RA), which mainly occurs in ducks aged 1-8 weeks, especially 2- 3-week-old ducklings are the most susceptible. The disease is characterized by fibrinous pericarditis, perihepatitis, air sacculitis and meningitis, and is one of the most serious bacterial infectious diseases that endanger the duck industry. Diseased ducks have caused huge economic losses to the duck industry due to high mortality, stunted growth, reduced quality, low feed remuneration and increased treatment costs. There are many RA serotypes. At present, 21 sero...

Claims

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