Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application

A porcine pseudorabies virus and respiratory syndrome technology, applied in antiviral agents, virus antigen components, virus/bacteriophage, etc., can solve the problem of unsatisfactory control effect of mutant porcine reproductive and respiratory syndrome virus

Inactive Publication Date: 2009-06-17
HUAZHONG AGRI UNIV
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  • Abstract
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Problems solved by technology

This patent application only involves the prevention and control of non-mutated PRRS virus, and the control effect against mutant PRRS virus is not ideal

Method used

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  • Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application
  • Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application
  • Recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus genetic engineering strain and application

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Embodiment 1

[0041] The present invention will be further described below in conjunction with the accompanying drawings of the description.

[0042] 1. Primer design (for gene cloning and molecular detection)

[0043] Design primers Porf5F and Porf5R according to the ORF5 gene sequence of the isolated porcine reproductive and respiratory syndrome virus (PRRSV) strain WuH3 (preservation number: V200810), the size of the amplified fragment is about 603bp, and EcoR I is designed at both ends , EcoR V enzyme cutting site. At the same time, according to the porcine circovirus type II PCV2 YuA strain on GenBank (Cao Shengbo, Whole Genome Cloning and Sequence Analysis of Porcine Circovirus Type II PCV2 YuA Strain, Acta Virus Sinica, 2002, 18(2): 137-141) ORF2 Gene sequence design primers Porf2F and Porf2R, the size of the amplified fragment is about 580bp (the first 41 amino acids of the ORF2 gene are cut off), and a pair of primers P5 and P6 are also designed to amplify the two expression casse...

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Abstract

The invention belongs to the animal virus genetic engineering technique field, especially to a recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain construction, a vaccine preparation and applications. The recombinant porcine pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus gene engineering strain E001 lacks the pseudorabies virus main virulence gene TK, glycoprotein genes gG, gE and gI, does not express the functional glycoprotein gG and gE / gI of the pseudorabies virus; and simultaneously expresses a GP5m / M protein antigen of the porcine propagate and breath complex virus classical strain and the porcine propagate and breath complex virus internal variant GP5 protein and porcine circovirus ORF2. The strain can stimulate the porcine to produce protective immunity reaction for resisting pseudorabies virus-porcine propagate and breath complex virus-porcine circovirus, effectively prevent the infection of the pseudorabies virus, the porcine propagate and breath complex virus and porcine circovirus. The invention also discloses a preparation and application of a tervalence genetic engineering vaccine.

Description

technical field [0001] The invention belongs to the technical field of animal virus genetic engineering. Specifically relates to the preparation of a recombinant porcine pseudorabies virus-porcine reproductive and respiratory syndrome virus-porcine circovirus genetically engineered strain, the genetically engineered vaccine prepared with the genetically engineered strain and its application. Background technique [0002] Pseudorabies is an acute infectious disease caused by pseudorabies virus (Pseudorabies virus, referred to as PrV) infecting pigs, cattle, sheep, dogs, cats, rabbits, mice, minks, foxes and other domestic and wild animals. Reservoirs and disseminators of viruses (Mettenleiter et al., Comp Immunol Microbiol Infect Dis. 1991, 14: 151-163). Other animals except pigs are in sporadic form, and usually have symptoms such as fever, itching, and encephalomyelitis after the onset, all of which are fatal infections. The disease is an outbreak in pigs, mainly causing ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K39/295A61K39/12A61P31/14A61P31/20C12R1/93
Inventor 肖少波徐凤琴方六荣江云波陈焕春
Owner HUAZHONG AGRI UNIV
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