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Polyethylene imine modified carbon nano-tube, its complexes, production method and uses thereof

A polyethyleneimine, carbon nanotube technology, applied in the field of carbon nanotubes, can solve the problem of not being able to confirm the influence of PEI molecular weight, etc., and achieve the effects of low cytotoxicity and high transfection efficiency

Inactive Publication Date: 2009-05-13
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this literature cannot confirm the effect of PEI molecular weight on transfection efficiency and cytotoxicity

Method used

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  • Polyethylene imine modified carbon nano-tube, its complexes, production method and uses thereof
  • Polyethylene imine modified carbon nano-tube, its complexes, production method and uses thereof
  • Polyethylene imine modified carbon nano-tube, its complexes, production method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] Example 1: Oxidation cutting and polishing of MWCNTs

[0028] Pristine MWCNTs (purchased from Nanometer Port, Shenzhen) were soaked in 6M HCl for seven days, washed sequentially with dilute NaOH solution, deionized water, and dried to obtain pure MWCNTs with demetallized catalysts. Put 100mg of pure MWCNTs into 80ml H at 3:1 2 SO 4 / HNO 3 In the mixed solution, sonicate for 24 hours in a water bath at 40-50°C. Centrifuge (7000rpm, 5min), dilute the precipitate with deionized water, filter through a 0.22μm polytetrafluoroethylene membrane, wash fully with deionized water, put into 4:1 concentrated 50ml H 2 SO 4 / 30%H 2 o 2 The mixed solution was stirred at 70°C for 30 min. to obtain oxidized cut and polished MWCNTs.

example 2

[0029] Example 2: PEI grafted with MWCNTs

[0030] Oxidatively cleaved MWCNTs (20 mg), ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were placed in 50 ml sodium phosphate buffer solution (PBS) , after stirring for 24 h under the protection of nitrogen, polyethyleneimine (Mn=22-600 kDa) was added, and the stirring was continued for 24 h. The reaction mixture was added to a 10-fold excess of absolute ethanol, filtered through a 0.22 μm polytetrafluoroethylene membrane, rinsed with absolute ethanol and deionized water in turn, and the obtained PEI-g-MWCNTs had good dispersibility in deionized water , The suspension is stable for 1 month. While the cut and polished MWCNTs precipitated after 2h.

example 3

[0031] Example 3: Content of polyethyleneimine in PEI-g-MWCNTs

[0032] The weight of supramolecular PEI grafted onto the surface of MWCNTs was obtained by thermal analysis (TGA) in nitrogen atmosphere. The MWCNTs used in the present invention are stable at 600°C, PEI is completely decomposed at 500°C, the weight loss of MWCNTs is about 2.5%, while that of PEI-g-MWCNTs is 10.5%, and the weight of grafted PEI is about 8%. The PEI-grafted MWCNTs were suspended and stable in deionized water for 1 month with good dispersion. However, the MWCNTs of the present invention precipitated after 2 h.

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Abstract

The invention discloses a carbon nanometer tube decorated by polyethyleneimine and a compound of plasmid DNA thereof. The plasmid DNA adopted in the invention comprises P53DNA, green fluorescent protein expression (JPC) and unmethylated oligomerization deoxynucleotide plasmid DNA. The carbon nanometer tube decorated by the polyethyleneimine and the compound of the plasmid DNA have the advantages of high transfection efficiency and less toxicity to cells; in particular, when the concentration is 30 g / ml, the transfection efficiency is larger than 90 percent, and the validity of the cells is larger than 80 percent. The carbon nanometer tube decorated by the polyethyleneimine and the compound of the plasmid DNA have wide application prospect during the process of preparing medicaments for remedying genes outside transfecting osteosarcoma cell bodies and in animal bodies; and the carbon nanometer tube decorated by the polyethylene imine / the plasmid DNA / an adriacin nanometer compound also have wide application during the process of preparing medicaments for restraining the growth of tumor.

Description

technical field [0001] The invention relates to the field of carbon nanotubes, in particular to a polyethyleneimine-modified carbon nanotube (PEI-g-MWCNTs) and a compound thereof, a preparation method and application thereof. Background technique [0002] Using carbon nanotubes as carriers to introduce exogenous DNA into cells has aroused people's research interest. With mixed acid (sulfuric acid: nitric acid = 3:1), the side wall or port of the carbon nanotubes oxidized by nitric acid has carboxyl functional groups, and oligo DNA can be directly adsorbed on the surface of carbon nanotubes and can be introduced into cells. The amino-terminated divinyl-modified carbon nanotubes can concentrate plasmid DNA and oligo DNA through the electrostatic interaction between the cationic functional group and the negative charge on the DNA backbone, and can effectively introduce the plasmid DNA into cells. The gene expression of this functionalized carbon nanotube (f-CNT) / DNA complex wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02
Inventor 于伯章马继飞黄庆
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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