Liquid microbial agent and preparation method and application thereof

A liquid bacterial agent and microorganism technology, applied in the field of microbial liquid bacterial agent and its preparation, can solve the problems of low content of effective bacteria and easy inactivation, and achieve the effects of improving nutrient absorption, promoting rapid proliferation and solving soil acidification.

Inactive Publication Date: 2018-11-13
NANJING SHINEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In order to solve the problem that the effective bacteria content of the existing microbial liquid inoculum is low and easy to inactivate, the present invention provides a kind

Method used

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  • Liquid microbial agent and preparation method and application thereof
  • Liquid microbial agent and preparation method and application thereof
  • Liquid microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The fermentation of embodiment 1 growth-promoting bacteria

[0039] Prepare the growth-promoting bacteria liquid according to the following steps:

[0040] Activate the Bacillus subtilis CCTCC NO:M 2016264, Bacillus jelly-like CCTCC NO:M 2016265, and Bacillus amyloliquefaciens CCTCC NO:M 2016266 stored on a slant at 4°C, and inoculate them in eggplant-shaped culture flask medium (i.e., activation culture Base), cultured at 32°C for 18h. Transfer the activated strains to the fermentation medium, culture at 30°C for 24h, place the fermented liquid in a cold storage at 4°C for 48h at low temperature, and measure by plate coating, the number of viable bacteria in each microbial fermentation liquid can reach 8 ×10 9 CFU / mL or more, the three microbial fermentation liquids are mixed according to the volume ratio of 1:1:1 to obtain the growth-promoting bacteria liquid.

[0041] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation med...

Embodiment 2

[0043] The preparation of embodiment 2 compound microbial liquid inoculum

[0044] Prepare the composite microbial liquid bacterial agent containing polyglutamic acid, biological organic matter, growth-promoting bacterial liquid according to the following method:

[0045] (1) Polyglutamic acid was prepared by fermentation (reference Xu Z, Feng X, Dan Z, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis, NX-2 immobilized in anaerobic plant fibrous-bed bioreactor[J ].Bioresour Technol, 2014,155:8-14), the polyglutamic acid content of the fermented liquid is 15% after concentration;

[0046] (2) The molasses is purchased by the sugar factory;

[0047] (3) Mix the composite growth-promoting bacteria liquid obtained in Example 1 with the polyglutamic acid fermentation concentrate and molasses according to the mass ratio of 1:2.5:6.5 to obtain a composite microbial liquid bacterial agent. After testing, the mass percentage of polyglutamic acid content is 3.5%...

Embodiment 3

[0048] Embodiment 3 Composite microbial liquid inoculum can be kept open for up to 18 months

[0049] The bacterial agent used in the present embodiment is as follows:

[0050] (1) The mixed bacterial agent prepared in Example 1 is diluted to an effective viable count of 7.3 × 10 9 CFU / mL, as bacterial agent 1.

[0051] (2) The mixed bacterial agent prepared in Example 1 is mixed in proportion with the polyglutamic acid fermentation concentrate, so that the polyglutamic acid content is 3.5%, and the effective viable count is 7.3×10 9 CFU / mL, as bacterial agent 2.

[0052] (3) The mixed bacterial agent prepared in Example 1 is mixed uniformly with molasses in proportion, so that the organic matter content is 67%, and the effective viable count is 7.3×10 9 CFU / mL, as bacterial agent 3.

[0053] (4) The mixed bacterial agent prepared in Example 1 is mixed uniformly with polyglutamic acid and molasses in proportion, so that the polyglutamic acid content is 3.5%, the organic ma...

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Abstract

The invention discloses a liquid microbial agent. The liquid microbial agent is prepared from the following components in percentage by mass: 1-10% of a growth-promoting bacterium solution, 0.1-10% ofpolyglutamic acid and 50-70% of a biological organic matter. The invention further discloses a preparation method of the liquid microbial agent. The invention further discloses application of the liquid microbial agent in one or any of improving the crop yield, improving the crop quality and enhancing the crop stress resistance. When the agent is in a packaged state, by providing an high organicmatter environment and a high osmotic pressure environment, growth-promoting bacterium spore germination is inhibited, so that growth-promoting bacteria are in a spore state; when the liquid microbialagent is diluted for application, the organic matter can provide nutrition for spore germination to promote fast proliferation of the growth-promoting bacteria; when the biological organic matter isused in cooperation with the polyglutamic acid, through a synergistic effect, the activity of the growth-promoting bacteria in the liquid microbial agent is maintained, so that the shelf life of the liquid microbial agent is greatly prolonged.

Description

technical field [0001] The invention relates to a microbial inoculum, in particular to a liquid microbial inoculum and a preparation method and application thereof. Background technique [0002] my country is a big agricultural country in the world. With the rapid development of my country's agriculture, the use of chemical fertilizers is also increasing, reaching 1.8 million tons per year. Although this solved the problem of increasing food production and supply, it caused very serious environmental problems. The excessive use of chemical fertilizers has aggravated soil compaction and pollution, leading to the decline and degradation of soil quality, which makes the content of soil organic matter seriously lower than the global average level. Once the soil organic matter content is low, it will greatly reduce the ability to fix heavy metals, increase the harmfulness of soil heavy metals, and endanger the sustainable development of agriculture in our country. [0003] Micr...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C12N1/20C12R1/07C12R1/125C05G3/80
CPCC05C11/00C05F5/00C05G3/00C12N1/20C05G3/80C05G5/20C05F11/08
Inventor 冯小海许宗奇周哲徐虹
Owner NANJING SHINEKING BIOTECH CO LTD
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