The invention provides calcitriol soft capsules with high bioavailability, high stability, convenience in administration, moisture absorption prevention, good sealing property and low price, which improves the bioavailability and the compliance of patients. In order to fulfill the aims, the calcitriol soft capsules and a preparation method thereof are finally obtained through a lot of experimental research; the calcitriol soft capsules comprise a soft capsule material and inclusion liquor, wherein the soft capsule material comprises gelatin, glycerol and water in a weight ratio of 10:3.5:9; and the inclusion liquor comprises calcitriol, co-emulsifier, oil phase and emulsifier in a weight ratio of (1-4): (30,000-35,000): (20,000-25,000): (80,000-100,000). The weight ratio of the soft capsule material to the inclusion liquor is 5:4.