Rapid propagation method for dendrobium hancockii seeds

A technology of seeds and Dendrobium, which is applied in the field of rapid propagation of Dendrobium bamboo-leaf seeds, can solve problems such as no reports of Dendrobium bamboo-leaf dendrobium, and achieve the effect of accelerating the speed of seed propagation

Active Publication Date: 2016-06-08
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Using plant tissue culture technology is an effective way to propagate seedlings in large quantities, but there is no relevant report about Dendrobium

Method used

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  • Rapid propagation method for dendrobium hancockii seeds
  • Rapid propagation method for dendrobium hancockii seeds
  • Rapid propagation method for dendrobium hancockii seeds

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Embodiment 1

[0028] A method for rapidly propagating Dendrobium bamboo-leaf seeds, comprising four stages: a pretreatment stage, a seed germination cultivation stage, a protocorm differentiation cultivation stage and a rooted and strong seedling cultivation stage, and the specific steps are as follows:

[0029] Step (1), the pretreatment stage, the steps are as follows: wipe the surface of the mature fruit pods with 75% alcohol, then treat with 0.1% mercury chloride for 10 minutes, and then treat them with sterile water for 3 times; In alcohol for 30s, take out the water on the pod surface with sterilized filter paper, place it in a sterilized inoculation tray, cut off the pod after cooling, and take out the seeds;

[0030] Step (2), seed germination culture stage: the seeds are evenly inoculated in the seed germination medium to germinate, and the seed germination medium is 1 / 2MS medium; the culture temperature is 25°C, the light intensity is 3000lx, and the light time is 12h / d; after 45 ...

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Abstract

The invention belongs to the technical field of plant tissue culture and discloses a rapid propagation method for dendrobium hancockii seeds. The rapid propagation method comprises a pretreatment stage, a seed germination culture stage, a protocorm differentiation culture stage and a rooting and seedling strengthening culture stage. According to the rapid propagation method for the dendrobium hancockii seeds, at the seed germination stage, a large number of protocorms can appear after 45 d, and the germination rate of the seeds reaches up to 95%; at the protocorm differentiation stage, time for seedlings to be differentiated is controlled within 60 d, optimally 50 d; at the rooting and seedling strengthening culture stage, tissue culture seedlings grow to 3 cm or above after about 45 d, and the number of branches of one root is 2 or above. The period from seed germination to aseptic seedling rooting to acclimatization and transplanting requirement satisfaction is within 150 d, the propagation speed of the dendrobium hancockii seeds is greatly increased, and convenience is brought to rapid production of dendrobium hancockii.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for rapid propagation of dendrobium bamboo-leaved seeds. Background technique [0002] Dendrobium bamboo-leaf, also known as Dendrobium fine-leaf Dendrobium, scientific name Dendrobium hancockii Rolfe, is a famous and precious Chinese medicine, which has the functions of nourishing yin and stomach, promoting body fluid and quenching thirst, and has extremely high medicinal value and economic value. [0003] In addition, the ornamental value of Dendrobium officinale is very high, the flowers are elegant, exquisite and lovely, bright in color, and fragrant in smell. [0004] The seeds of Dendrobium candidum are very small, and it is extremely difficult to germinate under natural conditions. However, the traditional method of division and cutting propagation is slow, and it is difficult to meet the market demand. Adopting plant tissue culture technology is an ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 许泉周年英张磊刘欣
Owner NANJING AGRICULTURAL UNIVERSITY
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