Construction method of in-vitro regeneration system of adiantum reniforme var.sinense

A technology of in vitro regeneration and establishment method, which is applied in the field of establishment of an in vitro regeneration system of Pteridopsis lotus leaf to achieve the effect of protecting and alleviating the demand for commercial production

Inactive Publication Date: 2015-06-03
李军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the research on the regeneration system of ferns in China. Therefore, the establishment of an in vit

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) Collection and treatment of explants: Select sporophyll leaves with brown sporangium cover and uncracked sporangia, put them in a dry paper bag, and let them dry naturally. After 5 days, the sporangia cracked naturally, and the spores were scattered in the paper bag. The impurities in the bag were removed, the dried spores were collected and placed in a centrifuge tube, and the centrifuge tube containing the spores was stored in the refrigerator at 2°C for later use.

[0020] (2) Disinfection of spores: Select the spores treated in step (1) and place them in a 1.5mL centrifuge tube, add 1 drop of Tween and appropriate amount of distilled water into the centrifuge tube, cover the centrifuge tube, shake it slightly, and then put it flat Leave it for 4 minutes, then shake the spores in the mixed solution evenly and transfer them to the fast filter paper, catch the filtered waste water with the conical flask through the funnel and quickly place the filter device in the u...

Embodiment 2

[0026] (1) Collection and treatment of explants: Select sporophyll leaves with brown sporangium cover and uncracked sporangia, put them in a dry paper bag, and let them dry naturally. After 6 days, the sporangia cracked naturally, and the spores were scattered in the paper bag. The impurities in the bag were removed, the dried spores were collected and placed in a centrifuge tube, and the centrifuge tube containing the spores was stored in the refrigerator at 4°C for later use.

[0027] (2) Disinfection of spores: Select the spores treated in step (1) and place them in a 1.5mL centrifuge tube, add 2 drops of Tween and an appropriate amount of distilled water into the centrifuge tube, cover the centrifuge tube, shake it slightly, and then put it flat Leave it for 6 minutes, then shake the spores in the mixed solution evenly and transfer them to the fast filter paper, catch the filtered waste water with the conical flask through the funnel and quickly place the filter device in t...

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Abstract

The invention discloses a construction method of an in-vitro regeneration system of adiantum reniforme var.sinense, and relates to a method for regenerating the adiantum reniforme var.sinense through the plant tissue culture technology by the GGB manner. The method is that a mature spore of adiantum reniforme var.sinense is used as an explant, and the steps of disinfecting of the explant, germination cultivating of the spore, propagation and differentiation and the like are performed to build the regeneration system of adiantum reniforme var.sinense. The method is of important significance on the protection of endangered plants and relieving of commercial production demand.

Description

technical field [0001] The invention relates to a plant tissue culture method in plant biotechnology, in particular to a method for establishing an isolated regeneration system of Adiantum lotus leaf. Background technique [0002] Ferns, also known as ferns, originated from the bare ferns of the Silurian and Devonian periods in the Paleozoic Era, and their classification status is between bryophytes and seed plants. Ferns, except for a few with rhizoids, all have differentiation of roots, stems, and leaves, and have vascular tissues inside. They are a group of vascular plants and are considered to be one of the gates of the modern plant kingdom in the current classification system. One, it is an important part of the ancient and modern plant kingdom on earth. In the life history of ferns, there are two independent living plants, the sporophyte and the gametophyte, and there is an obvious alternation of generations. Ferns spread by spores, because the spores are very li...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨惠才
Owner 李军
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