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A method for inducing sporophytes of Adiantum lotus leaf

A technology of fern spores and sporophytes, which is applied in the field of substrate induction and culture of sporophytes of Pteridophyta chinensis, can solve problems such as limited exploration of test materials, and achieve the effects of accelerating the process of breeding experiments, improving the germination rate, and maintaining excellent characters.

Active Publication Date: 2020-06-23
CHINA THREE GORGES CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, both GGB induction and sporophyte germination were carried out in purely inorganic chemical reagent medium, and the exploration of test materials on natural growth substrate conditions was limited.

Method used

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  • A method for inducing sporophytes of Adiantum lotus leaf
  • A method for inducing sporophytes of Adiantum lotus leaf
  • A method for inducing sporophytes of Adiantum lotus leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Explant treatment, prothallus and GGB induction

[0021] Mature spores collected in the current year were sterilized and inoculated on MS + 0.5mg / L 6-BA + 0.1mg / L NAA + 3.0% sucrose + 0.7% agar, with a pH value of 5.5-6.0, and the original spores grew in about 30 days GGB induction was carried out after the leaf body, the induction medium was MS+1.0mg / L 6-BA+0.1mg / L NAA+3.0% sucrose+0.7% agar, the pH value was 5.5-6.0;

[0022] Matrix type selection and ratio

[0023] The type and proportion of the substrate are the key to the successful induction of sporophytes. After the sporophyte induction mixed matrix is ​​prepared according to a certain ratio, add pure water for blending. It is advisable to add water when the matrix is ​​held in the hand and form a group naturally. The thickness of the matrix subpackage is preferably 1-2cm. Bacteria pot sterilization, commonly used pressure 0.15MPa, temperature 121 ℃, sterilization time 20 ~ 40min.

[0024] The mixed matrix mai...

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Abstract

The invention provides a method for inducing sporophores of adiantum reniforme. The method comprises the following steps: inducing prothallium of the adiantum reniforme, culturing the prothallium through an induction culture medium for 58 days after growing the prothallium on mature spores, and then cutting the prothallium into blocks; carrying out induction culturing on the prothallium after cutting on a sporophore induction mixing matrix, thus completing an induction step of the sporophores of the adiantum reniforme. The induction culture medium is a mixture is a MS (Murashige and Skoog) culture medium is prepared from 1.0mg / L of 6-BA (Benzylaminopurine), 0.1mg / L of NAA (Naphthalene Acetic Acid), sugaccharose of which the mass concentration is 3.0 percent and agar of which the mass concentration is 0.7 percent, wherein the pH (Potential of Hydrogen) value of the mixture is 5.5 to 6.0; the sporophore induction mixing matrix is prepared from at least three of river sand, peat, perliteand vermiculite.

Description

technical field [0001] The invention provides a method for inducing culture of Adiantum sporophyte, in particular a matrix induction culture method for sporophyte of Adiantum lotus leaf. Background technique [0002] Adiantum lotus leaf, also known as Adiantum lotus leaf, belongs to the Adiantum unifoliate family. It was collected from Wanxian County, Sichuan Province by Xiong Wanzhong in 1975. It was named a new species of Adiantum lotus leaf by Lin Youxing in 1980. It is the only single-leaved plants. As a relict plant of the Quaternary, he immediately attracted the attention of various botanists, and it is of great significance to the study of the theory of continental drift. In addition, Adiantum lotus leaf also has obvious pharmacological effects on the kidney, liver and urinary system. It has a long history of folk collecting this plant for medicine. At present, folk collection, scientific research use and even commercial operation make this plant face a greater cris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01G24/12A01G24/15A01G24/28A01G31/00A01H4/001A01H4/008
Inventor 张海波黄桂云吴笛张国禹邱利文吴锦华胡梅香望雄英马晓波张俊张定军杨兰芳汪磊李翩翩陈会员
Owner CHINA THREE GORGES CORPORATION
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