Spore propagation method of rare and endangered adiantum reniforme

A technology of Adiantum and spores, which is applied in the field of spore propagation of rare and endangered Adiantum lotus leaf, can solve the problems of low conversion rate, long emergence time, cumbersome procedures, etc., achieves improved emergence time and emergence rate, and simple propagation method easy-to-use effects

Active Publication Date: 2021-07-20
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Xu Huizhu et al. have disclosed a method for sowing and propagating Adiantum spores, using high-quality leaf humus as the sowing substrate, and it takes about 110 days from sowing to the emergence of sporophyte seedlings, but the method is cumbersome and takes a long time to emerge
[0006] Through t

Method used

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  • Spore propagation method of rare and endangered adiantum reniforme
  • Spore propagation method of rare and endangered adiantum reniforme
  • Spore propagation method of rare and endangered adiantum reniforme

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Embodiment 1

[0036] Cultivation substrate, container selection and treatment

[0037] The cultivation substrate is divided into two layers. The upper cultivation substrate is composed of equal volumes of peat and vermiculite. The particle size of peat and vermiculite is less than 2mm, and the water content is 50%. The lower cultivation substrate is three equal volumes of peat, red jade soil and vermiculite. Mixed composition, the particle size is 5mm, and the water content is 50%; the cultivation medium is sterilized at 121°C for 20 minutes; spread 1cm thick water-retaining cotton on the bottom of the breeding box, one of the corners of the water-retaining cotton extends to the mouth of the box, and then the cultivation medium Put them into the breeding boxes one by one and fully infiltrate them with sterile water.

[0038] Spore collection and processing

[0039] Collect mature uncracked Adiantum sporangia leaves from Shizhu County, Chongqing City, put them into a sealed bag made with su...

Embodiment 2

[0044] Cultivation substrate, container selection and treatment

[0045]The cultivation substrate is divided into two layers. The upper cultivation substrate is composed of equal volumes of peat and vermiculite. The particle size of peat and vermiculite is less than 2mm, and the water content is 30%. The lower cultivation substrate is peat, red jade soil and vermiculite. Volume mixed composition, the particle size is 5mm, and the water content is 30%; the cultivation substrate is sterilized at 121°C for 20 minutes; the water-retaining cotton with a thickness of 1.5cm is placed on the bottom of the breeding box, and one of the corners of the water-retaining cotton extends to the mouth of the box, and then put The cultivation substrates were put into the breeding boxes one by one and fully infiltrated with sterile water.

[0046] Spore collection and processing

[0047] Collect mature uncracked Adiantum sporangium leaves from Shizhu County, Chongqing City, put them into a seale...

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Abstract

The invention discloses a spore propagation method for rare and endangered adiantum reniforme. A culture medium adopts double layers, and water retention cotton is added in a breeding box, so that water in the breeding box is balanced during spore propagation; and leaves of the adiantum reniforme which are collected in the field and contain mature spores are dried in a sealed paper bag to enable the spores to naturally fall off, the mature spores are collected, then the spores are disinfected by using a NaClO solution, the disinfected spores are prepared into a spore suspension, and finally the spore suspension is inoculated to the surface of the culture medium in the breeding box is cultured under a certain environmental condition to obtain juvenile sporophytes. According to the method, the steps are simple, the breeding success rate of the rare and endangered adiantum reniforme is effectively increased, the conversion rate of gametophytes of the adiantum reniforme developed into the juvenile sporophytes is increased to 80% or above from original 46.6%, and a technical guarantee is provided for saving, breeding and protecting the adiantum reniforme.

Description

technical field [0001] The invention belongs to the technical field of breeding of medicinal and ornamental plants, and in particular relates to a spore propagation method of rare and endangered Adiantum lotus leaf. Background technique [0002] Adiantum lotus leaf, also known as "lotus leaf money grass", is an evergreen plant of the genus Adiantum in the subfamily Adiantum of Pteridaceae. It is an independent species and the only single plant in the genus Adiantum. Leafy plants. It was first discovered in Wanxian County, Sichuan Province (now Wanzhou District, Chongqing City) in 1977. It is only distributed in local river valleys in Wanzhou District and Shizhu County, Chongqing City. It grows in pieces on rocks covered with thin soil at an altitude of 350 meters. in the seam. It is one of the endemic plants in the Three Gorges area. It is a rare and endangered fern unique to my country. It is listed as a national first-class protected wild plant and a national second-clas...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G24/28A01G24/10A01G24/15A01G24/22A01G24/42A01G9/029A01G13/02A01G27/06
CPCA01G22/00A01G24/28A01G24/10A01G24/15A01G24/22A01G24/42A01G9/0293A01G13/0243A01G27/06Y02P60/21
Inventor 杨庆华李莉孔羽王琦秦俊
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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