Spore propagation method of rare and endangered adiantum reniforme

Abstract

The invention discloses a spore propagation method for rare and endangered adiantum reniforme. A culture medium adopts double layers, and water retention cotton is added in a breeding box, so that water in the breeding box is balanced during spore propagation; and leaves of the adiantum reniforme which are collected in the field and contain mature spores are dried in a sealed paper bag to enable the spores to naturally fall off, the mature spores are collected, then the spores are disinfected by using a NaClO solution, the disinfected spores are prepared into a spore suspension, and finally the spore suspension is inoculated to the surface of the culture medium in the breeding box is cultured under a certain environmental condition to obtain juvenile sporophytes. According to the method, the steps are simple, the breeding success rate of the rare and endangered adiantum reniforme is effectively increased, the conversion rate of gametophytes of the adiantum reniforme developed into the juvenile sporophytes is increased to 80% or above from original 46.6%, and a technical guarantee is provided for saving, breeding and protecting the adiantum reniforme.

Description

technical field [0001] The invention belongs to the technical field of breeding of medicinal and ornamental plants, and in particular relates to a spore propagation method of rare and endangered Adiantum lotus leaf. Background technique [0002] Adiantum lotus leaf, also known as "lotus leaf money grass", is an evergreen plant of the genus Adiantum in the subfamily Adiantum of Pteridaceae. It is an independent species and the only single plant in the genus Adiantum. Leafy plants. It was first discovered in Wanxian County, Sichuan Province (now Wanzhou District, Chongqing City) in 1977. It is only distributed in local river valleys in Wanzhou District and Shizhu County, Chongqing City. It grows in pieces on rocks covered with thin soil at an altitude of 350 meters. in the seam. It is one of the endemic plants in the Three Gorges area. It is a rare and endangered fern unique to my country. It is listed as a national first-class protected wild plant and a national second-clas...

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Problems solved by technology

[0005] Xu Huizhu et al. have disclosed a method for sowing and propagating Adiantum spores, using high-quality leaf humus as the sowing substrate, and it takes about 110 days from sowing to the emergence of sporophyte seedlings, but the method is cumbersome and takes a long time to emerge

[0006] Through the research on tissue culture and rapid propagation of Adiantum lotus leaf, Huang Fang et al. adopted aseptic sowing and tissue culture rapid propagation technology, using prothallus for proliferation, and the conversion rate of gametophyte to young sporophyte was about 20% to 30%. , low conversion rate

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