Preparation method of bacillus subtilis wettable powder

Abstract

The invention relates to the fields of biological pesticide and microbial fertilizer, and specifically discloses a bacillus subtilis wettable powder containing spore germinator and a preparation method thereof. The wettable powder is composed of the following raw materials in parts by weight: 40 to 70 parts of bacillus subtilis fermentation broth, 20 to 30 parts of filling material (diatomite), 1 to 4 parts of wetting agent (Morwet EFW), 0.2 to 1 part of dispersant (CMC-Na), spore germinator: 0.1 to 0.5 part of L-alanine, 0.1 to 0.5 part of inosine, and 0.5 to 1.5 parts of fructose; UV protector: 0.5 to 2 parts of ascorbic acid, and 0.2 to 1 part of lecithin; dryness protector: 1 to 4 parts of maltose and 0.2 to 1 part of methylcellulose, and 1 to 2 parts of synergist (amino oligosaccharin). After the prepared bacillus subtilis wettable powder is applied to the fields, the spore germination speed is accelerated, the germination rate is high, the fertilizing effect and the plant disease control effect are both prominently improved, the using amount of spores is reduced, and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological pesticides and microbial fertilizers, in particular to a Bacillus subtilis wettable powder containing a spore germination agent and a preparation method thereof. Background technique [0002] my country uses a large amount of chemical pesticide preparations and fertilizers every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticide has few side effects and is harmless to humans. It is a kind of green pesticide with broad prospects and has increasingly become a trend in the development of global pesticides. [0003] Bacillus is currently the most studied microorganism in biological pesticides and microbial fertilizers. It has broad-spectrum antibacterial activity and strong resistance to stress. Its vegetative cells can reproduce and colonize on the surface of fungal p...

AI Technical Summary

Problems solved by technology

For example, a mixture (AGFK) made of glucose, fructose, aspartic acid and potassium chloride can be used as a germination agent for the germination of Bacillus subtilis spores, but there is no relevant application of the spore germination agent of Bacillus subtilis to wettability The technical scheme in the powder has been disclosed

[0015] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used The wettable powder of Bacillus subtilis containing spore germination agent was prepared, the adsorption amount of effective bacteria in the wettable powder, the survival rate, the control effect on harmful bacteria and the stability of the wettable powder dose were quite different. The preparation method must fully consider the adsorption capacity of each carrier, the protective effect on the drying process of Bacillus subtilis, the influence of wetting agents and dispersants on the suspension rate of wettable powders, and the wetting time, and a comprehensive screening of spore germination agents. Get a wettable powder for Bacillus subtilis with short wetting time, high suspension rate, long storage time of spores, rapid germination of spores after use, and good control effect