Beauveria bassiana microcapsule formulation

A technology of microcapsules and Beauveria bassiana, which is applied in the directions of insecticides, biocides, animal repellents, etc., can solve the problems such as the decline of the viability of the conidia of Beauveria bassiana, and achieve the improvement of effective utilization rate and resistance to ultraviolet radiation. The effect of high ability and good control effect

Inactive Publication Date: 2012-07-18
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

Secondly, ultraviolet rays in the natural environment can also lead to a decrease in the viability of conidia of Beauveria bassiana

Method used

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  • Beauveria bassiana microcapsule formulation
  • Beauveria bassiana microcapsule formulation
  • Beauveria bassiana microcapsule formulation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Take an appropriate amount of conidia of Beauveria bassiana, mix with 1g kaolin, 1g peptone and water to make 99mL of 107 spores / mL bacterial suspension, then add 1g sodium alginate, stir evenly with a stirrer at 750r / min, pour into 2000mL In the dispersant, the volume ratio of n-hexadecane, corn oil, and lecithin in the dispersant is 7:3:0.05. After stirring for 30 seconds, the emulsified mixed solution is poured into 4000 mL of 0.5M calcium chloride solution. , stirring at 300r / min for 1min, standing for 5min, the cross-linked microcapsules were precipitated in calcium chloride, and then filtered. The above preparation processes are all carried out under normal temperature and pressure.

Embodiment 2

[0028] Take an appropriate amount of Beauveria bassiana conidia, mix 10 g of kaolin, 1 g peptone and water 8 99mL of spore / mL bacterial suspension, then add 1g of sodium alginate, stir evenly with a stirrer at 750r / min, pour into 2000mL of dispersant, the volume ratio of n-hexadecane, corn oil and lecithin in the dispersant 5:5:0.017, continue to stir for 30s, pour the emulsified mixed solution into 4000mL of 1M calcium chloride solution, stir at 300r / min for 1min, let stand for 5min, the crosslinked microcapsules precipitate in the chloride Calcium, can be filtered. The above preparation processes are all carried out under normal temperature and pressure.

[0029] The composition of the dispersant of the present invention is not limited to the above examples, wherein the content ratio of n-hexadecane is 100% v / v, 70% v / v, 50% v / v and 30% v / v, and the corn oil content is 0% v / v, 30% v / v, 50% v / v and 70% v / v, the content of lecithin is 0%, 0.17%, 0.33% and 0.5% of the dispers...

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Abstract

The invention discloses a beauveria bassiana microcapsule formulation which is prepared by taking suspension prepared from beauveria bassiana conidia, kaolin and water as a capsule core, taking sodium alginate as a capsule wall, dispersing in a dispersing agent of n-hexadecane, corn oil and lecithin with different proportions, crosslinking through calcium ions, and solidifying microcapsules to enveloped fine particles with partical diameter being smaller than 100 Mu m. In the germination process of the beauveria bassiana conidia, a germ tube can perforate through the envelop of the capsule wall. The microcapsule formulation is also added with a conidium germination nutrient solution which increases the germination percentage and the germination speed of the conidia. The beauveria bassiana microcapsule formulation can effectively prevent the influences bad environments such as external ultraviolet rays and drying from the conidia, achieves high germination percentage under the influences of the same temperature, humidity and ultraviolet rays, and has good control effect to agriculture and forestry pests; and meanwhile the beauveria bassiana microcapsule formulation has simple production process and is easy for mass production.

Description

technical field [0001] The invention relates to a biological pesticide, in particular to a Beauveria bassiana microcapsule. Background technique [0002] Beauveria bassiana is currently the most widely studied and applied broad-spectrum entomogenic fungus in the world, accounting for 21% of the total number of fungal diseases. Because of its strong pathogenicity, wide insecticidal range, easy cultivation and harmlessness to warm-blooded animals and the environment, it is widely used in agricultural and forestry pest control. [0003] In the early days, there were powders for Beauveria bassiana insecticides in my country, and dusters must be used for powders. The agents are easy to float, have poor adhesion to insects and plants, and the storage period of powders is short. my country has successively invented the following other dosage forms, such as wettable powder, granule, oil, suspoemulsion, etc. Chinese invention patent (publication number CN1539291A) discloses a kind ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/28A01P7/04
Inventor 段立清杨帆段耀特木钦宋钢赵胜国
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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