Beauveria bassiana microcapsule formulation
A technology of microcapsules and Beauveria bassiana, which is applied in the directions of insecticides, biocides, animal repellents, etc., can solve the problems such as the decline of the viability of the conidia of Beauveria bassiana, and achieve the improvement of effective utilization rate and resistance to ultraviolet radiation. The effect of high ability and good control effect
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Embodiment 1
[0026] Take an appropriate amount of conidia of Beauveria bassiana, mix with 1g kaolin, 1g peptone and water to make 99mL of 107 spores / mL bacterial suspension, then add 1g sodium alginate, stir evenly with a stirrer at 750r / min, pour into 2000mL In the dispersant, the volume ratio of n-hexadecane, corn oil, and lecithin in the dispersant is 7:3:0.05. After stirring for 30 seconds, the emulsified mixed solution is poured into 4000 mL of 0.5M calcium chloride solution. , stirring at 300r / min for 1min, standing for 5min, the cross-linked microcapsules were precipitated in calcium chloride, and then filtered. The above preparation processes are all carried out under normal temperature and pressure.
Embodiment 2
[0028] Take an appropriate amount of Beauveria bassiana conidia, mix 10 g of kaolin, 1 g peptone and water 8 99mL of spore / mL bacterial suspension, then add 1g of sodium alginate, stir evenly with a stirrer at 750r / min, pour into 2000mL of dispersant, the volume ratio of n-hexadecane, corn oil and lecithin in the dispersant 5:5:0.017, continue to stir for 30s, pour the emulsified mixed solution into 4000mL of 1M calcium chloride solution, stir at 300r / min for 1min, let stand for 5min, the crosslinked microcapsules precipitate in the chloride Calcium, can be filtered. The above preparation processes are all carried out under normal temperature and pressure.
[0029] The composition of the dispersant of the present invention is not limited to the above examples, wherein the content ratio of n-hexadecane is 100% v / v, 70% v / v, 50% v / v and 30% v / v, and the corn oil content is 0% v / v, 30% v / v, 50% v / v and 70% v / v, the content of lecithin is 0%, 0.17%, 0.33% and 0.5% of the dispers...
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