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Sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds

A hybrid seed and aseptic sowing technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems affecting the breeding efficiency of distant hybridization between orchid species, poor technical repeatability, and large germination differences. , to achieve the effect of high germination rate, high seedling quality and fast germination

Active Publication Date: 2016-06-29
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, the germination of different hybrid embryos is quite different, which directly affects the efficiency of distant hybridization between Orchid species.
In recent years, there have been reports on interspecific hybridization of some orchids in China, but the established techniques have poor repeatability.

Method used

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Embodiment Construction

[0022] The present invention adopts the hybrid orchid 'Miss Taipei' as the female parent, and the wild domesticated Tibetan tiger head orchid as the male parent to carry out aseptic sowing and seedling propagation of interspecific hybrid seeds. The specific steps are as follows:

[0023] (1) Artificial pollination: The hybrid orchid 'Miss Taipei' is used as the female parent and the Tibetan tiger head orchid is used as the male parent. When the two parents bloom at the same time in early December, the flowers that are 2 to 4 days after the female parent blooms are taken and sterilized. The tweezers removed the pollen blocks on the lip and stigma, took the pollen blocks of the fully opened flowers of the male parent and placed them in the cavity of the female stigma; after pollination, hang a label on the female plant, indicating the date of pollination, Hybrid male parent and number of pollinated flowers;

[0024] (2) Fruit pod pretreatment and seed sowing: cut uncracked matur...

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Abstract

The invention provides a sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds. The sterile sowing and seedling raising method comprises the following steps: taking hybrid orchid 'Miss Taipei' as a female parent and Cymbidium tracyanum as a male parent; carrying out artificial pollination to obtain hybrid capsular fruits; and carrying out reproduction steps such as capsule pretreatment, seed sowing, seed germination, synchronous rhizome proliferation and differentiation, rooting and transplantation and acclimatization and transplant to obtain hybrid offspring population. The sterile sowing and seedling raising method for hybrid orchid and Cymbidium tracyanum hybrid seeds has the advantages of high seed germination percentage, large numbers of survived seedlings, short seedling development time, good seedling quality and the like; and the rate of survival reaches 97.8% after the seedlings are transplanted for 60 days, a large number of obtained progeny plants lay a material base for breeding of excellent new species (series) of the hybrid orchid, and meanwhile, an effective new path is provided for production of seedlings of the hybrid orchid.

Description

technical field [0001] The invention relates to a method for distant hybrid breeding and aseptic sowing and propagation of hybrid seeds, in particular to a method for aseptic sowing and seedling cultivation of hybrid orchid and Tibetan tiger head orchid hybrid seeds. Background technique [0002] Hybrid Cymbidium (Hybrid Cymbidium) is a new species of orchids bred by hybridization between Cymbidium and Cymbidium. It has a high ornamental and economic value, and has become a new favorite in the Lunar New Year Festival and daily consumption market in recent years. The consumption share has increased year by year, and the market potential is huge. [0003] Tibetan tiger head orchid (Cymbidium tracyanum L.) is an epiphytic perennial herb of the genus Orchidaceae. It is native to the southwest of Guizhou, Sichuan, southwest to southeast of Yunnan and southeast of Tibet. It is also distributed in Myanmar and Thailand and is now widely cultivated. The leaves are long and green, t...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 钟淮钦陈南川黄敏玲陈少云叶秀仙林榕燕
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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