Preparation method and application of rhamnolipid

A technology for rhamnolipid and seeds is applied in the field of preparation of rhamnolipid, which can solve the problems of large processing workload, unfavorable human health and environmental protection, difficulty in sedimentation, etc., so as to reduce the harm of human body and the environment and inhibit plant growth. Fungal diseases, the effect of reducing sludge disposal

Active Publication Date: 2010-09-29
浙江圣达紫金生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most conventional insecticides are highly toxic, and frequent use is not conducive to human health and environmental protection. As a biological insecticide, rhamnolipid has a significant insecticidal effect. Reports about pesticides
[0008] In addition, the organic matter in the oily sludge of petrochemical enterprises is complex and has different components, which ha

Method used

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  • Preparation method and application of rhamnolipid
  • Preparation method and application of rhamnolipid
  • Preparation method and application of rhamnolipid

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preparation example Construction

[0037] The preparation method of this rhamnolipid, concrete steps are as follows:

[0038] 1. Strain cultivation: Inoculate the Pseudomonas aeruginosa ZJU-211 strain into the shake flask seed medium for cultivation, the temperature is 32-38°C, the shaker speed is 120-200rpm, and cultivate for 24-48 hours to obtain the shake flask strain.

[0039] The composition of the shake flask seed medium (g / L): waste oil, 30-40: NaNO 3 , 2~4; NaCl, 0.5~1.5; KCl, 0.5~1.5; CaCl 2 2H 2 O, 0.05~0.2; KH 2 PO 4 , 2~4; Na 2 HPO 4 12H 2 O, 2~4; MgSO 4 , 0.1~0.3; trace elements, 1.5~2.5mL / L, the balance is water.

[0040] Trace element composition (g / L): FeCl 3 ·6H 2 O 0.01~0.08, ZnSO 4 ·7H 2 O 0.1~0.75, CuSO 4 ·5H 2 O 0.01~0.075, MnSO 4 ·H 2 O 0.1~0.75, H 3 BO 3 , 0.01 ~ 0.15, the balance is water.

[0041] 2. Fermentation: Inoculate the shake flask strains into a fermenter of 10L or an integer multiple thereof, and cultivate with a fermentation medium, the inoculum amount is 3...

Embodiment 1

[0055] Embodiment 1: the preparation of debacterial rhamnolipid

[0056] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosaZJU-211)

[0057] 2. The strain was inoculated in the seed medium at a temperature of 35° C., the rotation speed of the shaker was 180 rpm, and cultured for 48 hours to obtain the shake flask strain.

[0058] The composition of the seed medium is as follows (g / L):

[0059] McDonald's Waste Grease, 30: NaNO 3 , 2; NaCl, 0.5; KCl, 0.5; CaCl 2 2H 2 O, 0.05; KH 2 PO 4 , 2; Na 2 HPO 4 12H 2 O,2; MgSO 4 , 0.1; trace elements, 1.5mL / L, the balance is water. The trace element composition is as follows: FeCl 3 ·6H 2 O 0.01, ZnSO 4 ·7H 2 O 0.1, CuSO 4 ·5H 2 O 0.01, MnSO 4 ·H 2 O 0.1g / L, H 3 BO 3 , 0.01, and the balance is water.

[0060] 3. Production of rhamnolipid by fermentation in 10L fermenter

[0061] The strains in the shake flask were inoculated into a 10L fermenter with an inoculum size of 5% (...

Embodiment 2

[0066] Embodiment 2: the preparation of rhamnolipid concentrate

[0067] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosaZJU-211)

[0068] 2. The strain was inoculated in the seed medium at a temperature of 38° C., the rotation speed of the shaker was 200 rpm, and cultured for 48 hours to obtain the shake flask strain.

[0069] The composition of the seed medium is as follows:

[0070] Rapeseed oil processing leftovers, 40g / L: NaNO 3 , 4g / L; NaCl, 1.5g / L; KCl, 1.5g / L; CaCl 2 2H 2 O, 0.2g / L; KH 2 PO 4 , 4g / L; Na 2 HPO 4 12H 2 O, 4g / L; MgSO 4 , 0.3g / L; trace elements, 2.5mL / L, the balance is water. The trace element composition is as follows: FeCl 3 ·6H 2 O 0.08g / L, ZnSO 4 ·7H 2 O 0.75g / L, CuSO 4 ·5H 2 O 0.075g / L, MnSO 4 ·H 2 O 0.75g / L, H 3 BO 3 , 0.15g / L, and the balance is water.

[0071] 3. Production of rhamnolipid by fermentation in 50L fermenter

[0072] Inoculate the shake flask strain into a 50L fermenter,...

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Abstract

The invention discloses a preparation method and application of rhamnolipid. In the method, the rhamnolipid is produced by using waste grease as carbon source, and utilizing the fermentation of pseudomonas aeruginosa; and with the combination of the defoaming effect of ethanol, the yield of the rhamnolipid can reach 35 to 50 g/L and the cost is about 25 to 40 percent lower than that of the conventional method. When the solution containing 20 to 500 mg/L of rhamnolipid is used for processing plant disease fungus, the ratio of inhibiting disease fungal mycelium growth and spore germination can reach 40 to 90 percent and the plant fungal diseases can be effectively inhibited; the solution containing 20 to 500 mg/L of rhamnolipid has an obvious effect of killing cockroaches and aphids; and in addition, when the rhamnolipid is used as an aid of pesticide, fertilizer and feed and a dehydrating agent of oil-contained sludge by replacing a chemical surface active agent, the effect is obvious.

Description

technical field [0001] The invention relates to the technical field of biosurfactants, in particular to a preparation method of rhamnolipid and its application as a biopesticide, auxiliary agent, insecticide and oily sludge dewatering agent. Background technique [0002] Biosurfactants are a class of surfactants produced by microorganisms, animals or plants, which are very similar in performance to chemically synthesized surfactants, that is, the molecules are composed of polar hydrophilic groups and non-polar lipophilic groups. A molecular layer is formed on the phase interface to reduce the energy of the interface, and it has good surface activity, such as emulsification, dispersion, wetting and penetration. Compared with chemically synthesized surfactants, biosurfactants have stronger surface activity and stronger emulsifying ability due to their complex and large number of chemical groups; they are non-toxic, use less, and are easily degraded by microorganisms. It has v...

Claims

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Application Information

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IPC IPC(8): C12P19/02A01N63/02A01N43/16A01N25/30A01P3/00A01P7/04C05G3/00C02F11/14A23K1/16C12R1/385
Inventor 孟琴张国亮
Owner 浙江圣达紫金生物科技有限公司
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