3 results about "Basophilia" patented technology

This invention discloses a single-cell microarray-based kit and method for in situ quantitative detection of receptor-binding allergen-specific IgE. The kit includes: a single-cell microcavity array chip, an antigen barcode chip, a plastic clamp, IgE standards, fluorescently modified IgE detection antibodies, and auxiliary reagents. During detection, basophil single cells are loaded into the microcavity array chip, lysis buffer is added, and the cells are clamped together with the antigen barcode chip. Low-temperature incubation allows the allergen-specific IgE released from cell lysis to be captured in situ by the antigen on the chip. A sandwich immunoassay is then performed using a fluorescently labeled antibody, and absolute quantification is achieved using a standard curve. This invention is the first to achieve high-throughput, high-sensitivity, multi-component parallel in situ quantitative detection of basophil membrane receptor-binding sIgE at the single-cell level. It can directly reflect the functional sensitization state of effector cells and has advantages such as simple operation, low sample requirement, and no need for complex valve devices.

Provided herein are anti-CD203c antibodies and antigen binding portions linked to a c-Kit inhibitor. Such antibody-drug conjugates can be used to specifically target mast cells and / or basophils in proliferative and inflammatory disorders.

Methods, compositions, and systems for preparing and using pooled biological samples for clinical testing are disclosed. In certain embodiments, the methods and systems may be used to pool donors that provide basophils for clinical testing of chronic spontaneous urticaria.