9 results about "Basophilia" patented technology

Devices, methods, and kits are provided for allergy testing based on basophil activation in a blood sample. Susceptibility of an individual to an allergic reaction to an allergen is detected by collecting a blood sample from the individual and assaying for activation of basophils in response to stimulation with an allergen. In particular, a microfluidic device is provided for automating the assay for detecting basophil activation as an indication of the susceptibility of an individual to an allergic reaction as well as kits containing such a device and diagnostic methods for using such a device for allergy testing. Additionally, such a microfluidic device can be adapted for multiplexed detection of allergic responses to multiple allergens by performing assays in parallel to detect the basophil responses to each allergen.

An in vitro method for determining basophil activation in a sample is provided. Other aspects relate to determining an efficacy of an allergen-specific desensitization treatment of a subject. The method includes providing a sample from the subject, treating the sample with an allergen and determining a level of at least one basophil inhibitory marker and at least one basophil activation marker in the sample, the basophil inhibitory marker includes CD32. Corresponding systems and machine learning methods are also described. The use of an affinity reagent that specifically binds CD32 in an in vitro method for determining basophil activation is also provided, for example in the context of determining an efficacy of an allergen-specific desensitization treatment of a subject. A kit and corresponding uses of affinity reagents is also detailed.

Disclosed are methods, compositions and systems for making and using pooled biological samples for laboratory' testing. In certain embodiments, the methods and systems may be used for pooling basophil donors for laboratory testing for chronic spontaneous urticaria.

This invention discloses a single-cell microarray-based kit and method for in situ quantitative detection of receptor-binding allergen-specific IgE. The kit includes: a single-cell microcavity array chip, an antigen barcode chip, a plastic clamp, IgE standards, fluorescently modified IgE detection antibodies, and auxiliary reagents. During detection, basophil single cells are loaded into the microcavity array chip, lysis buffer is added, and the cells are clamped together with the antigen barcode chip. Low-temperature incubation allows the allergen-specific IgE released from cell lysis to be captured in situ by the antigen on the chip. A sandwich immunoassay is then performed using a fluorescently labeled antibody, and absolute quantification is achieved using a standard curve. This invention is the first to achieve high-throughput, high-sensitivity, multi-component parallel in situ quantitative detection of basophil membrane receptor-binding sIgE at the single-cell level. It can directly reflect the functional sensitization state of effector cells and has advantages such as simple operation, low sample requirement, and no need for complex valve devices.

The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and / or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.

Disclosed are methods, compositions and systems for making and using pooled biological samples for laboratory testing. In certain embodiments, the methods and systems may be used for pooling basophil donors for laboratory testing for chronic spontaneous urticaria.

To provide methods of reducing type 2 cytokine-mediated inflammation using neuromedin peptides.SOLUTION: Methods are provided for reducing type 2 cytokine-mediated inflammation, for example by reducing IL-5 and Il-13 activity, using native and variant neuromedin B (Nmb) peptides or coding sequences. Such methods can be used to treat an inflammatory disorder, such as asthma, COPD or an allergic reaction. Also provided are modified Nmb peptides. It is shown herein that Nb-induced ILC2 responses are exaggerated in the absence of basophils resulting in increased inflammation and reduced lung function.SELECTED DRAWING: None

Provided herein are anti-CD203c antibodies and antigen binding portions linked to a c-Kit inhibitor. Such antibody-drug conjugates can be used to specifically target mast cells and / or basophils in proliferative and inflammatory disorders.

Methods, compositions, and systems for preparing and using pooled biological samples for clinical testing are disclosed. In certain embodiments, the methods and systems may be used to pool donors that provide basophils for clinical testing of chronic spontaneous urticaria.